1 Chapter 14 Problem Set: Preparing Laboratory Solutions That Contain More Than One Solute 1. This question concerns the following multi-component solution: Solution A 1 M NaOH (FW = 40) 10% SDS A. Suppose that you were going to make 500 mL of Solution A from pure NaOH and SDS. Determine how much of each component you will need: Amount NaOH required: _____________ Amount SDS required: _____________ B. Suppose that you are going to make 250 mL of Solution A using a 10 M NaOH stock solution and a 20% SDS stock solution. How much of each stock solution will you need to use? Volume 10 M NaOH required: _____________ Volume 20% SDS required: _____________ 2. This question concerns the following multi-component solution: 2 TES 100 mM Tris 10 mM EDTA 1% SDS You are going to make up 350 mL of TES from the following stock solutions: 1.5 M Tris, 0.5 M EDTA, and 20% SDS. Write a protocol for how you will prepare this solution: 3. This question concerns the following multi-component solution: 10X ligation buffer 600 mM Tris, pH 7.6 100 mM MgCl2 70 mM DTT If you take 150 uL of 10X ligation buffer and add 1350 uL of water, what is the concentration of each of the components following dilution? Concentration of Tris = ______________ Concentration of MgCl2 = ______________ Concentration of DTT = ______________ 4. This question concerns the following multi-component solution recipe: 3 To make 1 L of Solution X: Add: 5 mL 10 mL 25 mL 1 M MgCl2 0.4% thymidine 20% glucose Mix, and add enough water to bring the final volume to 1 L. What are the final concentrations of each component in Solution X? Concentration of MgCl2 = ______________ Concentration of thymidine = ______________ Concentration of glucose = ______________

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# Homework #1: Scientific Notation and Exponents (Chapter - Bio-Link