Data S1: Limits of detection (LOD) and quantification (LOQ) of ME-glucosides and ion
suppression of MEP pathway intermediates
ME-glucosides
Retention time, extraction efficiency, limit of detection (LOD), limit of quantification (LOQ)
and the absolute amounts of MEGlu in wild type Arabidopsis thaliana leaf extracts. The
extraction efficiency values represents the average ± SE (n = 3). Due to the unavailability of
isotopically labelled standards for ME-glu and the absence of 13C label incorporation during
short term 13CO2 labelling, the extraction efficiency and absolute amounts were determined
using a standard addition curve. For the standard addition curve a similar amount of both
isomers were added in triplicate at three known amounts to approximate 75%, 150% and
200% of the ME-glu amount as calculated using an external standard curve. The LOD was
taken as the amount of metabolite detected that gave a signal to noise (S/N) ratio of 3 and the
LOQ was taken as the amount necessary to give an S/N ratio of 5.
Retention time (min)
Extraction efficiency (%)
LOD (pmol ml-1)
LOQ (pmol ml-1)
Absolute amounts (nmol mg-1 DW)
MEGlu
4.0
14.8 ± 0.75
44.9 ± 1.9
74.8 ± 3.2
2.639 ± 0.09
MEP pathway intermediates
Retention time, extraction efficiency, ion suppression, limit of detection (LOD) and limit of
quantification (LOQ) of DXP, MEcDP and DMADP/IDP in Arabidopsis thaliana leaf
extracts. The extraction efficiency and ion suppression values represents the average ± SE (n
= 10). The LOD was taken as the amount of metabolite detected that gave a signal to noise
(S/N) ratio of 3 and the LOQ was taken as the amount necessary to give an S/N ratio of 5.
Retention time (min)
Extraction efficiency (%)
Ion suppression (%)
LOD (pmol ml-1)
LOQ (pmol ml-1)
DXP
6.9
54.0 ± 2.6
105.5 ± 5.7
7.8 ± 0.2
13.0 ± 0.3
MEcDP
6.0
47.8 ± 2.5
55.5 ± 4.0
7.0 ± 0.03
11.6 ± 0.04
DMADP/IDP
6.6
14.2 ± 1.0
76.5 ± 1.3
1.4 ± 0.01
2.3 ± 0.01