BIOMED 3200
Comparative Hematology
COURSE PROFILE
Course Description
This course of Comparative Hematology is designed to hone the
skills of the practicing Veterinary Technician and assumes some
basic knowledge of erythrocytes, leukocytes and platelets. The
review of normal will be minimal and emphasis will be placed on
changes associated with disease. In addition, this course will
explore issues in transfusion medicine and the diagnosis of
coagulation disorders.
Instructor(s)
Linda M. Berent, DVM, PhD, Diplomate ACVP (Clinical and
Anatomic Pathology)
A344 Clydesdale Hall
College of Veterinary Medicine
Office Phone: 882-0086
e-mail: BerentL@missouri.edu
Natalie C. Hoepp, LVT, DVM
B204 Clydesdale Hall
College of Veterinary Medicine
e-mail: Hoeppn@missouri.edu
Major Objectives
After completion of the course, a student will be able to:
1. Understand the common cell counting techniques and their
limitations
2. Be able to establish and maintain a quality control program for
an in house laboratory.
3. Perform a slide review and manual differential with confidence
on abnormal samples.
4. Be able to evaluate platelet and coagulation disorders
5. Understand the basics of blood groups and transfusion
medicine.
6. Have a working knowledge of avian, reptile and pocket pet
hematology when if differs from typical companion and food
animals.
Prerequisites
Delivery
An AAS or equivalent degree in veterinary technology from an
American Veterinary Medical Association-accredited program.
This course is delivered completely online. Students are not
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BIOMED 3200– Comparative Hematology
Syllabus and Schedule - Page 1
required to attend class at regular times; however, it is important
that they follow the attendance/participation guidelines and meet
due dates and deadlines for readings, assignments, discussions,
quizzes, and exams. Communications will be through the
discussion board, announcements, and emails. Real time video
conferencing is possible on request.
Course delivery strategies may include: reading from required
textbook(s), Aperio Digital Microscopy slides, reading resources
linked to the internet, brief audio or audio/video lectures, assigned
projects, use of the discussion board, use of the internet, and e-mails.
Organization
Course materials are located under the left-hand tab in the course
Blackboard site. Further directions are provided in Blackboard.
Required Materials
Clinical Pathology for the Veterinary Team. Wiley-Blackwell
2010.
EVALUATION OF
STUDENT PERFORMANCE
Satisfactory
Performance
There will be 1000 possible points. A portion of the possible
points will be achieved each week. More than 600 is required for a
passing grade. Two proctored exams will provide a total of 400
points. A cell image portfolio assignment worth 300 points will
be due by the end of the semester. The remaining 300 will be
based on weekly quiz scores, assignments, professionalism
(attendance, meeting deadlines), and contributing to required
course blogs. The instructor reserves the authority to adjust final
grades if no student achieves 100% of possible points in the
course. Students with less than 61% at mid-term will receive a
warning. Grades will be available in Blackboard. To insure
confidentiality, they will not be sent by e-mail.
Students must log on to the course at least twice each week to
receive professionalism points. Students who perform well
typically log on 3 or more times per week.
Exams and Quizzes
Exams are available only under the supervision of a proctor.
Contact Mizzou Online to locate a local proctor for your exams.
Quizzes are not proctored, but you are expected to complete the
quizzes by yourself. With the exception of the syllabus quiz, each
quiz is timed so that you will not have time to rely on reference
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BIOMED 3200– Comparative Hematology
Syllabus and Schedule - Page 2
materials, i.e. they are not open-book quizzes.
If you take more time than the quiz or exam allows, your score will
be deducted the points of one question each minute in overtime.
You may take a quiz or exam only once. You must complete the
exam or quiz once you start it. You may NOT come back to the
quiz later. If you are disconnected during an exam, contact the
instructor immediately and then send an e-mail to
blackboard@missouri.edu with your name, username, course
name, title of the quiz or exam, and a description of the problem.
To ensure your answers are logged, click “Save” at the bottom of
the page every 2 to 3 questions. Click “Submit” after you have
reviewed your answers to have the quiz or exam graded.
Scoring of Assignments
In addition to exams and quizzes, students will be scored on course
participation by submitting blogs and answering discussion
questions. Blogs and discussion questions will be scored on
thoughtful content, appropriate length, grammar, and spelling.
Grading
The grading scale will be A to F, including some pluses and
minuses but no A+, C+, C-, D+, or D-. Grades will be based on
the following scale:
96-100% =
91-95% =
88-90% =
84-87% =
81-83% =
71-80% =
61-70% =
60% or less =
A
AB+
B
BC
D
F
Note: A Certificate in Biomedical Technology or Registry of
Approved Continuing Education (RACE) CE credit requires at
least a C- grade in this course, plus a total of 15 cr hr BIOMED
courses with an average GPA in all BIOMED courses of 3.0.
Points used in grading this course:
Week
Exam
Quiz/Assignment
Blog/Discussion
Running Total
1
---
10
10
20
2
---
---
10
30
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BIOMED 3200 – Veterinary Hematology
Syllabus and Schedule - Page 3
3
---
10
10
50
4
---
20
10
80
5
---
10
10
100
6
---
20
7
---
40
8
200
9
10
120
170
---
10
---
---
---
10
380
---
20
10
410
11
---
---
10
420
12
---
20
10
450
13
---
10
10
470
14
---
20
10
500
15
200
300
---
1000
370
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BIOMED 3200 – Veterinary Hematology
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COURSE
SCHEDULE
Date
Topics
Reading
Assignments*
Week 1
i. Syllabus Quiz, black board
introduction
ii. Manual, impedance and flow
cytometry
iii. Calculated vs measured values,
sample quality
i. PCV, T.P., hemoglobin – methods and
interferences
ii. Common red cell breed and age
differences
iii. Introduction to Aperio slides, review
of species normal
a. Syllabus
b. Chapter 2
c. Instrument
Tegrity ppts
1-3
Week 2
Points Assignments
a. Syllabus Quiz (10 pt)
b. Discussion board (10 pt)
a. Chapter 2
a. Discussion board (10 pt)
b. RBC Tegrity
ppt 1
c. Aperio
tutorial
Tegrity
a. Post on image Blog (10
a. Chapter 4
pt)
b. RBC Tegrity
b. Aperio practice image
ppt 2
assignment due (10 pt)
Week 3
i. Poikilocytosis - artifact vs significant,
Hemoparasites,
ii. Regenerative and non regenerative
Anemias, Polycythemia, relative vs
absolute
iii. Red cell maturation and bone marrow
responses
Week 4
i. Quality Control
ii. S.O.P introduction
a. ASVCP QC
guidelines
b. Chapter 1
Week 5
i. Review of normal leukocyte
morphology and breed differences
ii. Morphologic abnormalities and
parasites
Week 6
i. Relative vs Absolute values
ii. Leukocyte changes in disease
iii. Species and age differences
a. Chapter 4
a. Turn in Draft of SOP
b. WBC Tegrity
assignment (10pt)
ppt 1
b. Post on image Blog (10
pt)
a. Chapter 4
a. Complete critiques of
b. WBC Tegrity
SOP (20 pt)
ppt 2
a. Red cell quiz (20 pt)
b. Discussion board (10 pt)
a. Avian and
a. Leukocyte Quiz (20 pt)
Reptile
b. Turn in final draft of
Tegrity ppt
SOP (20 pt)
b. Website links c. Post on image Blog (10
pt)
Pocket pet and other species –
a. Exotic
Week 8
a. Exam #1 (200 pt)
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Week 7
Avian and Reptile – differences from
typical companion and food animals.
BIOMED 3200 – Veterinary Hematology
Syllabus and Schedule - Page 5
differences from typical companion
and food animals.
Tegrity ppt
Week 9
Leukemia
i. Acute vs chronic – classic patterns
ii. myeloid vs lymphoid vs erythroid –
cell markers
iii. Lymphoma vs leukemia
a. Leukemia ppt a. Discussion board (10 pt)
Week 10
Bone marrow evaluation
i. Sample Quality, collection, aspirates
vs cores
ii. CBC correlates and Concurrent viral
diseases – effects on marrow
a. Leukemia, bone
a. Chapter 3
marrow quiz (20 pt)
b. Bone marrow
Tegrity ppt. b. Post on image Blog (10
pt)
Week 11
Platelets
i. Counting methods and bone marrow
elements
ii. Response to disease and clinical signs,
breed differences
Inherited and acquired thrombocytopathy
and testing
Coagulation testing
i. ACT, PT, PTT testing
ii. Inherited and acquired disorders
iii. coagulation, platelet quiz
a. Chapter 13
a. Discussion board (10 pt)
a. Chapter 13
a. Coagulation Testing
Quiz (20 pt)
b. Post on image Blog (10
pt)
Week 13
i. Cross matching and blood groups
ii. Blood Donors
Week 14
iii. Neonatal Isoerythrolysis
iv. Blood Products
a. Transfusion a. Blood groups
assignment due (10 pt)
Tegrity ppt 1
b. Discussion board (10 pt)
a. Transfusion a. Discussion board (10 pt)
Tegrity ppt 2 b. Transfusion quiz (20 pt)
Week 15
Review
Week 12
a. Exam #2 (200 pt)
Estimated Time Requirements for Preparation and Participation:
Three clock hours per Module – or 18 hours per Session (week) – or – 126 hours for the course
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