Department of Physiology
Procedure No:
PHY/SOP/ER01
Revision No:
002
Title:
Effective Date :
15/8/2011
Biological Spill Response
(Adopted from OSHE/SOP/BS/05)
Page:
Page 1 of 3
Safety & Health
Laboratory (Location):
Cytokine biology Lab, MD9
Prepared/Review By
Approved By
Next Review Date
Oh Chu Yun
Dr. Leung Pui Lam Bernard
14/8/2014
* Review Date = Future date for the next revision (every 3yrs)
1.0 OBJECTIVE
The objective of this document is to provide guidance on how biological incidents are to be
handled and contained safely to minimize the risk of contamination. It also gives details to the
laboratory personnel on how to protect them and how to respond to spills. This SOP is
applicable for all personnel working in Cytokine Biology Laboratory.
2.0 RESPONSIBILITIES
2.1 Principal Investigator
The PI is responsible for:
a. Assessing the incident and the course of action required to control the incident
effectively.
b. Ensuring that an incident report is submitted to the relevant parties such as Head of
Department, Deanry or OSHE, via AIRS.
c. Implementing any corrective measures as stipulated by the Head of Department, Deanry
or OSHE.
3.0 PROCEDURES
3.1 Response
3.1.1 Spills inside a Biological Safety Cabinet
a. Leave the cabinet turned on.
b. Put on gloves and a lab coat.
c. Spray or wipe cabinet walls, work surfaces, and equipment with disinfectant
equivalent to a 1:10 bleach solution. If necessary, flood the work surface
including drain pans and catch basins below the work surface with disinfectant.
d. Wait at least 20 minutes.
e. Soak up disinfectant and spill with paper towels. Drain catch basin into a
container. Lift front exhaust grill and tray and wipe all surfaces. Ensure that no
paper towels or solid debris are blown into the area beneath the grill.
Department of Physiology
Procedure No:
PHY/SOP/ER01
Revision No:
002
Title:
Effective Date :
15/8/2011
Biological Spill Response
(Adopted from OSHE/SOP/BS/05)
Page:
Page 2 of 3
Safety & Health
Laboratory (Location):
f.
g.
Cytokine biology Lab, MD9
Double-bag packed all clean-up materials in biohazard bag, disposal via
contractor.
Wash hands with appropriate soap/disinfectant and any exposed surfaces
thoroughly after the clean-up procedure.
3.1.2 Spill outside a Biological Safety Cabinet in a BSL 1 Lab
This is for spills that can be covered by a few paper towels.
a. Notify others in the area, to prevent contamination of additional personnel and
environment
b. Put on gloves and a lab coat.
c. Cover spill with paper towels and gently apply disinfectant, proceeding from the
outer edge of the spill to its center.
d. Leave in place for at least 30 minutes
e. Pick up the towels and discard into a biohazard container. Use forceps to pick
up any broken glass and place them into a sharps container.
f. Re-wipe the spill area with disinfectant.
g. Remove gloves and thoroughly wash hands.
3.1.3 Biological spill outside a Biological Safety Cabinet in BSL2 Lab
a. Hold your breath and leave the room immediately.
b. Warn others to stay out of the spill area to prevent spread of contamination.
c. Post a sign on the door warning others of the spill.
d. Remove any contaminated clothing and put it into a biohazard bag for
autoclaving.
e. Wash hands and exposed skin and inform your PI or supervisor about the spill.
f. Put on protective clothing (lab coat, gloves, mask, eye protection, shoe covers)
and assemble clean-up materials.
g. Wait 30 minutes before re-entering the contaminated area to allow
dissipation/settling of aerosols.
h. Cover the spill with paper towels and gently apply disinfectant, proceeding from
the outer edge of the spill to its center. Refer to MSDS for appropriate type of
disinfectant.
i. Leave in place for at least 30 minutes. (Refer to MSDS for contact time).
j. Collect all treated materials and discard in a biohazard container. Use forceps
to pick up any broken glass and place in a sharps container.
k. Re-wipe the spill area with disinfectant.
l. Remove gloves and wash hands thoroughly.
m. Dispose biohazard wastes according to procedures.
3.2 WASTE DISPOSAL
Department of Physiology
Procedure No:
PHY/SOP/ER01
Revision No:
002
Title:
Effective Date :
15/8/2011
Biological Spill Response
(Adopted from OSHE/SOP/BS/05)
Page:
Page 3 of 3
Safety & Health
Laboratory (Location):
Cytokine biology Lab, MD9
Refer to SOP on waste disposal of biohazard wastes (CBL/SOP/04)
4.0 RECORDS
The incident report must be completed and submitted (refer to SOP on incident reporting OSHE/SOP/U/02).
5.0 REFERENCES
a. SOP on Biological Waste Disposal (CBL/SOP/04)
b. SOP on incident reporting (OSHE/SOP/U/02)
6.0 APPENDICES
Nil