University of Puerto Rico
Río Piedras Campus
College of Natural Sciences
Nutrition and Dietetics Program
Program: Bachelor of Science in
Nutrition and Dietetics
Human Biochemistry Laboratory, NUTR
4159
Second Semester, 2012-2013 Academic Year
Sec. 0U1, Friday, 1:00 p.m. – 3:50 p.m.
Room NEP 577
Michelle Schelske Santos, Ph.D.; mschelskeuprrp@gmail.com, mschelske@uprrp.edu; ext. 5798, 2792
Date
Assignment
11Jan13
Syllabus, Preparation of LAB Notebooks;
Register for course in Edu 2.0
General Laboratory Safety; Intro to
instrumentation; Equipment assignment
(bring lab coats and lab notebooks)
LAB Excel workshop – Data Tables and Graphs
Micropipette and Pipette Techniques
Quiz in Edu2.0 (Safety & Scientific Instrumentation)
open 24h before lab
LAB Expt: Active Acidity and pH;
Pre-lab Quiz in Edu2.0 open 24h before lab
LAB Safety Seminar (Biohazard)
Post-conference written quiz; practical lab quiz
Micropipette and Pipette Techniques
LAB Expt. – Membrane Structure and Function;
Quiz in Blackboard; open 24h before lab;
LAB Computer Workshop – Info Databases and
your Search Strategy (Biblioteca Ciencias
Naturales; Prof. Pura Centeno) Assign: Search
Strategies, DNA & Protein Methods
LAB – DNA Extraction
LAB – Tissue Homogenation of Protein Samples
Quiz in Edu 2.0; open 24h before lab
Due: Assig Search Strategies DNA/Ptn Method
LAB – Total Protein Determination
Quiz in Edu 2.0; open 24h before lab
LAB - DNA Extraction
(need confirmation of nurse collaboration from
Medical Services)
18Jan13
25Feb13
01Feb13
08Feb13
15Feb13
22Feb13
01Mar13
08Mar13
15Mar13
Topic
Introduction to course
Introduction to Prep of Lab Notebook
Lab Safety
Scientific Instrumentation
Introduction to Excel for Lab Reports--Water
content of foods
Micropipette and Pipetting Techniques
Active Acidity and pH
Blood-borne Pathogens and Biohazard
Disposal (OPASO; Dr. Warner Ithier)
Micropipette and Pipette Techniques
Blood Typing
Digital Microscopy of Blood Cells
Biochemical methods for DNA and protein
analyses in the field of nutrition
DNA Extraction from strawberries
Homogenation of animal & vegetable protein
Protein content of animal versus vegetable
protein on microscale using microplate reader
DNA Extraction from whole human blood
Preparation of blood fractions for protein
analysis via SDS-PAGE
Dra. M. Schelske Santos; NUTR 4159
22Mar13
Academic Recess – Abolición de la Esclavitud
29Mar13
Academic Recess – Good Friday (Viernes Santo)
W, 03Apr13
LAB OP1 – (50 pts.; 1h)
Hora universal (11:30 – 12:30pm) o 1:00 – 2:00pm
LAB: Electrophoresis of DNA
05Abr13
12Apr13
19Apr13
EB
26Apr13
03May13
TBA, May 07
-14, 2013
LAB: Protein Electrophoresis of blood fractions
Quiz in Edu 2.0; open 24h before lab
Measurements of migration distances in gels
Bioinformatics Assignment
LAB –Glycolytic fermentation
Due SDS-PAGE Lab (in notebook)
Due Bioinformatics (Edu 2.0)
LAB – Lipids and Fiber
Quiz in Edu 2.0; open 24h before lab
Lab Safety – Total Protein Determination
(08mar13)
Horizontal agarose gel electrophoresis of
nucleic acids
SDS-PAGE or protein electrophoresis, gel
staining & drying
SDS-PAGE and DNA gel interpretation
3-D structure of proteins, genetic diseases
Anaerobic fermentation of sugars by yeast;
mono- and di-saccharides, aspartame,
sucralose and citric acid inhibition of enzyme
Dietary Lipids, Fiber & Fattaché
Lab OP 2 – (50 pts.; 1h)
Due: Lab Notebooks
XXXXXXX
GMO Investigator
XXXXXXX
Colorimetric Determination of Vitamin C
XXXXXXX
Enzyme-linked ImmunoSorbent Assay (ELISA)
Antigens, Antibodies and Enzymes
Identification of Genetically Modified Foods
using PCR (need collaboration for PCR)
Vitamin C Content in Raw vs. Processed
Foods; Comparisons to Food Labels and
Nutrient Data Base
Colorimetric Determination of Presence of
Specific Antigen using Antibodies & Enzymes
This schedule is subject to change based on unforeseen events, natural disasters, scheduling of student
assemblies, and changes in laboratory equipment and material arrival dates. In the event of any change, a new
schedule will be developed and available in Edu 2.0.
sem. 2, 2012-2013
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