1
Supplementary material
2
List of media used
3
Medium formula
4
SYP agar medium with artificial sea water
5
Soluble starch
5
g
6
Yeast extract
2
g
7
Peptone
1
g
8
Artificial sea salt
15
g
9
Agar
7.5 g
10
Distilled water to
500 ml
11
The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
12
C for 20 minutes
13
SYP broth medium with artificial sea water
14
Soluble starch
5
g
15
Yeast extract
2
g
16
Peptone
1
g
17
Artificial sea salt
15
g
18
Distilled water to
500 ml
19
The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
20
C for 20 minutes
21
22
23
24
25
1
SYP broth medium
2
Soluble starch
5
g
3
Yeast extract
2
g
4
Peptone
1
g
5
Distilled water to
500 ml
6
Dissolve all components and autoclave at 121°C for 20 min. pH was not adjusted.
7
8
SYP broth medium supplemented with 0.01%NaCl (SYP-0.01%NaCl)
9
Soluble starch
5
g
10
Yeast extract
2
g
11
Peptone
1
g
12
NaCl
0.0015 g
13
Distilled water to
500 ml
14
The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
15
C for 20 minutes
16
17
SYP broth medium supplemented with 1%NaCl (SYP-1%NaCl)
18
Soluble starch
5
g
19
Yeast extract
2
g
20
Peptone
1
g
21
NaCl
5
g
22
Distilled water to
500 ml
23
The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
24
C for 20 minutes
25
1
SYP broth medium supplemented with 3%NaCl (SYP-3%NaCl)
2
Soluble starch
5
g
3
Yeast extract
2
g
4
Peptone
1
g
5
NaCl
15
g
6
Distilled water to
500 ml
7
The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
8
C for 20 minutes
9
SYP broth medium supplemented with 6%NaCl (SYP-6%NaCl)
10
Soluble starch
5
g
11
Yeast extract
2
g
12
Peptone
1
g
13
NaCl
30
g
14
Distilled water to
500 ml
15
The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
16
C for 20 minutes
17
SYP broth medium supplemented with 1%KCl (SYP-1%KCl)
18
Soluble starch
5
g
19
Yeast extract
2
g
20
Peptone
1
g
21
KCl
15
g
22
Distilled water to
500 ml
23
The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
24
C for 20 minutes
25
1
SYP broth medium supplemented with 3%KCl (SYP-3%KCl)
2
Soluble starch
5
g
3
Yeast extract
2
g
4
Peptone
1
g
5
KCl
15
g
6
Distilled water to
500 ml
7
The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
8
C
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
1
2
3
Figure: Identification of rifamycin B (A,B) Q-TOF-MS and UV-visible detected chromatogram
4
of S.arenicola broth extracts indicated by a peak at 12 min suggest the presence of rifamycin
5
class compound.(C) Mass spectrum of S. arenicola M413 extract at 12 min, indicating the
6
presence of rifamycin B (754.3105 m/z). (D) UV spectrum (430nm) of S.arenicola M413
7
extract at 12 min. (E) UV spectrum (430) of rifamycin B standard at 12 min.
1
Binning data:
2
Comparisons of resolutions of binned data
A: 9000 variables – unbinned
B: 4500 variables – bin size 2
C: 1800 variables – bin size 5
D: 900 variables – bin size 10
E: 450 variables – bin size 20
F: 180 variables – bin size 50
3
4
There is very little difference in peak resolution between unbinned , bin 2, bin 5, bin 10 but
5
larger bins (20, 50) cause resolution loss and a marked increase in baseline. The benefit of
6
binning is to minimise any errors due to retention time differences and to reduce the data
7
complexity/computational load. The complete dataset is reduced from 63000 data points (9000
8
time slices x 7 wavelengths) to 6300 with little or no loss of data/information
9
10
Download

jam12507-sup-0001-DataS1-FigureS1