AB Ref
Archaea
phylum
class
order
family
genus
Bacteria
phylum
class
order
family
genus
Eukarya
phylum
class
order
family
genus
Viruses
family
454 RAST-a 454 RAST-b 454 RAST-c 454 IMG-M
Ilmn RAST
3
9
10
12
12
5
12
20
30
72
5
12
20
29
71
3
9
10
11
17
3
9
x
18
x
3
7
8
8
9
17
22
29
34
36
28
43
91
215
648
28
43
91
206
635
20
26
48
65
115
23
33
x
182
x
19
26
51
72
102
0
0
0
0
0
18
52
89
119
170
13
35
59
76
103
2
2
2
2
2
5
x
x
10
x
1
2
3
3
3
0
5
4
2
3
2
Table S2. Taxonomic distribution of Archaea-Bacteria community sequences
based on MG-RAST and IMG-M analysis. The AB Ref column indicates the number of
actual taxonomic levels (phyla to genus) represented by the organisms included in the
synthetic community. The 454 sequence data was analyzed using both MG-RAST
(RAST) and IMG-M. For MG-RAST, three cutoff parameters were used: (a, default) max
e-value: 1e-5, min% identity: 60%, min alignment length: 15; (b) max e-value: 1e-10,
min% identity: 60%, min alignment length: 15; (c) max e-value: 1e-20, min% identity:
80%, min alignment length: 50; For IMG-M analysis there are parameter options and the
taxonomic richness was calculated based on the database output (x indicates taxonomic
levels not available in the output). The Illumina reads were analyzed using the default
parameters in MG-RAST (max e-value: 1e-5, min% identity: 60%, min alignment length:
15;).