Dr. Kerry Bloom & Dr. Blaire Steinwand
Fall 2014
TR, 12:30-01:45
GS200
Section 007
Lecture Schedule: Part I (Bloom)
Date
#
Pre-lecture
assigned readings
Objectives
1
IntroductionGenes,
Genomes and
Genetic
Analysis
Chap. 1
Explain how genetics effects our lives
Tu
8/19
Understand the terms:
Personalized medicine, Genetically
modified organisms, stem cells
Kerry Bloom
2
Th
DNA: the
Molecule of
Heredity
Chap 7 223-231
Define Gene, Chromosome, allele
Define genotype, phenotype
Explain why DNA is the heredity material
Draw the chemical structure of DNA and
define the specific features responsible for
base pairing, strand complementarity,
antiparallel helix
How traits are
transmitted:
Mendelian
Inheritance
Chap 2 25-44
Define the features of Mendels
experiments
Explain the Law of Segregation
Explain the Law of Independent
Assortment
Explain what a di-hybrid cross is and
what it tells us.
8/21
3
Tu
8/26
Recommended
readings
Assignments
Understand Dominance and Recessive
4
Mitosis
Chap 3 63-81
Mitosis
Th 8/28
5
Tu
The
chromosome
theory of
Inheritance
Chap 3 81-96
9/02
6
Understand the Unit of Inheritance
Explain how sex chromosomes help us
understand heredity processes
Explain the process of chromosome nondisjunction
Gene
Interaction
Chap 4 105-142
Th
Define incomplete penetrance and
variable expressivity
Define epistatis and pleiotropy
9/04
For lectures 7,8 work linkage mapping
7
Genetic
Chap 5 143Linkage and
164
Tu
Chromosome
Mapping
9/09
Define gene linkage and map distance
Explain how recombination frequency is
determined
8
.
Tetrads
Chap 5 170177
Th 9/11
Monday Review Session Coker 201 6:30 to 8:00
Tu
9/16
EXAM 1 (Lectures 1-8)
9
Th
Molecular
Mechanisms of
Mutation and
DNA Repair
Chap. 12 383408
Define mutation and why they are
heritable.
Explain the difference between induced
and spontaneous mutations.
Define DNA repair
Molecular
Biology of
DNA
Recombination
and Exchange
Chap 12 410421
Define the Holliday model for DNA
recombination.
Chromosome
Structure
Chap 11 359382
Explain the difference between DNA and
the chromosome.
Define Chromatin.
Define Epigenetics
Chromosome
Aberrations
Chap 13 422443
How does non-disjunction lead to changes
in chromosome number.
Explain the mechanisms that lead to
chromosome rearrangement
9/18
10
Tu
9/23
11
Explain what is meant by Gene
Conversion
Th
9/25
12
Tu
09/30
13
Th
Chromosome
Dynamics and
Fractals
10/02
Recitation: 10/2-3 Exam review questions
Tu
10/07
EXAM 2 (Lectures 8-13)
What is meant by DNA fluctuation.
Define 3C and how this was used to map
higher order chromosome conformation
14
Th
How Genes
TravelBacterial
Genetics
-Describe the different mechanisms of horizontal
gene transfer
Blaire
Steinwand
- Build hypotheses, design an experiment using an
appropriate assay, and predict the results
pp. 184-191
- Explain how one can examine the phenotypes of
bacteria
10/09
- Describe bacterial conjugation
No Recitations
15
Bacterial
Genetics II +
Tu
introduction to
Gene
10/14
Expression
pp. 261-264
(section 8.1)
- Compare and contrast F-, F+, and Hfr bacterial
strains
- Explain how the interrupted mating technique
allows us to determine gene order
- Describe the central dogma
- Describe the differences between DNA and RNA
- Predict the results of an experiment based on a
given hypothesis
Th
10/16
16
Tu
- pp. 191-194
- “mRNA
Brenner
experiment
explained.pdf” (External
Links/Sakai)
Gene
ExpressionTranscription
pp. 264-265
- Explain how the RNA polymerase knows where
to start transcription
- Homework due Wed,
10/23
- Compare and contrast prokaryotic and eukaryotic
transcription mechanisms
pp. 270-271; 273- - MG- Promoter quiz, due
274;
Tue, 10/22, 10:00am
Fig. 8.6
- Homework due Wed, 10/30
-. Describe the event staking place during
transcription
Th
MG (MasteringGenetics)
RNA quiz, due Tue, 10/15
10:00am
Fall Break
10/21
17
-
Gene
ExpressionTranslation
10/23
- Explain how pre-mRNA is processed
- Describe the mechanisms of RNA splicing and
define alternative splicing
- Define and identify an open reading frame
- Explain the forces that shape a protein and
determine its function
- Define the genetic code
Recitation: 10/23-24 Bacterial Genetics activity
pp. 278-284
18
Tu
10/28
19
Th
Gene
ExpressionThe Nature of
the Genetic
Code
Gene
Regulation in
Prokaryotes
Figures 9.7;
9.9; and 9.10
(and the
relevant text)
-
199 (including
Figure 6.12)
- Describe the events that take place during
translation
- Define the properties of the genetic code
pp. 312-322
(sections 9.4 and
9.5)
- MG- Translation quiz, due
Tue, 10/29, 10:00am
Homework due Wed, 11/06
- Explain how the code has been deciphered
- Predict the effect of different mutations on
protein function
- List the various ways that gene expression can be
regulated
-PeerWise questions (Gene
expression), due Tue, 11/5
pp. 463-471; 473
- Describe what the lac operon does and why E.
coli regulates its expression
-PeerWise answers and
feedback (Gene
expression), due Sun, 11/10
10/30
-Describe how lactose induces the expression of the
lac operon
- Deduce the genes affected in the lac operon by
their phenotypes and genetic behavior
Recitation: 10/30-31 Gene Expression activity
20
Tu
Gene
Regulation in
Prokaryotes II
-
Homework due Wed, 11/13
11/04
21
Th
Gene
Regulation in
Eukaryotes
- Describe cis- and trans- active elements involved
in eukaryotic regulation
pp. 501-504; 509512
- Define epigenetics and explain how epigenetic
mechanisms regulate transcription
11/06
- Explain imprinting and chromatin remodeling
Recitation: 11/6-7 Regulation of Gene Expression in prokaryotes
Tu
EXAM 3 (Lectures 13-20)
11/11
22
Gene
Regulation in
- Discuss the effect of the environment on the
epigenome
pp. 283; 285-286;
512-516
-PeerWise questions (Gene
expression), due Tue, 11/26
Th
- Explain the role of alternative splicing in gene
regulation, and how it is regulated
Eukaryotes II
11/13
-PeerWise answers and
feedback (Gene
expression), due Tue, 12/03
- Describe the role of miRNA in gene regulation
-
- Homework due Wed, 11/20
Recitation: 11/13-14 Regulation of Gene Expression in Eukaryotes
23
Tu
Recombinant
DNA
Technology
pp. 531-534
11/18
24
Th
11/20
- Distinguish between forward and reverse genetics
- Define developmental genetics and explain the
importance of model organisms
Recombinant
DNA
Technology: A
PHD in 75
minutes
- Design a protocol for gene cloning
- Distinguish between genomic and complimentary
DNA
- Describe the main properties of cancer cells
- Define the three groups of cancer promoting
mutations
11/25
Thanksgiving Break
- Define the three groups of cancer promoting
mutations
Tu 12/02
Molecular
Genetics of
Cancer II
Tu 12/10
EXAM 4 & CUMULATIVE FINAL EXAM
12:00 pm
- Describe the genomic approaches in cancer
therapy
- MG- Restriction quiz, due
Tue, 11/19, 10:00am
-
- Explain how to construct molecules to address
biological questions
Recitation: 11/20-21 Exam review questions
25
Molecular
Genetics of
Tu
Cancer
Th
11/27
26
-
pp. 535-539
- Homework due Wed, 11/27