記錄
編號
12137
狀態
G0498856037
助教
查核
建檔完成
索書
號
查核完成
學校
名稱
輔仁大學
系所
名稱
食品科學系
舊系
所名
稱
學號
498856037
研究
吳冠彥
生(中)
研究
生(英)
論文
名稱
(中)
啤酒渣機能性成分分析暨其抑制肥胖之活性
論文
名稱
(英)
Analysis of functional components in beer lee and its antiobesity activities
其他
題名
指導
教授
(中)
高彩華
指導
教授
(英)
校內
全文
開放
日期
2017.3.30
校外
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2017.3.30
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01
學位
類別
碩士
畢業
學年
度
100
出版
年
101
語文
別
中文
關鍵
啤酒渣 異戊烯類黃酮 啤酒花苦味酸 肥胖 3T3-L1
字(中)
關鍵
字(英)
摘要
(中)
啤酒是全球最普遍的酒精飲料之一並富含多種營養成分與機能性成分。
啤酒渣泛指啤酒釀造過程所產生的麥粕、啤酒花粕與酵母等副產物,一
般作為飼料或肥料。由於啤酒渣中機能性成分相關研究較為缺乏,因此
本研究目的即是先以高效液相層析儀分析啤酒渣中異戊烯類黃酮與啤酒
花苦味酸之種類與含量,並以 3T3-L1 細胞株探討啤酒渣萃取物對細胞
增生、細胞凋亡及脂肪生成之影響。結果顯示,使用 Thermo HyPURITY
C18 管柱(150 mm × 4.6 mm I.D., 5 μm)搭配光電二極體陣列偵檢器,梯度
動相系統分別為磷酸水溶液(pH 1.6)及氰甲烷,以流速 1.5 mL/min 進行沖
提,檢測波長為 314 nm,可在 29 分鐘內同步分離 isoxanthohumol、
xanthohumol、8-prenylnaringenin 及 6-prenylnaringenin 4 種異戊烯類黃酮與
cohumulone、humulone、adhumulone、isocohumulone、isohumulone、
isoadhumulone、colupulone、lupulone 及 adlupulone 9 種啤酒花苦味酸。而
啤酒渣中則含有 isoxanthohumol、xanthohumol、8-prenylnaringenin、6prenylnaringenin、cohumulone、humulone、adhumulone、colupulone、
lupulone 及 adlupulone。在細胞增生方面,前驅脂肪細胞與脂肪細胞之存
活率隨著啤酒渣萃取物濃度的提升而下降,IC50 分別是 7.3 μg/mL 與
10.4 μg/mL。由流式細胞儀 annexin V-FITC/PI 雙染結果指出,啤酒渣萃
取物會促使成熟脂肪細胞凋亡,但並未造成前驅脂肪細胞凋亡。細胞週
期分析結果顯示,經啤酒渣萃取物處理後會使前驅脂肪細胞停滯於
G2/M 期,且細胞週期相關蛋白質 cyclin B1 顯著提升。在抑制脂肪生成
方面,在前驅脂肪細胞分化成脂肪細胞過程中添加啤酒渣萃取物,能有
效降低脂肪細胞數目及減少三酸甘油酯形成。
摘要
(英)
論文
目次
Beer is one of the main alcoholic beverages in the world, which is rich in nutrition
and contains various functional compounds. Beer lee is a by-product generated
from beer brewing and composed with spent grains, spent hops and surplus yeast,
which have been used as cattle feed or fertilizer. However, studies of the amount
of functional compounds in beer lee are rare. The objectives of this research are to
analyze the content and variety of prenylflavonoid and hop bitter acid in beer lee
by using high performance liquid chromatography, and to discuss the
antiproliferation, apoptosis, and antiadipogenesis activity of 3T3-L1 cell line as
affected by ethanol extract from beer lee. Results showed that 4 of
prenylflavonoid and 9 of hop bitter acid can be separated simultaneously in 29
minutes by employing a Thermo HyPURITY C18 column (150 mm × 4.6 mm
I.D., 5 μm) with the gradient solvent system of phosphoric acid in water (pH 1.6)
and acetonitrile at flow rate 1.5 mL/min and detection wavelength at 314 nm.
Moreover, the variety of these compounds can be identified as isoxanthohumol,
xanthohumol, 8-prenylnaringenin, 6-prenylnaringenin, cohumulone, humulone,
adhumulone, colupulone, lupulone and adlupulone in beer lee. In cell proliferation
test, the preadipocyte and adipocyte viability decreased by increasing the
concentration of beer lee extract and showed a 50% inhibition at 7.3 μg/mL and
10.4 μg/mL. Apoptosis analysis showed that beer lee extract increased the
number of apoptotic cells in adipocyte, but not in preadipocyte by FITC-Annexin
V/propidium iodine apoptosis flow cytometry. Cell cycle analysis revealed that
treatment of preadipocyte with beer lee extract caused cell cycle arrest at the
G2/M phase, and the cyclin B1 expression also shown to be up-regulated. During
differentiation of 3T3-L1 preadipocytes to adipocytes, the beer lee extract
efficiently reduced adipocyte number and the generation of intracellular
triglyceride by oil red staining and triglyceride assay respectively.
第一章、緒言 1 第二章、文獻回顧 2 一、 啤酒渣簡介 2 (一) 麥粕 2 (二)
啤酒花粕 5 (三) 酵母 5 二、 啤酒渣中機能性成分 5 (一) 異戊烯類黃酮 8
(二) 啤酒花苦味酸 18 三、 肥胖簡介 33 (一) 成因 35 (二) 脂肪組織 35
(三) 3T3-L1 脂肪細胞株 35 第三章、材料與方法 39 一、 研究架構 39
二、 材料與儀器 40 (一) 原料 40 (二) 化學藥品 40 (三) 儀器設備 43 三、
實驗方法 45 (一) 啤酒渣之基本成分分析 45 (二) 啤酒渣異戊烯類黃酮及
啤酒花苦味酸成分分析 48 (三) 以細胞模式探討啤酒渣萃取液抑制肥胖之
活性 53 (四) 統計分析 64 第四章、結果與討論 65 一、 啤酒渣之組成 65
二、 HPLC 同步分析異戊烯類黃酮與啤酒花苦味酸方法之評估 65 (一) 偵
測波長之評估 65 (二) 管柱之評估 69 (三) 動相系統的發展 71 三、 波峰
成分鑑定 80 (一) 啤酒花苦味酸標準品波峰鑑定 80 (二) 樣品波峰鑑定 80
(三) 分析確效性評估 90 (四) 啤酒渣中異戊烯類黃酮與啤酒花苦味酸之含
量 93 四、 體外細胞模式探討啤酒渣萃取液抑制肥胖之效果 96 (一) 抑制
3T3-L1 細胞存活之影響 96 (二) 啤酒渣萃取物對凋亡之影響 100 (三) 啤酒
渣萃取物對細胞週期之影響 104 (四) 啤酒渣萃取物對脂肪生成之影響
107 第五章、結論 112 第六章、參考文獻 113
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