Supplemental Material 1. Table of Contents
Figure S1. Time-course of optical density (), concentration of dissolved oxygen ()
and molar fraction of CO2 () in glucose-limited chemostat cultures of Salmonella
typhimurium LT2 growing at dilution rate of A) D = 0.1 h- l; B) D = 0.15 h- l; C) D = 0.48
and 0.24 h- l; E) D = 0.67 and 0.58 h- l
Figure S2. Fluxes of DNA () during S. typhimurium glucose-limited aerobic chemostat
cultivations under different dilution rates.
Text S1. Methodology for quantification of DNA concentration
Figure S1. Time-course of optical density (), concentration of dissolved oxygen ()
and molar fraction of CO2 () in glucose-limited chemostat cultures of Salmonella
typhimurium LT2 growing at dilution rate of A) D = 0.1 h- l; B) D = 0.15 h- l; C) D = 0.48
and 0.24 h- l; E) D = 0.67 and 0.58 h- l
A
-1
Dilution rate = 0.1 h and Air flow rate = 0.8 SLPM
8
100
1.2
90
1.0
6
OD600nm
70
5
60
4
50
DOC (%)
80
0.8
0.6
yCO2 (%)
7
40
3
0.4
30
2
20
1
0.2
10
0
0
10
20
30
40
0
60
50
0.0
Time (h)
-1
Dilution rate = 0.15 h and Air flow rate = 1.1 SLPM
8
100
7
90
6
80
60
4
3
1.0
0.8
50
0.6
40
0.4
2
30
yCO2 (%)
70
5
1.4
1.2
DOC (%)
OD600nm
B
0.2
1
20
0
0.0
0
5
10
15
20
25
30
35
Time (h)
-1
100
7
90
6
80
70
5
60
4
3
1.4
1.2
1.0
0.8
50
0.6
40
0.4
2
30
1
0.2
20
0
0.0
0
5
10
15
Time (h)
20
25
30
yCO2 (%)
OD600nm
8
-1
Dilution rate = 0.48 h
Dilution rate = 0.24 h
Air flow rate = 1.3 SLPM
DOC (%)
C
-1
Dilution rate = 0.67 h
Dilution rate = 0.58 h
Air flow rate = 1.5 SLPM
D
7
-1
100
1.2
90
6
1.0
70
4
60
3
50
2
40
0.8
0.6
yCO2 (%)
OD600nm
5
DOC (%)
80
0.4
30
1
0.2
20
0
0.0
0
5
10
15
20
Time (h)
Figure S2. Fluxes of DNA () during S. typhimurium glucose-limited aerobic
30
-1
-1
Fluxes of DNA (mmol DNA g DCW h )
chemostat cultivations under different dilution rates.
25
20
15
10
5
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
-1
D (h )
Text S1. Methodology for quantification of DNA concentration
DNA quantification using UV absorbance spectroscopy at 260 nm
DNA concentration measurements in supernatant samples, obtained in aerobic
chemostat with glucose, were performed as described by Heptinstall and Rapley (2000).
Absorption of DNA is maximal at a wavelength of 260 nm, and DNA concentration may
be calculated from Eq A:
50 * A260nm of sample = concentration of DNA (mg L-1)
(A)
Degree of purity of DNA samples were evaluated by examining the absorption at
a wavelength of 280 nm, in which protein has known absorption maxima. The ratio
between A260nm and A280nm must be greater than 1.8, otherwise, it indicates some
contamination by proteins, impairing the DNA quantification.
Literature Cited
Heptinstall J, Rapley R. Spectrophotometric analysis of nucleic acids. In The
nucleic acid protocols handbook. Humana Press. 2000; 57 - 60.