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Supplementary material
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List of media used
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Medium formula
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SYP agar medium with artificial sea water
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Soluble starch
5
g
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Yeast extract
2
g
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Peptone
1
g
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Artificial sea salt
15
g
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Agar
7.5 g
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Distilled water to
500 ml
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The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
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C for 20 minutes
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SYP broth medium with artificial sea water
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Soluble starch
5
g
15
Yeast extract
2
g
16
Peptone
1
g
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Artificial sea salt
15
g
18
Distilled water to
500 ml
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The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
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C for 20 minutes
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SYP broth medium
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Soluble starch
5
g
3
Yeast extract
2
g
4
Peptone
1
g
5
Distilled water to
500 ml
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Dissolve all components and autoclave at 121°C for 20 min. pH was not adjusted.
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SYP broth medium supplemented with 0.01%NaCl (SYP-0.01%NaCl)
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Soluble starch
5
g
10
Yeast extract
2
g
11
Peptone
1
g
12
NaCl
0.0015 g
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Distilled water to
500 ml
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The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
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C for 20 minutes
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SYP broth medium supplemented with 1%NaCl (SYP-1%NaCl)
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Soluble starch
5
g
19
Yeast extract
2
g
20
Peptone
1
g
21
NaCl
5
g
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Distilled water to
500 ml
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The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
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C for 20 minutes
25
1
SYP broth medium supplemented with 3%NaCl (SYP-3%NaCl)
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Soluble starch
5
g
3
Yeast extract
2
g
4
Peptone
1
g
5
NaCl
15
g
6
Distilled water to
500 ml
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The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
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C for 20 minutes
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SYP broth medium supplemented with 6%NaCl (SYP-6%NaCl)
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Soluble starch
5
g
11
Yeast extract
2
g
12
Peptone
1
g
13
NaCl
30
g
14
Distilled water to
500 ml
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The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
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C for 20 minutes
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SYP broth medium supplemented with 1%KCl (SYP-1%KCl)
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Soluble starch
5
g
19
Yeast extract
2
g
20
Peptone
1
g
21
KCl
15
g
22
Distilled water to
500 ml
23
The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
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C for 20 minutes
25
1
SYP broth medium supplemented with 3%KCl (SYP-3%KCl)
2
Soluble starch
5
g
3
Yeast extract
2
g
4
Peptone
1
g
5
KCl
15
g
6
Distilled water to
500 ml
7
The pH was adjusted to between 8.0 and 8.3 with sodium hydroxide before autoclaving at 121 ͦ
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C
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12
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Figure: Identification of rifamycin B (A,B) Q-TOF-MS and UV-visible detected chromatogram
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of S.arenicola broth extracts indicated by a peak at 12 min suggest the presence of rifamycin
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class compound.(C) Mass spectrum of S. arenicola M413 extract at 12 min, indicating the
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presence of rifamycin B (754.3105 m/z). (D) UV spectrum (430nm) of S.arenicola M413
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extract at 12 min. (E) UV spectrum (430) of rifamycin B standard at 12 min.
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Binning data:
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Comparisons of resolutions of binned data
A: 9000 variables – unbinned
B: 4500 variables – bin size 2
C: 1800 variables – bin size 5
D: 900 variables – bin size 10
E: 450 variables – bin size 20
F: 180 variables – bin size 50
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There is very little difference in peak resolution between unbinned , bin 2, bin 5, bin 10 but
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larger bins (20, 50) cause resolution loss and a marked increase in baseline. The benefit of
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binning is to minimise any errors due to retention time differences and to reduce the data
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complexity/computational load. The complete dataset is reduced from 63000 data points (9000
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time slices x 7 wavelengths) to 6300 with little or no loss of data/information
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