1
2
3
4
Fig. S1 Estimated ratios of plastid DNA (ptDNA) to nuclear DNA (nuDNA) in various tissues of
5
maize seedlings. The estimation was based on the real-time quantitative polymerase chain
6
reaction analysis . Regions C23C24 (amplified by the primer pair C23 and C24) (a) and C27C28
7
(amplified by the primer pair C27 and C28) (b) were two ptDNA regions; each had a single copy
8
on the plastid genome. Ptd, Pt: plastid. Nu: nuclear. B96: maize cultivar 39B96. Z69: maize
1
9
cultivar 39Z69. Lf: mainly the first true leaf of the seedlings, and in some cases the second true
10
leaf if it was also open. Root tip: the primary root tip. Rt e-zone: the primary root elongation
11
zone. Exp1: experiment 1. Exp2: experiment 2. Bars indicate standard error based on the two
12
biological experiments and the three technical repeats per experiment. In the Exp1+Exp2
13
summary area, means with the same letter are not significantly different according to factorial
14
analysis of variance and Duncan’s multiple range test. The double asterisks (**) indicate
15
difference from the root tip at the P < 0.01 level according to Student’s t-test. Note that the
16
moderately mature parts of the leaves and roots showed higher ptDNA/nuDNA ratios than did
17
the less mature parts.
18
19
2
20
21
22
23
Fig. S2 Estimated ratios of mitochondrial DNA (mtDNA) to nuclear DNA (nuDNA) in various
24
tissues of maize seedlings. The estimation was based on the real-time quantitative polymerase
25
chain reaction analysis. Regions C33C34 (amplified by the primer pair C33 and C34) (a) and
26
C35C36 (amplified by the primer pair C35 and C36) (b) were two unique mtDNA regions. Each
27
of these regions likely represented a subgenomic molecule, because their copy numbers per cell
3
28
were very different. Mt: Mitochondrial. Nu: nuclear. B96: maize cultivar 39B96. Z69: maize
29
cultivar 39Z69. Lf: mainly the first true leaf of the seedlings, and in some cases the second true
30
leaf if it was also open. Root tip: the primary root tip. Rt e-zone: the primary root elongation
31
zone. Exp1: experiment 1. Exp2: experiment 2. Bars indicate standard error based on the two
32
biological experiments and the three technical repeats per experiment. In the Exp1+Exp2
33
summary area, means with the same letter are not significantly different according to factorial
34
analysis of variance and Duncan’s multiple range test. Note that in leaves, the mature or
35
moderately mature parts showed dramatically lower mtDNA/nuDNA ratios than did the less
36
mature, light-green parts.
37
38
4