Peptide/Protein Analysis Request
Customer Supplied Sample Submission Form
User Information
Name:
NEP Quote/Order # :
Today’s Date:
AAA SERVICES:
Amino Acid Analysis (choose one below)
Single Analysis ($150 per sample)
Duplicate Analysis ($300 per sample)
Triplicate Analysis ($450 per sample)
Select Reporting Information
Concentration or Total Sample Amount, *please see below for amount and concentration needed..
*Concentration measurement: Requires a minimum of 20 µL per analysis (measured volumetrically) of a 0.1 mg/mL solution
of peptide or protein. Liquid samples should be submitted in a sealed (parafilmed) 500 µL centrifuge tube on dry ice. (Accuracy
of measurement depends on the sample not evaporating and the volume being measured accurately). If you want NEP to
measure the volume (that is, you do not have calibrated pipettes), please send at least 30 µL of 0.1 mg/mL solution. If you do
not have 500 µL centrifuge tubes (e.g. VWR cat. no. 89000-010), NEP can provide you with these.
Net Peptide Content, ** please see below for amount needed.
**Net Peptide Content: Requires a minimum of 5.0 mg per analysis of dry material.
ANALYTICAL SERVICES:
LC-MS; Provides purity and identity ($195 per sample)
HPLC; Provides purity ($150 per sample)
Mass Spec; Provides identity ($150 per sample)
For all of the analytical options above, please provide 0.5 to 1.0mg per sample
Email address to receive data (Data is sent electronically only, unless requested otherwise.)
Sample Information
Please input sample information in table on next page.
Submission
Mail samples with hardcopy of this completed form to:
Attn: Michelle Lewis
New England Peptide
65 Zub Lane
Gardner, MA 01440
Ref: NEP Quote/Order Number
Also email this form to [email protected] at time of submission.
Please be sure to note NEP’s
Quote/Order number on
package or as part of the
address
Peptide/Protein Analysis Request
Customer Supplied Sample Submission Form
Sample Name
Example 1
Sequence or Ratio of Amino Acids
H2N-EXAMPLE-OH
*Please be sure to paste sequences/ratios in TEXT FORMAT
Molecular
Weight
988
Concentration
1.56mg/mL
Total
Amount
Sent
0.5mL
Buffer
Components
Number
of
Analyses
Customer
Comments/
Notes for NEP
PBS
2
Please provide
results in XX
units
Peptide/Protein Analysis Request
Customer Supplied Sample Submission Form
Sample/Buffer Components that Interfere with Amino Acid Analysis
Any buffers or additives that include primary or secondary amine groups can interfere with hydrolysis or skew
quantitative results in AAA. Such components should be avoided if possible. For example, glycine buffers and Tris
buffers should not be used for samples being submitted for AAA.
Additionally, alcohol-containing buffer components such as glycerol or mannitol cause degradation of certain
amino acids.
Proteins in PAGE gels or that have acrylamide contaminants cannot be analyzed. Proteins adsorbed onto
nitrocellulose filters also cannot be analyzed.
Analysis cannot be performed with samples containing detergents or antibiotics.
High salt or highly acidic or basic buffers can cause instrument failure and/or interfere with hydrolysis. Proteins or
peptides in PBS or PBS-like buffers (50mM phosphate, 150mM Salt, pH ~7) buffers analyze well, but higher
amounts of salt or extremes of pH should be avoided.
Samples that contain any of these contaminants can be analyzed, but will require a buffer exchange before AAA
can occur. There will be an additional charge for such services.
**Please contact us for more information on acceptable buffer components. Direct any questions
[email protected]
Download

Peptide/Protein Analysis Request Customer Supplied Sample