Additional files
Suppl. Figure 1 - Chromosomal localization of COS marker
TC176835 using disomic wheat-rye addition lines.
Lanes: (1) H2O, (2) T. aestivum cv. 'Chinese Spring', (3) S. cereale cv. 'Imperial',
(4) 21"+1R", (5) 21"+1RS", (6) 21"+1RL", (7) 21"+2R´/21"+2R´+2RL´, (8)
21"+2RL", (9) 21"+3R", (10) 21"+3RS", (11) 21"+4R", (12) 21"+4RS", (13)
21"+4RL", (14) 21"+5R", (15) 21"+5RS", (16) 21"+5RL", (17) 21"+6R", (18)
21"+6RS", (19) 21"+6RL", (20) 21"+7R", (21) 21"+7RS", (22) 21"+7RL", (23) S.
cereale line L301-N, (24) S. cereale line L7.
Suppl. Tab. 1 – PCR primers and conditions for the 4RL COS
markers.
a)
Designated according to EST consensus identifiers deposited in the TIGR.
Suppl. Table 2 - Summary of the COS-marker development for the
Rfp1 region.
a
Rye markers with best sequence identity to rice genes were designated according to
EST consensus identifiers deposited in the TIGR.
b
RFLP probe identifier.
c
The TIGR identifier for rice genes.
d
E value of the pairwise BLASTN comparison between wheat or barley EST unigene and
rice genes.
e
The identifier for Brachypodium distachion (Bd) and Sorghum bicolor (Sb) genes
orthologous to the corresponding rice gene.
f
GenBank Acc. N° of the member the EST consensus sequence, which has been
physically mapped in wheat.
g
Wheat bin name of the mapped EST according to the GrainGenes-SQL database.
h
EST marker mapped in barley according to Mayer et al. (2011).
i
RE: restriction enzyme used in case of invariant amplicon.
1
j
observed fragment sizes [bp] of the non-restorer allele (A) and the restorer allele (B)
segregating in the mapping populations.
% : no ortholog identified
Suppl. Tab. 3 – Survey on QTL for yield and yield components
deposited in the Gramene database for the a segment on rice
chromosome 2 and 6 orthologous to rye chromosome 4RL.
2