Supplementary Material
Molecular Imaging and Biology
Comparison of PET imaging with 64Cu-liposomes and 18F-FDG in the 7,12–
dimethylbenz[a]anthracene (DMBA) induced hamster buccal pouch model of oral
dysplasia and squamous cell carcinoma
Lisa M. Mahakian1, D. Gregory Farwell2, Hua Zhang1 , Jai Woong Seo1, Brian Poirier3, Steven P.
Tinling4, Alaa M Afify3, Eric M. Haynam1, David Shaye 2, and Katherine W. Ferrara1*
1
Department of Biomedical Engineering, University of California, Davis. 451 Health Sciences
Drive, Davis, CA 95616
2
Department of Otolaryngology-Head & Neck Surgery, University of California, Davis. 2521
Stockton Blvd suite 7200, Sacramento, CA 95817
3
Department of Medical Pathology & Laboratory Medicine, University of California, Davis.
4400 V Street, Sacramento, CA 95817
4
Department of Otolaryngology-Research Laboratories, University of California, Davis. 1515
Newton Court room 211, Davis, CA 95618
*Corresponding author, e-mail address: kwferrara@ucdavis.edu.
a
b
18F-FDG
Supplementary Figure 1: Tracers employed in this study. a. Long circulating 100nm liposomes
HSPC:Cholesterol:DSPE-PEG2K-Ome:(6-BAT lipid) = (55:39:5:0.3, mol/mol), onto which 64Cu
was chelated. These particles circulate and accumulate within tumors. b. 18F-FDG is injected 30
minutes prior to PET scan and radioactivity accumulates in areas with active glucose metabolism.
b
a
d
c
e
Supplementary Figure 2: Optical images of mucosa. a, an example of normal, healthy mucosa
from an untreated hamster, with an arrow pointing to healthy tissue; b and c, examples of a very
early mucosal change: mucosa is thickened and red with white patches, the arrow in b pointing to
a white patch, the arrow in c pointing to a very small (<1mm) lesion; d and e, examples of later
lesions: mucosa is very thick, dark red and has an overall bumpy appearance, the arrows pointing
to large lesions (>2mm).