DECLARATION OF EMERGENT PHENOTYPE
AND REQUEST FOR A PROCEDURAL CHANGE TO A MURINE PROTOCOL
Submitted to the University of South Florida, Institutional Animal Care & Use Committee,
MDC 35, phone 974-7106, fax 974-7091, email IACUC@usf.edu
PHS #A4100-01; USDA #58-R-0015; AAALAC #000434
1. TITLE OF PROJECT.
2. INVESTIGATOR.
Principal Investigator
Department
Certification Number
Phone
3. IACUC PROTOCOL.
The Principal Investigator proposes to amend IACUC file(s) #
the following observations and so that the indicated procedural change(s) can be implemented.
in order to declare
4. IDENTIFICATION.
This request pertains to the following previously approved strain/line/genotype.
5. EMERGENT PHENOTYPE.
In the course of breeding production and characterization of the strain/line/genotype indicated above, the unanticipated features of the
emergent phenotype, or the recurring health problem or condition have been observed to develop, as described below.
6. MONITORING, TREATMENT & CLINICAL ENDPOINT.
The clinical monitoring and treatment(s) that will be used to detect and alleviate any potential for discomfort associated with the
observed phenotype feature(s) or recurring health problem or condition are described below. The clinical endpoint that results in
humane euthanasia of an affected animal is also indicated below.
Note: The earliest possible clinical endpoint must be used (refer to IACUC Principle & Procedure XII.16). If no treatment will be implemented, justify
why treatments are not required or will interfere with the production and characterization of the animals.
RIC IACUC DC #0010.2
Effective 9/14
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7. CATEGORY OF RESEARCH.
Are features of the phenotype, or the recurring health problem or condition expected to produce pain or discomfort that cannot or will
not be alleviated by the administration of appropriate anesthetics, analgesics, or treatments?
No:
. This request is complete. Yes:
. Within the space below, justify why the originally described Category of Research
must be altered to Category C, indicate the number of animals and the proportion of the colony so affected, re-emphasize to the IACUC
the expected utility of the colony, and indicate why clinical intervention conflicts with the purposes of the proposed colony.
8. CERTIFICATION.
The PI certifies that this request to move previously approved animals to Category C has been submitted only after a search for
alternatives and alternative methods, including a search of the following relevant database(s) indicated below, covering the indicated
years (at least the last 10 yrs.), and using the indicated search term(s), has demonstrated that suitable alternatives to these procedures,
and to aspects of these procedures which may cause pain or discomfort to animals, or to this animal use are not available or applicable.
Database:
Years:
_____________________________________________
Signature of Principal Investigator
Search Terms:
________________________________
Date
These requests are reviewed by the IACUC via the Designated Member Review Process. Written IACUC approval is required of these proposed
changes prior to their implementation.
RIC IACUC DC #0010.2
Effective 9/14
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