Faculty of Medicine and Health
School of Medicine
Leeds Institute of Biomedical & Clinical Sciences
Section of Ophthalmology and Neuroscience
Research Fellow
100% of full time basis
Based at St James’s University Hospital
This post is fixed term for 2 years and is available from 1st September 2015
Applications are invited for a Research Fellow post to be based within the group of Professor Colin
Johnson studying the genetics and molecular cell biology of ciliopathies. Ciliopathies are a class of
human developmental conditions that are of increasing relevance to basic research and clinical
practice. The post is funded by a Medical Research Council project grant and will contribute to the
execution, analysis and interpretation of siRNA-based reverse genetics screens for novel ciliopathy
genes and processes.
The siRNA screens will be performed in collaboration with the BioScreening Technology Group
(BSTG) at the University of Leeds. The project will require an excellent knowledge of modern
techniques of genetics (including next generation sequencing methodologies) and advanced
imaging techniques (including confocal microscopy and high-content imaging). You will also
evaluate screen hits using functional assays, including the assessment of protein-protein
interactions using affinity purification methods (TAP-tagging), so previous experience of these
systems is desirable. A good knowledge of biochemical assays is essential.
Candidates must be enthusiastic, highly motivated and demonstrate a high level of relevant
laboratory experience and technical competence. In addition, the post holder must show the ability
to manage their time effectively and work under pressure, exhibiting effective interpersonal,
communication, and team-working skills. You must have a PhD in molecular/cell biology or a
relevant biomedical subject (or have a date set for examination) and an interest in medical
genetics. A background in human molecular genetics or developmental biology is desirable.
The University of Leeds is committed to providing equal opportunities for all and offers a
range of family friendly policies (http://hr.leeds.ac.uk/homepage/4/policies). The University is a
charter member of Athena SWAN and holds the Bronze award. The School of Medicine
gained the Bronze award in 2013. We are committed to being an inclusive medical school
that values all staff, and we are happy to consider job share applications and requests for
flexible working arrangements from our employees.
University Grade 7 (£31,342 – £37,394 p.a) depending upon qualifications and experience
Informal enquiries regarding the post should be directed to Prof. Colin A. Johnson, email:
c.johnson@leeds.ac.uk , tel +44 (0)113 343 8443
If you have any specific enquiries about your online application please contact Colette Cornelly,
email c.l.cornelly@leeds.ac.uk tel +44 (0)113 206 9229 or Sharon Collins, email
s.collins@leeds.ac.uk or tel +44 (0) 113 206 6949.
Job Ref: MHBCS1014
Closing Date: 22 September 2015
Job Description
Job Summary
The project will require transfection studies with epitope-tagged constructs and siRNA
oligonucleotides, biochemical assays of protein-protein interactions, high content image analysis of
a reverse genetics screen and the anatomical and functional characterization of mouse models of
human ciliopathies. The project will involve cell culture, including derivation of mouse embryonic
fibroblasts, advanced microscopy techniques, recombinant DNA technology, and will have a
significant component of bioinformatic and image analysis.
BioScreening Technology Group (BSTG)
The BioScreening Technology Group (BSTG) provides an interactive service to enable the
characterization of the function and interactions of particular proteins, and support the identification
and validation of potential biomarkers (diagnostic, prognostic or predictive) and therapeutic targets.
More specifically, the Group helps users to develop and formulate plans for the execution of
projects to: (1) develop probes for biomarkers/drug targets and (2) undertake reverse genetic
screens. The BSTG is funded by the Leeds Biomedical and Health Research Centre to promote
collaboration between basic sciences, translational and clinical research. The reverse genetics arm
of the service consist of a manager, postdoctoral fellow and a technician who are all based in the
section of Ophthalmology and Neurosciences.
For background on methodologies, see the following publications:
Elmehdawi F, et al. (2013) Human Homologue of Drosophila Ariadne (HHARI) is a marker of
cellular proliferation associated with nuclear bodies. Exp. Cell Res. 319:161-172.
Logan CV et al. (2011). Mutations in MEGF10, a regulator of satellite cell myogenesis, cause earlyonset myopathy, areflexia, respiratory distress and dysphagia (EMARDD). Nat. Genet. 43:1189–
1192
Valente EM & Logan CV et al. (2010). Mutations in TMEM216, encoding a novel tetraspan protein,
perturb ciliogenesis and cause Joubert (JBTS2), Meckel (MKS2) and related syndromes. Nat.
Genet. 42: 619-625
Main Duties & Responsibilities
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Performing data analysis in collaboration with the BioScreening Technology Group (BSTG)
to determine positive ‘hits’ in reverse genetics screens
Analysing next generation sequencing data to identify pathogenic mutations in ciliopathy
patients
Under the guidance of the principal investigator plan and manage your own research
activity (in collaboration with others as appropriate), assisting in designing specific
strategies towards achieving the research goals of the project and managing individual
components of the study
Ensuring good day-to-day progress of the projects and contributing to strategic
development
Designing experimental procedures and technical details
Molecular and cell biology techniques to be used will include:
o DNA/RNA extraction
o PCR for microsatellite genotyping and direct Sanger sequencing
o preparation of libraries for whole exome sequencing
o mammalian cell culture
o transfection with plasmids or siRNA/shRNA
o RT-PCR and quantitative (‘real-time’) RT-PCR
o biochemical assays of protein-protein interactions using affinity purification methods
(TAP-tagging)
o confocal microscopy and live-cell imaging
o high content imaging
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o bioinformatic analyses of data-sets
Generating research output (e.g. publications/presentations) and analysing and interpreting
the results
Communicating and/or presenting research results within the Group and Section
Developing independent and original ideas, as appropriate
Continually evaluating your working practices and protocols and adapting/evolving these
where necessary
Troubleshooting problems with optimisation, assay design, screening, data analysis and
validation and seeking solutions via published literature, company websites and personal
contacts.
Undergoing training in high content high throughput microscopy in order to perform, image
capture and data analyse
Maintaining accurate and up-to-date laboratory and other records
Treating data with confidentiality and in accordance with current policies
Building internal contacts and participating in internal networks for the exchange of
information and for future collaboration
Working collaboratively with other staff/students within the Group, BSTG, OPNE and other
research groups as appropriate, including external collaborators
Actively engaging with the regional human molecular genetics community, ensuring
research outputs are shared via existing networks and providing specialist knowledge to
others
Encouraging and promoting good working practices within the team and within the Institute
Providing guidance, support, training and assistance in relevant techniques to staff and
students within the Group and Section
Publishing outcomes from the project in appropriate high quality journals and presenting at
national or international conferences as required
Working with the Principle Investigator to identify future opportunities for research and
preparing proposals and funding applications
Attending and contributing to relevant meetings
Being aware of the risks in the laboratory environment and working in accordance with the
GM and COSSH assessments
Contributing to GM, RA and COSSH assessments when appropriate.
Actively supporting and adhering to the Faculty’s commitment to Equality and Diversity in
line with the requirements of the Faculty Diversity Action Plan
Please note, you may be required to work flexibly outside of normal working hours according to the
needs of the project.
Job descriptions cannot be exhaustive and you may be required to undertake other duties that are
broadly in line with the above key responsibilities.
Relationships
The post holder will be responsible on a day-to-day basis to the Principal Investigator, Prof. Colin
A. Johnson, through whom s/he will be accountable to the Head of Section (Prof. Chris
Inglehearn), the Director of the Institute and to the Dean of the Faculty of Medicine & Health. S/he
will be expected to interact with other members of the Section of Ophthalmology and
Neurosciences, comprising post-doctoral research fellows, PhD students and research support
staff, and to present data at Section and Institute meetings.
University Values
All staff are expected to operate in line with the university’s values and standards, which work as
an integral part of our strategy and set out the principles of how we work together. More
information about the university’s strategy and values is available at
http://www.leeds.ac.uk/comms/strategy/
Person Specification
Essential
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A PhD in molecular/cell biology or a relevant biomedical subject (or pending with a date
set for examination)
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A BSc degree (or equivalent) in human molecular genetics, developmental biology or
related subject
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A demonstrable interest in medical genetics and/or developmental biology
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Extensive experience in biochemical assays of protein-protein interactions
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Extensive experience in cell culture, including culturing primary cells
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Extensive experience in siRNA techniques
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Practical experience of immunofluoresence microscopy
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A proven track record in molecular genetics, including experience of RT-PCR and
quantitative (real time)-PCR, experience in molecular cloning techniques and generation of
vectors
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A proven track record of direct sequencing, including whole exome sequencing,
preparative and analysis methodologies
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A demonstrative ability to analyse and interpret research results
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A demonstrable ability to work unsupervised, with the direction from the Principle
Investigator and as part of the wider research team
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Effective communication and interpersonal skills
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A developing publication record in high impact peer reviewed journals
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A demonstrable understanding of health and safety issues within the laboratory setting
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A demonstrable understanding of translational research
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Strong IT skills, including the use of Microsoft Office and image processing applications
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A willingness to work flexibly, when necessary, to fulfil the needs of the research project
Desirable
 A demonstrable research background in human molecular genetics or developmental
biology (or a familiarity with their concepts)
 A demonstrable research background in high-throughput screening methods
 Demonstrable experience in mouse embryonic stem cell culture
Further Information
Faculty Information
With more than 6,000 students, 1,600 staff and annual research income of £50m, the Faculty of
Medicine and Health at Leeds is bigger than some universities. Leeds has one of the largest
medical and bioscience research bases in the UK, and is an acknowledged world leader in cancer,
cardiovascular, psychiatric, genetic, musculo-skeletal and health services research. Treatments
developed in Leeds are transforming the lives of people around the world living with conditions
such as HIV, TB, diabetes and malaria.
The School of Medicine
The School of Medicine at the University of Leeds is a major international centre for research and
education. Our ambition is to improve health and reduce health inequalities, locally and globally,
through excellent scientific research and the translation of that research into healthcare practice,
and through the education of future scientific and clinical leaders who will advocate and practise an
evidence-based approach. Our major strategic aims are to:
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Deliver outstanding research including basic discovery science through to applied health
research that makes a significant difference to health.
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Produce exceptional graduates, clinicians, educators, doctoral and post-doctoral fellows
whose learning has been informed and inspired by our research excellence and who will
form the next generation of academic and clinical leaders.
Develop and support knowledge transfer activities that flow from our academic activities.
Create and maintain an efficient and sustainable environment for research and teaching
within an organisational culture and management style that enacts and supports the
university’s core values of community, inclusiveness, integrity and professionalism.
The School of Medicine is organised into seven Institutes. All are committed to high quality
research-led teaching, through their training of postgraduate research students, delivery of
postgraduate taught courses, and its leadership in undergraduate teaching. The School works
closely with the local NHS, having a number of jointly funded clinical posts to ensure this
relationship is effective and strong for both research and student education.
Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM) Director: Professor
Mark Kearney
LICAMM integrates basic and clinical scientists with a common goal of understanding the
mechanisms underpinning common chronic diseases of human health and developing new
approaches to treating patients at an individual and population level. At the heart of LICAMM’s
philosophy is a vibrant multidisciplinary approach to science that provides a platform to deliver
internationally competitive translational research and teaching in disorders including cardiovascular
disease, diabetes, cancer and neurodegenerative diseases) our key aim is to improve the lives of
our patients and the experience of our students.
Leeds Institute of Health Sciences (LIHS) Director: Professor Tim Ensor
LIHS delivers problem-driven research that supports decisions about the content or delivery of
healthcare. Our interdisciplinary approach incorporates expertise in applied health research
designs, health implementation sciences, social sciences, health economics, informatics and
statistics, as well as skills in communicating with basic scientists, policy makers, healthcare
providers, public and patients. We conduct research at the individual, population and
organisational level.
Leeds Institute of Medical Education (LIME) Director: Professor Trudie Roberts
LIME provides the administrative support, co-ordination and leadership for the School of
Medicine’s undergraduate medical degree, including admissions, curriculum development,
assessment, student support and clinical placement liaison. It provides the technology-enhanced
learning and innovation support for the School of Medicine. LIME also has a very active
scholarship programme of research and innovation in medical education and uses its expertise to
influence medical education policy and practice nationally and internationally. To achieve this it
works with a range of stakeholders including the academic community, the profession, the public,
regulators and policy makers.
The Leeds Institute of Cancer and Pathology (LICAP)
Director: Professor Tim Bishop
The Leeds Institute of Cancer and Pathology addresses both laboratory based and clinical
research into cancer with a major focus on translational science. LICAP is one of the largest
cancer Institute’s in the country and has major financial support from the cancer charities. The
laboratories and clinical research are all based on the St James’s site with laboratory activities
being located in the Wellcome Trust Brenner Building and adjacent buildings while the clinical work
is based within Bexley Wing. The Institute consists of seven Sections: Epidemiology &
Biostatistics; Experimental Haematology; Experimental Oncology; Oncology & Clinical Research
(Clinical); Oncology & Clinical Research (Laboratory); Patient Centred Outcome Research; and
Pathology & Tumour Biology.
Leeds Institute of Biomedical & Clinical Sciences (LIBACS) Director: Professor Philip
Hopkins
LIBACS undertakes clinically-driven research from the level of the gene through cellular, tissue and
organ to clinical trials. Our vision is to develop a sustainable centre of excellence for the
advancement of patient care by translating research results into clinical practise and contributing to
medical education at undergraduate and postgraduate levels. Our research interests are
encapsulated in 6 clinical themes (Gastrointestinal inflammation & tumorigenesis, Genetic
disorders, Infection & immunity, Neuroscience, Perinatal medicine, Perioperative outcomes &
technologies) underpinned by 4 generic science technology strands (Animal models, Cell biology,
Gene regulations & Genomics). We are based predominantly at the St James’s University Hospital
site.
Leeds Institute of Rheumatic and Musculoskeletal Medicine (LIRMM), Director: Professor
Paul Emery
LIRMM is dedicated to improving diagnosis, therapy, intervention and outcome across the
spectrum of rheumatic and musculoskeletal medicine. It boasts a dynamic portfolio of research and
education, delivering wide-ranging clinical, translational and basic research across five Sections:
Clinical Musculoskeletal Medicine, Experimental Musculoskeletal Medicine, Clinical Biomechanics
and Physical Medicine, Rehabilitation Medicine and Orthopaedics. A multi-disciplinary approach is
the core of our activities, with significant interdisciplinary links between Experimental and Clinical
research. LIRMM’s clinical activities are focussed at Chapel Allerton Hospital, which is also base
for our NIHR Musculoskeletal Biomedical Research Unit (LMBRU) and our basic sciences at St
James’s University Hospital.
Leeds Institute of Clinical Trials Research (LICTR) Director: Professor Julia Brown
LICTR delivers innovative design, delivery and knowledge transfer in clinical trials research. Our
multidisciplinary approach, in collaboration with basic scientists, clinicians, policy makers,
healthcare providers, public and patients and University colleagues, delivers internationally
competitive research and teaching that makes a significant contribution to the evidence base for
healthcare delivery. The Institutes research is conducted through the Clinical Trials Research Unit
where we have expertise in design and conduct of complex clinical trials incorporating novel
designs to evaluate CTIMPs, complex interventions, diagnostics, medical devices and surgery.
St James’s University Hospital Campus Infrastructure and Facilities (SCIF) Director:
Professor Pam Jones
This group covers activities that cover School of Medicine functions for Institutes at St James’s
University Hospital that span more than one institute including biomedical research facilities,
student education, IT, health and safety, estates, seminars, PGR studentships and business
support functions. These functions help support the 5 adjacent buildings on the site.
There are 3 Institutes with staff and students at St James’s: LICAP (Leeds Institute of Cancer
studies and Pathology), LIBACS (Leeds Institute of Biomedical and Clinical Sciences), LIRMM
(Leeds Institute of Rheumatic and Musculoskeletal Medicine). These three institutes are dedicated
to basic, translational, clinical and health research integrated with student education.
Additional Information
Terms and Conditions
Details of the terms and conditions of employment for all staff at the university, including
information on pensions and benefits, are available on the Human Resources web pages
accessible via the links on the right hand side, or at http://hr.leeds.ac.uk/policies
University Teaching and Research Award
The Senate of the University has agreed that all newly appointed staff with a contract of 0.5 FTE
and above who have a teaching/ research role and are deemed new to teaching or research in
Higher Education should be required to complete successfully all of the requirements of the
University of Leeds Teaching and Research or an appropriate alternative. Whether or not this
applies to you, will be decided as part of the appointment procedure at interview.
Further details of the ULTRA are available at http://www.sddu.leeds.ac.uk/sddu-ultra.html
Staff with contracts of less than 0.5 FTE may take the ULTRA provided that they have a broad
enough range of teaching, research and assessment to complete the requirements of the
Programme: this will be decided in conjunction with the School and the course providers.
Disclosure and Barring Service checks
A Disclosure and Barring Service (DBS) Check is not required for this position. However,
applicants who have unspent convictions must indicate this in the ‘other personal details’ section of
the application form and send details to the Recruitment Officer
Disabled Applicants
The post is located in the Wellcome Trust Brenner Building in the St James’s University site.
Disabled applicants wishing to review access to the building are invited to contact the department
direct. Additional information may be sought from the Recruitment Officer, email
disclosure@leeds.ac.uk or tel + 44 (0)113 343 1723.
Disabled applicants are not obliged to inform employers of their disability but will still be covered by
the Equality Act once their disability becomes known.
Further information for applicants with disabilities, impairments or health conditions is
available in the applicant guidance.