DNA Fingerprinting For some background information useful in your DNA Fingerprinting Performance, Visit this website: http://www.teachersdomain.org/asset/tdc02_int_creatednafp2/ and complete the guiding questions below. The process of gel electrophoresis can be used to create a DNA fingerprint. Name two situations in which DNA fingerprints can be used. 1. 2. Step 1: Add restriction enzymes to the DNA sample. What do restriction enzymes do? Step 2: Pour the agarose gel into the tray. The gel is porous. How does it help separate the DNA fragments? Step 3: Pour the DNA into the tray. Step 4: Turn on the gel electrophoresis. Define Electrophoresis: DNA has which charge? Which charge is the DNA attracted to? Step 5: Place the nylon membrane on the gel. What does this do? Step 6: Add the probes. How do the probes know which piece of DNA to bind to? What special property do the probes have? Step 7: Add the X-ray film. Why? Step 8: Develop the film. What does this create? The short version of how DNA fingerprints are made: DNA has a ( ) charged. When you turn on the gel, the DNA samples move across to the ( ) side. Because the gel is porous, ______ fragments get stuck and don’t move far whereas _______ fragments pass more easily and move farther. Whose blood is it? Who are the soldier’s parents & why? Questions adapted from Judy Jones @ http://www.jonesbiology.com/biology-1/chapter-123---genetic-engineering After you use the guiding questions to familiarize yourself with DNA Fingerprint technology, you need to complete a performance explaining the process. In a group of 3, you may complete a: Song Rap Skit Cartoon The length/written requirements are: Song or Rap must be no less than 5 stanzas and group must turn in one neat handwritten copy of the lyrics Skit must have no less than 5 thorough spoken “lines” and group must turn in one neat handwritten copy of the script. Cartoon must have no less than 5 distinct “panels” with detailed captions incorporating the key phrases The [thorough explanation/accurate use of] key phrases required are: DNA Fingerprinting Gel Electrophoresis Restriction Enzymes Agarose Gel Specific base sequence DNA Sample Positive Charge Negative Charge Paternity Criminal Identification Larger fragments Smaller fragment For greater clarification on what is expected, see the rubric; Happy writing and/or drawing!