Fig. 1 Linkage maps of chromosome 5H in the NB3437f/OR71 and NB713/OR71 doubled haploid
(DH) mapping populations and the 5H quantitative trait locus (QTL) scans for low temperature
tolerance (LTT) as measured in a controlled freeze test at the Martonvasar Research Institute
aligned with the 5H consensus map. Single nucleotide polymorphism (SNP) marker names are
indicated on the left of each linkage group. Distances are given in Haldane cM on the right. The
distances on the two DH maps are continuous with distances between unlinked groups (gaps)
based on the consensus map
Consensus Map
Close et al. (2009)
% Survival
0
1
2
LOD
NB713XOR71
3
4
5
3_0543
2_0226
3_0714
3_0167
0
20
0.0
2.5
10.3
16.3
40
60
80
100
160
180
1_0094
2_0653
233
FR-H1
223
194.1
199.2
1_1490
1_1497
1_0820
223.4
224.2
229.3
2_0536
1_0736
3_0577
1_1364
1_0401
260.5
264.8
268.2
273.3
275.8
243
253
263
273
% Survival
NB3437fXOR71
0
2_1221
2_0206
2_0010
3_0167
3_0531
3_0654
1_1260
2_1536
3_0538
2_1275
3_0080
2_1133
2_0096
2_0645
0.0
0.9
12.0
19.7
24.5
43.0
45.8
49.5
51.4
54.1
59.9
65.6
68.4
74.1
1
2
LOD
3
4
5
0
10
20
43
53
63
73
83
93
3_0850
2_0795
111.1
113.8
3_1278
129.4
2_0653
143.1
1_1490
167.3
103
113
129
167
177
187
197
3_0162
214.7
2_0686
3_0577
1_1364
1_0401
224.7
235.2
236.1
238.0
207
217
235
Fig. 2 Phenotypic frequency distributions for low temperature tolerance (LTT) evaluated in the
NB3437f/OR71 and NB713/OR71 doubled haploid populations in a controlled freeze test at the
Martonvasar Research Institute (MRI) and under field conditions in St. Paul, Minnesota (SPMN).
Standard deviations are shown for the parents. Broad-sense heritability (H2) is also noted
FR-H1
FR-H2
140
63.8
68.9
73.9
77.3
80.6
86.6
87.4
94.3
99.4
102.8
116.6
128.5
132.7
139.6
142.9
145.5
153.3
157.6
0.0
2.1
2.8
6.4
9.3
18.7
19.4
26.3
27.7
46.2
52.0
56.8
59.4
63.3
67.5
69.9
72.3
75.4
80.6
84.5
87.4
91.6
100.3
102.1
103.9
105.2
108.2
108.6
113.1
113.8
122.4
124.2
129.4
153.6
155.1
159.1
161.6
172.4
177.7
180.7
182.9
189.6
192.0
196.9
FR-H2
120
3_0654 1_1260
2_1536
3_0538
1_1281
2_1275
2_0350
1_0953
2_1133
2_0096
3_0314
3_1361
3_0834
2_0850
1_0414
2_0403
2_1314
3_0456
3_0619
3_0163
2_0226
3_0543
2_0206
2_1221
2_0010
3_0714
3_0167
3_0531
3_0654
1_1260
2_1536
3_0538
1_1281
2_1275
3_0080
2_0350
1_0953
2_1133
2_0096
3_0314
3_1361
3_0834
2_0850
1_0414
2_0403
3_0850
2_0795
3_0456
3_0619
1_0094
3_1278
2_0653
1_1490
1_1497
1_0820
3_0162
2_0686
2_0536
1_0736
3_0577
1_1364
1_0401
3_1123
NB713XOR71 GH LTT MRI
H2 = 0.92
40
OR71
(57.8 ± 5.4)
Number of Lines
Number of Lines
NB3437fXOR71 LTT MRI
35
30
25
20
15
10
5
0
NB3437f
(86.9 ± 4.1)
NB713
(73.3 ± 3.5)
30
OR71
(41.2 ± 3.1)
20
10
0
20
30
40
50
60
70
80
90
100
10
20
30
40
Survival (%)
OR71
(16 ± 7.8)
60
70
80
NB713XOR71 LTT SPMN
H2 = 0.87
50
NB3437f
(100 ± 0)
20
Number of Lines
25
50
90
100
Survival (%)
NB3437fXOR71 LTT SPMN
Number of Lines
H2 = 0.92
15
10
5
0
H2 = 0.64
NB713
(100 ± 0)
40
30
OR71
(6 ± 1.1)
20
10
0
0
10
20
30
40
50
60
Survival (%)
70
80
90
100
0
10
20
30
40
50
60
70
80
90
100
Survival (%)
Fig. 3 Quantitative trait locus (QTL) scans on chromosome 1H for low temperature tolerance (LTT) at St.
Paul, Minnesota (SPMN) and LTT from the Martonvasar Research Institute (MRI) freeze test. A 20 cM
scan window was used for this analysis, which is based on the combined map of two doubled haploid
mapping populations: NB3437f/OR71 and NB713/OR71
25
20
LOD
15
SPMN Surival
10
MRI Survival
5
0
0
19
39
59
77
96
116
135
155
173
1H (cM)
Fig. 4 Phenotypic frequency distributions for final leaf number (FLN) evaluated for NB3437f/OR71 and
NB713/OR71 doubled haploid populations. FLN were measured with and without vernalization (V+, V-)
under greenhouse conditions. Standard deviations are shown for the parents when replicated. Broadsense heritability (H2) is also noted
NB3437fXOR71 FLN GH (V+)
25
Number of Lines
Number of Lines
NB713XOR71 FLN GH (V+)
H2 = 0.93
NB3437f
OR71
(10.5 ± 0.7) (11 ± 0)
30
20
15
10
5
0
8
9
10
11
12
13
14
NB713
(11.5 ± 0.7)
35
30
25
20
15
10
5
0
15
8
9
10
Final Leaf Number
25
Number of Lines
Number of Lines
13
14
NB713XOR71 FLN GH (V-)
H2 = 0.67
NB3437f
(12 ± 0)
40
12
15
Final Leaf Number
NB3437fXOR71 FLN GH (V-)
50
11
H2 = 0.92
OR71
(11.5 ± 0.7)
OR71
30
20
10
0
OR71
(13.5 ± 0.7)
20
H2 = 0.91
NB713
(15 ± 0)
15
10
5
0
10
11
12
13
14
Final Leaf Number
15
16
17
9
10
11
12
13
14
15
Final Leaf Number
16
17
18