PI Name: ENTER NAME
Laboratory-Specific Biosafety Manual
This lab-specific manual applies to the following BSL-2 agents:
1. Enter agent
2. Enter agent
3. Enter agent
4. Enter agent
5. Enter agent
Tekechia Hester
Research Compliance Services
Horsebarn Hill Road, Unit 4097
STORRS, CT 06269-1246
PHONE 860.486.1838
FAX 860.486.1106
ibc@uconn.edu
INSTRUCTIONS
This template is provided to assist Principal Investigators (PIs) in the development of a laboratory-specific
biosafety manual (LSBM) with instructions to safely handle and manipulate a particular agent(s) or
toxin(s) under Biosafety Level 2 (BSL-2) laboratory conditions. In addition to UConn IBC policies, this
manual is also a requirement of the NIH Guidelines (section G-II-B-2-m) and the CDC’s Biosafety in
Microbiological and Biomedical Laboratories, 5th Edition. This document must also outline general
practices for maintaining good laboratory practices, as well as providing response procedures in the event
of an incident. The PI is responsible for including basic background information for each agent (an
example has been provided on page), writing an exposure risk, detailing surface decontamination, and
writing standard operating procedures for experiments where safety is a concern. If SOPs have been
previously drafted then they should be incorporated in the SOPs section of the binder.
Also, please provide lab-specific information where you see italics. Additions and changes to this
template that will render the final manual more useful for the laboratory’s safety needs are encouraged.
Although this template has been provided at a minimum the manual should include the following:
1. Lab contact information, including after-hours number
2. Relevant sections of the Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5thEdition,
(e.g. Sections I, II, and III pertaining to standard BSL-2 practices, Appendix H, pertaining to work with
Human cells and tissues, if applicable).
http://www.cdc.gov/biosafety/publications/index.htm
3. Agent summaries
4. Project specific SOPs
5. Laboratory specific policies, including:
a. PPE required for working in the lab
b. Infectious Waste Disposal procedures for:
i. Liquids
ii. Sharps
iii. Solids
iv. Research Animals (if applicable)
6. Emergency Evacuation Route
7. Spill Clean-Up Procedures
8. Exposure Response Procedure
9. Copy of current IBC approved registration and approval letter(s)
10. Copies of recent laboratory inspection report(s)
Please modify this document to fit the needs of your laboratory.
A loose-leaf binder that can easily accommodate changes or new materials is the recommended
means for maintaining and organizing this Laboratory-Specific Biosafety Manual.
1
Table of Contents UPDATE AS NEEDED
Lab Contacts and Training ....................................................................................................... 3
Location of Laboratory Emergency Equiptment ........................................................................ 4
Exposure Response Procedures .............................................................................................. 5
Accidental Spill Cleanup Procedures ....................................................................................... 6
Spill Inside the BSC ................................................................................................................. 6
Spill Outside the BSC………………………………………………………………………...6
Spill Kits. .........................................................................................................................7
Medical Treatment Locations…………………………………………………………………....7
Example Agent Writeup: Adenovirus. ..............................................................................8
Agent:
. ...............................................................................................................12
Specific Protocols/SOPs ....................................................................................................... 15
Appendix I: Contact Information…………………………………………………………………….16
Appendix II: Spill Response Cue Cards.................................................................................. 17
Appendix III: Training Certificates.......................................................................................... .19
Appendix IV: IBC Registration(s) & Approval Letters .............................................................. 20
Appendix V: Recent Laboratory Inspection Report(s) ............................................................. 21
Post Approval Monitoring Report ..........................................................................
Biosafety Audit Report ..........................................................................................
Appendix VI: Copy Corrective Action Forms .......................................................................... 22
Appendix VII: Copy Incident Reporting Form………………………………………………………23
Appendix VIII: Lab Door Signage ……..……………………………………………………….……24
2
Lab Contacts and Training
Principal Investigator:
Lab Locations:
Office Phone:
24/7 contact (cell phone):
ENTER HERE
ENTER HERE
ENTER HERE
ENTER HERE
ENTER HERE
ENTER HERE
ENTER HERE
ENTER HERE
ENTER HERE
Copies of completed training certificates or email completion message should be included in
this document as an Appendix III. The table below is used as a tool to help you keep track of
laboratory personnel training requirements.
Laboratory personnel
Name
Training Dates
Bloodborne
Pathogens
Biosafety
General
Chemical
NIH
Safety
Guidelines
Other
Other
Other
3
Location of Laboratory Emergency Equipment
Be sure to provide the locations of equipment for all laboratories research will be
conducted in.
Room #:
ENTER HERE
Nearest Emergency Exit
Room #:
Room #:
Room #:
ENTER HERE
ENTER HERE
ENTER HERE
ENTER HERE
ENTER HERE
Eyewash
Emergency Shower
ENTER HERE
ENTER HERE
Spill Supplies
ENTER HERE
First Aid Kit
Evacuation Rally Point
ENTER HERE
** Personal Protective Equipment (PPE), such as gloves,
lab coat, and safety glasses/goggles are to be worn at ALL
TIMES, except when working at the computer desk**
Wear closed-toe shoes at all times!
Tie up long hair when working with open flames!
** See UConn Biological Safety Manual for BSL-2 requirements
4
EXPOSURE RESPONSE PROCEDURES
Steps to consider with a biohazardous material exposure:
1. Contaminated PPE should be removed
2. Proceed to the closest sink.
3. If the exposure involves broken/compromised skin, (needle sticks and/or sharps) the
area should be washed thoroughly with soap and water for 15 minutes.
4. If applicable cover the wound with a bandage
5. If the exposure involves the contamination of the mucus membrane(s), the area
should be treated by flushing with water for 15 minutes at the nearest emergency
eyewash station
6. Report to Student Health Services (students), UConn Urgent Care, Storrs Center
(faculty and staff), or Windham Hospital (after hours emergencies) for post exposure
follow-up. Provide the medical provider with the following description:

Biohazardous material

The route of exposure (needlestick or splash to eyes)

Time and place of the incident

Prior first aid provided
7. Notify the PI and BSO immediately once you have received medical attention

The PI should fill out an Incident Reporting Form and submit it to the IBC within
5 days of the incident. Forward to ibc@uconn.edu.
ACCIDENTAL SPILL CLEANUP PROCEDURES
Spill inside the BSC:
1. Lower the BSC sash for 5 minutes to allow the blower to move aerosols through the
HEPA filter.

Check to make sure the spill is fully contained in the BSC

If a spill has occurred outside the BSC proceed to, Spills outside the BSC
2. Decontaminate the surface wearing appropriate PPE.
3. Gently cover the spill with absorbent paper towels.
4. Soak with appropriate disinfectant and allow to sit for 20 minutes or the recommended
inactivation time; see agent specific section of this document.
5. Deposit paper towels as biowaste. *
5
6. Residual bleach can be wiped off with 70% EtOH, and disposed as biowaste.
Spills outside the BSC of Biohazardous Material:
1. Notify staff in the laboratory that a spill has occurred and evacuate the room
immediately. All PPE and contaminated clothing should be removed upon exiting.

Be sure to have the last person exiting close the door on the way out. Prohibit
others from entering the room, either by verbal communication or door signage.

Notify the PI and Biosafety Officer

If an overt exposure has occurred follow procedures for Biohazardous Material
Exposure Response
2. Do not re-enter for at least 30 minutes to allow aerosols to settle, reducing the risk of
inhalation exposure.
3. Don clean PPE, cover with absorbent paper towel.
4. Starting from the perimeter and working your way toward the center, soak with agent
specific inactivation disinfectant (i.e. 10% Sodium Hypochlorite or 0.5% Wescodyne).

Be sure to cover an area twice the size of the spill with disinfectant
5. Allow to sit for at least 20 minutes for inactivation, see agent specific handling
procedures.

If there is broken glass or other items use forceps, tongs, or a broom to remove
and place in the appropriate container (i.e. glass into sharps container, pipettes
into biohazard bags)
6. Remove and dispose of soaked paper towels as biowaste.*
7. Re-clean area with disinfectant solution
8. For spills that are large and require assistance please contact EH&S at 6-3613.
9. PI must complete an Incident Reporting form and submit to the IBC within the provided
time frame.
*Note: bleach soaked paper towels should not be autoclaved. Chlorine is a corrosive and will
corrode the autoclave over time.
Type of spill or exposure
Exposure to a Risk Group 2 or 3 agent
Exposure to r/sNA molecules
Reporting time frame
Report immediately to the Biosafety Officer.
Submit Incident Reporting Form to the IBC
within 5 days of exposure.
6
Spill outside the BSC with a Risk Group 2 or 3
agents
Incident Reporting Form must be submitted
to the IBC within 7 days of incident. If an
exposure has occurred, see above.
Spill outside the BSC with r/sNA molecules
Spill Kits
The lab must have a spill kit, or the components of such, readily accessible in the
event of a spill. The spill kit should include:





an easy-to-read outline of the spill response SOP
gloves
surgical masks
safety glasses or goggles
clean lab coat, disposable gown or clean scrubs and spare slip-on shoes in
case clothing not covered by lab coat becomes contaminated
 paper towels or other products to absorb contaminated liquids
 disinfectant (e.g., 10% bleach)
 tongs or forceps to pick up broken glass
 a biohazard waste container
Medical Treatment Locations
Student Health Services
Hours: Monday- Friday 8:30am -10:30pm
Sunday& Saturday 8:00am- 3:30pm
Hilda May Williams Building
234 Glenbrook Road, Unit 4011
Storrs, CT 06269
860-486-4011
UConn Urgent Care, Storrs Center (Faculty/Staff)
Hours: Monday-Sunday 10:00am- 6:00pm
Storrs Center
One Royce Circle, Suite 103
Mansfield, CT 06268
860-487-9300
Windham Hospital (after hour’s emergencies)
112 Mansfield Ave
Willimantic, CT 06226
860- 456-9116
7
EXAMPLE
Helpful Resources for completing Agent Write-up
 Canadian Pathogen Safety Data Sheets: http://www.phac-aspc.gc.ca/lab-bio/res/psdsftss/index-eng.php
 BMBL, 5th Edition: http://www.cdc.gov/biosafety/publications/bmbl5/
 CDC A-Z Index: http://www.cdc.gov/az/a.html
AGENT: ADENOVIRUS
Risk Group: 2
I.
HEALTH HAZARDS
Adenoviruses are non-enveloped, linear double-stranded DNA viruses. Adenoviral vectors are viruses
that are specifically used to introduce exogenous DNA into host cells. Adenoviral vectors can accept
large gene inserts, can be produced in high titers, and can infect a wide variety of cell types.
Adenoviruses are unusually stable to chemical or physical agents and adverse pH conditions. They are
very stable in the environment and can survive 3 to 8 weeks on environmental surfaces at ambient
temperatures. Adenovirus is a pathogen of respiratory, gastrointestinal mucosa and mucous
membranes. The symptoms of respiratory illness resulting from adenovirus can range from the
common cold to pneumonia, croup, and bronchitis.
Additional clinical symptoms include conjunctivitis (“pink eye”), cystitis, gastroenteritis (stomach flu),
tonsillitis, rash -associated illness, and rare cases of severe disease, especially in those with
compromised immune systems. Unlike HIV and herpes, adenovirus is quite stable. After being
extracted with ether and/or chloroform, it can still be infective. Humans and animals are the natural
reservoirs for wild-type adenoviruses. In addition, potential health hazards may vary depending on the
nature of the gene insert (i.e. toxin or cell growth modifier).
II.
MODES OF TRANSMISSION
Inhalation of aerosolized droplets, mucus membrane, parenteral inoculation, or ingestion
III.





IV.
ADMINISTRATIVE CONTROLS
Access to the laboratory shall be restricted, doors must remain closed during experimentation
Signs incorporating the biohazard symbol must be posted at the entrance of the laboratory where
agent is being used
ALL laboratory personnel must be advised of the hazards of the agent
ALL laboratory personnel must be trained in the proper handling, use and disposal prior to working
with the agent
All laboratory personnel must remove lab coats, discard gloves in the proper biowaste container
and wash hands before exiting the lab.
ENGINEERING CONTROLS

All adenovirus work must be performed in a BSL-2 laboratory and require a Certified Class II BSC

The BSL may be lowered by the IBC when working with adenoviral vectors. This is dependent on
the nature of the inserted genes and its replication competence

NO open-bench work!
8

Biohazard Sharps Containers shall be available to dispose of sharps waste, including broken
glass, needles, blades, etc.

Centrifugation must be performed in closed containers and using sealed rotors or safety cups to
minimize the risk of aerosol generation. Samples must be placed into and/or removed from cups
within a BSC.

Vacuum: all vacuum lines must be fitted with a HEPA filter and a vacuum flask, containing the
appropriate disinfectant in a volume sufficient to provide the recommended final concentration for
that disinfectant when the flask is near full. At the end of the work session, aspirate a small volume
of concentrated disinfectant through the vacuum tubing, into the vacuum flask. The vacuum flask
must sit for a minimum time of 30 minutes prior to drain disposal.

Vortexing: must be done in BSC.

Pipetting: Aerosol resistant (filtered) tips must be used when pipetting.

Sharps (ONE-TIME USE): All sharps should be immediately disposed of in to a sharps container
(located within the BSC).
V.
PERSONAL PROTECTIVE EQUIPTMENT (PPE)
The following PPE must be worn at ALL times when handling Adenovirus
Gloves
Lab Coat
Safety Goggles/Glasses
Shoe Covers
Gown
N95 Respirator: required for aerosol-producing procedures performed oustside a
BSC
Surgical Mask
VI.
Face Shield
Other:
DISINFECTION

Disinfectants: 10% Sodium Hypochlorite (1:10 dilution household bleach, such as Clorox), 2%
Gutaraldehyde, 0.25% Sodium Dodecyl Sulfate

Contact time: 30 minutes before cleanup

Alcohol is NOT an effective disinfectant against adenovirus
VII.
DISPOSAL
Liquid waste may be treated with exposing to bleach (final concentration volume 10%) for 30 minutes
or longer before disposing into the sink.
Solid biohazardous waste, such as culture vials, plates, plastic tubes, etc., are disposed of into an
autoclave bag, autoclaved for 60 minutes at 121°C under 15lbs psi of steam pressure, sealed, and
labeled, and placed in a 2 layered biohazardous box-bag unit for pickup by EH&S.
Sharps waste, such as broken glass, pasteur pipets, razor blades, and needles, are disposed of into
an approved biohazard sharps containers, autoclaved for 60 minutes, placed in a box-bag unit for
pickup by EH&S.
VIII.
PRACTICES FOR ANIMAL INJECTIONS
Animals may shed adenovirus for 72 hours post inoculation and must be housed under ABSL-2
conditions during this period prior to being removed from their containment, if allowed by protocol.
9
Principal Investigators or designee must make arrangements for housing these animals and for
disposing of the contaminated bedding by contacting the appropriate animal care supervisor before
research begins.

Facility: When animals are infected with adenoviral vectors, the Animal Biosafety Level of the
project will be assigned to ABSL-2. This requires Biosafety Level-2 practice and facilities for
procedures involving adenoviral vectors.

Signage: Attached door sign must be posted on the door leading into the housing or procedure
room. Cages must be labeled with the biohazard cage card label with agent identification and
injection date upon injection of agent. Signage/labels must remain in place for a minimum of 1
week after the date of injection/exposure or as long as required in the approved protocol.

Animal Excretion: Infected animals my excrete adenovirus. Precaution must be taken not to
create aerosols when emptying animal waste material and when washing down cages, or cleaning
the room with pressure hoses. Surfaces that may be contaminated will be decontaminated ASAP
with bleach solution. This practice must be followed for one full week after infection or as long as
required in the approved protocol.

Cage Change: Use a Certified Class II Biosafety Cabinet when moving animal from dirty to clean
cages. Spray the inside surfaces of each cage with bleach solution and leave the cages in the
biosafety cabinet for 15 minutes before returning them to the cage washing facility. Decontaminate
the BSC immediately after removing the contaminated cages with bleach solution.

Bedding: A BSC or negative airflow cage changing station should be used when disposing
bedding into biohazard red bag. The red biohazard bag must be sealed and placed into the
biohazard container.

Animal Carcass: Infected carcasses should be placed in red biohazard bag and stored in
designated freezer for EH&S pick-up. EH&S must be informed when carcasses are put into
freezer.
IX. SPILL AND EXPOSURE PROCEDURES
See pages 5-7 for guidance
X.
MEDICAL SURVEILLANCE
All laboratory personnel must be advised by the PI on the health hazards of adenovirus prior to
starting work within the laboratory. If a potential exposure may have occurred after seeking medical
attention symptoms must be monitored and/or confirm by serology.
XI.
FIRST AID/ TREATMENT
Mainly supportive; no vaccines presently available for Ad5
Agent: Adenovirus Training Record
I have reviewed and understand the risks associated with the Adenovirus. I understand that my
signature below indicates I agree to comply and work safely with the said agent.
Lab Personnel
Training Date
Signature
Example: Wallace Rowe
January 1, 2015
Wallace Rowe
10
AGENT: ENTER NAME
COPY AND PASTE THIS AGENT RISK ASSESSMENT TEMPLATE AS
NEEDED
Risk Group: ENTER RISK GROUP
I.
HEALTH HAZARDS
[Include general description of hazards posed by infectious agent, which may include
characteristics of the agent, diseases or symptoms it may cause, and the major risk factors for
infection within the laboratory. Helpful Resources for completing Biohazardous Material Writeup
•
Canadian Pathogen Safety Data Sheets: http://www.phac-aspc.gc.ca/lab-bio/res/psdsftss/index-eng.php
•
BMBL, 5th Edition: http://www.cdc.gov/biosafety/publications/bmbl5/
•
CDC A-Z Index: http://www.cdc.gov/az/a.html]
ENTER HERE
II.
MODES OF TRANSMISSION
Include likely modes of transmission in the laboratory, such as direct skin, eye, or mucosal
membrane exposure, parenteral inoculation by needle or other contaminated sharp, ingestion of
liquid suspension or contaminated hand to mouth exposure, or inhalation of aerosols. This
information may also be found in the SDS or CDC Guidelines, as stated above.
ENTER HERE
III.
ADMINISTRATIVE CONTROLS
Describe any additional work practices used to limit potential exposures, examples have been
provided.
ENTER HERE





IV.
Access to the laboratory shall be restricted, doors must remain closed during experimentation
Signs incorporating the biohazard symbol must be posted at the entrance of the laboratory where
agent is being used
ALL laboratory personnel must be advised of the hazards of the agent
ALL laboratory personnel must be trained in the proper handling, use and disposal prior to working
with the agent
All laboratory personnel must remove lab coats, discard gloves in the proper biowaste container
and wash hands before exiting the lab.
ENGINEERING CONTROLS
Describe the controls used to reduce exposure in the lab, examples have been provided

All ENTER AGENT OR TOXIN NAME work must be performed in a BSL-2 laboratory and
require a Certified Class II BSC

NO open-bench work!

Biohazard Sharps Containers shall be available to dispose of sharps waste, including broken
glass, needles, blades, etc.
11

Centrifugation must be performed in closed containers and using sealed rotors or safety cups to
minimize the risk of aerosol generation. Samples must be placed into and/or removed from cups
within a BSC.

Vacuum: all vacuum lines must be fitted with a HEPA filter and a vacuum flask, containing the
appropriate disinfectant in a volume sufficient to provide the recommended final concentration for
that disinfectant when the flask is near full. At the end of the work session, aspirate a small volume
of concentrated disinfectant through the vacuum tubing, into the vacuum flask. The vacuum flask
must sit for a minimum time of 30 minutes prior to drain disposal.

Vortexing: must be done in BSC.

Pipetting: Aerosol resistant (filtered) tips must be used when pipetting.

Sharps (ONE-TIME USE): All sharps should be immediately disposed of in to a sharps container
(located within the BSC).
V.
PERSONAL PROTECTIVE EQUIPTMENT (PPE)
Describe the PPE to be worn when working with agent or toxin.
The following PPE must be worn at ALL times when handling ENTER AGENT OR TOXIN NAME
Gloves
Lab Coat
Safety Goggles/Glasses
Shoe Covers
Gown
N95 Respirator: ENTER SPECIAL COMMENTS OR DELETE INSTRUCTIONS
Surgical Mask
VI.
Face Shield
Other:
DISINFECTION
Add any additional methods of disinfecting the agent. General surface disinfection has been
provided.
ENTER HERE


VII.
Disinfectants: 10% Sodium Hypochlorite (1:10 dilution household bleach, such as Clorox)
Contact time: 30 minutes before cleanup
DISPOSAL
Describe any additional methods on how the agent/toxin will be disposed; standard methods
are listed below
ENTER HERE
Liquid waste may be treated with exposing to bleach (final concentration volume 10%) for 30 minutes
or longer before disposing into the sink.
Solid biohazardous waste, such as culture vials, plates, plastic tubes, etc., are disposed of into an
autoclave bag, autoclaved for 60 minutes at 121°C under 15lbs psi of steam pressure, sealed, and
labeled, and placed in a 2 layered biohazardous box-bag unit for pickup by EH&S.
12
Sharps waste, such as broken glass, pasteur pipets, razor blades, and needles, are disposed of into
an approved biohazard sharps containers, autoclaved for 60 minutes, placed in a box-bag unit for
pickup by EH&S.
VIII.
PRACTICES FOR ANIMAL INJECTIONS IF APPLICABLE
Describe any additional special practices for handling animals exposed to agent/toxin
Facility: ENTER HERE
Signage: ENTER HERE
Animal Excretion: ENTER HERE
Cage Change: ENTER HERE
Bedding: ENTER HERE
Animal Carcass: ENTER HERE
IX.
SPILL AND EXPOSURE PROCEDURES
See pages 5-7
X.
MEDICAL SURVEILLANCE
All laboratory personnel must be advised by the PI on the health hazards of the ENTER AGENT OR
TOXIN NAME prior to starting work within the laboratory. If a potential exposure may have occurred
after seeking medical attention symptoms must be monitored and/or confirm by serology.
XI.
FIRST AID/ TREATMENT
[Describe any first aid/ treatment for exposure to agent/toxin]
ENTER HERE
Agent: ENTER AGENT OR TOXIN NAME Training Record
I have reviewed and understand the risks associated working with ENTER AGENT OR
TOXIN NAME. I understand that my signature below indicates I agree to comply and
work safely with the said agent.
Lab Personnel
Training Date
Signature
13
Safety SOPs for the ENTER PI’S NAME Laboratory when using BSL-2 Agents and
Toxins
The purpose of this section is to develop SOPs that specifically outline instances during
protocols where consideration for safety with a BSL-2 agent/toxin is paramount. Detailed, stepby-step protocols describing entire experiments with materials and methods are not necessary.
Examples of SOPs where safety is emphasized are bulleted below:







Propagation of viruses
Experiments that require PPE in addition to a lab coat and gloves
Experiments that require manipulation of a BSL-2 agent/toxin outside a biosafety cabinet
How to properly vortex or sonicate a viable BSL-2 agent/toxin
Safety concerning the handling of human or non-human primate primary cell lines or tissues
Safety when injecting a research animal with a BSL-2 agent/toxin
How to safely centrifuge a sample containing BSL-2 agents/toxins
Please enter Safety SOPs under separate headings. If SOPS have already been developed,
please insert after this page.
14
STORRS CONTACTS
UConn PUBLIC SAFETY
Emergency
Police Department
Fire Department
911
860-486-4800
860-486-4925
Local Medical Care Facilities
Student Health Services,
860-486-4700
Hours: Monday- Friday 8:30am -10:30pm
Sunday& Saturday 8:00am- 3:30pm
Hilda May Williams Building
234 Glenbrook Road, Unit 4011
Storrs, CT 06269
RESEARCH COMPLINANCE SERVICES Continued
Christine Malloy, J.D.
860-486-9428 office
IRB Expedited Review Coordinator
IACUC Post Approval Monitor
Tekechia Hester, MSc
IBC Program Specialist
IBC Post Approval Monitor
860-486-1838 office
486-1106 fax
Karen Moré
Senior IACUC Coordinator
860-486-2459 office
Arlene Jacobsen
IACUC Coordinator
860-486-4110 office
UConn Urgent Care, Storrs Center,
860-487-9300
Hours: Monday-Sunday 10:00am- 6:00pm
Storrs Center
One Royce Circle, Suite 103
Mansfield, CT 06268
ENVIRONMENTAL HEALTH & SAFETY
Administrative Office
Amy Courchesne
Radiation Safety Manager
860-486-5399
Windham Hospital (after hours emergencies)
112 Mansfield Ave
Willimantic, CT 06226
Terri Dominguez
860-486-0981
RESEARCH COMPLINANCE SERVICES
Nancy Wallach, BA, CIP
Assistant Vice President for Research
860-486-3613
860- 456-9116
Occupational Safety Manager
860-486-4164 office
Douglas Bradway, MA, CIP
IRB Program Associate
860-486-0986 office
Joan Levine, MPH
IRB Post Approval Monitor
860-486-7145 office
Dana Howard,
IRB/ SCRO Administrator
860-486-8802 office
Stefan Wawzyniecki
Chemical Safety Manager
860-486-1110
David Cavallaro
Biological Safety Manager
860-486-3180
15
Appendix II: Spill Response Cue Cards
Cut out cue cards and post in a visible work area
SPILLS INSIDE THE BIOSAFETY CABINET
1. Make sure the cabinet continues to operate. Wait 5 min. to allow aerosols to be
pulled through the HEPA filter.
2. Decontaminate the surfaces within the cabinet wearing protective clothing. Gently
cover the spill with absorbent paper towels and apply the appropriate disinfectant
starting at the perimeter and working towards the center.
* Note: Examine drain pan for contents of the spill. Disinfect if needed.
3. Discard soaked paper towels in a biohazard bag. Wipe up residual fluids. Wipe down
surfaces with 70% EtOH, discarding towels in a biohazard bag.
SPILLS OUTSIDE THE BSC
1. Alert personnel in the vicinity.
2. Check for contaminated clothing, including shoes. Decontaminate if necessary.
3. Evacuate the room. Close door. Discard potentially contaminated PPE and remove
any contaminated clothing. Wash hands thoroughly.
4. Post warning sign: “DO NOT ENTER: Biological spill!”
5. Wait 30 min. Meanwhile, notify PI and a Biosafety Officer/Specialist (6-3180).
6. If assistance is needed, discuss with Biosafety Officer.
7. Don fresh PPE: lab coat or gown, gloves, mask, eye protection.
8. Re-enter the room, cover spill with paper towels.
9. Soak paper towels with appropriate disinfectant, from perimeter toward the center.
10. Allow 30 min. of contact time. Work can continue during contact time.
11. Discarded towels go in biohazard bags. Pick up sharps with tongs & place in sharps
container.
12. Wipe down spill area one final time with appropriate disinfectant.
13. PI, write up a report and submit to the IBC.
17
SPILLS INSIDE AN INCUBATOR
Decontaminate water pan via autoclave.
1. Alert personnel in the vicinity.
2. Evacuate the room. Close door. Discard potentially contaminated PPE and remove
any contaminated clothing. Wash hands thoroughly.
3. Notify PI.
4. Don fresh PPE: lab coat or gown, gloves, mask, eye protection.
5. Cover spill with paper towels.
6. Soak paper towels with appropriate disinfectant, from perimeter toward the center.
7. Allow 30 min. of contact time.
8. Discarded towels go in biohazard bags. Pick up sharps with tongs & place in sharps
container.
9. Wipe down spill area one final time with appropriate disinfectant.
SPILLS INSIDE A CENTRIFUGE
1. Open lid of centrifuge slowly.
2. If there has been no breach of containment, spray rotor with appropriate
disinfectant (e.g. 10% bleach) followed by 70% EtOH to prevent corrosion. See
step #8 next
3. If inside of rotor is contaminated, decontaminate in the BSC. As a precautionary
measure, decontaminate the centrifuge chamber.
4. If rotor buckets are damaged, close centrifuge lid.
5. Alert personnel in the vicinity. Evacuate room.
7. Wait 30 min. Meanwhile, notify PI and a Biosafety Officer/Specialist (6-3180).
If assistance is needed, discuss with Biosafety Officer.
8. Open lid slowly and add paper towels.
9. Spray walls of chamber and rotor with appropriate disinfectant (e.g.10% bleach).
10. Close centrifuge lid for 30 min. of contact time.
11. Finish centrifuge clean-up as for major spill outside the BSC. Transport rotor to
BSC.
12. Open and decontaminate rotor/buckets in the BSC.
13. With PI, write up a report and submit to IBC
18
Appendix III:
Training Certificates/ Email Completion Message
Following this page, please insert copies of training certificates for each person who has completed a training
course listed in the table on page 3.
19
Appendix IV:
Approved IBC Registration and Approval Letter
Following this page, please insert a copy of the IBC approved registration. Also include a copy of the IBC Approval
Letter, recently approved amendments, etc.
Please note: All work with your RG2 agent(s) and r/sNA materials must be pre-approved by the IBC before experiments
can begin.
20
Appendix V:
Recent Laboratory Inspection Reports
Following this page, please insert copies of most recent Post Approval Monitoring and Biosafety Laboratory
Inspection Reports.
21
Appendix VI:
Corrective Action Form
Following this page, please insert copies of your Corrective Action Forms.
22
Appendix VII:
Incident Reporting Forms
Following this page, please insert copies of Incident Reporting Forms submitted to the IBC.
23
APPENDIX VIII
Authorized Personnel Only
BIOHAZARDOUS SPILL
DO NOT ENTER!
Biosafety Level 2
Biological Agents:
Special Procedures,
PPE, or Precautions for
Entry/Exit:
Name
Principal Investigator
Phone
Emergency Contact (must be 24/7)
Name
Phone
24