OIE Reference Laboratory Reports
Activities in 2012
Name of disease (or topic) for which you
are a designated OIE Reference
Laboratory:
Brucellosis
Address of laboratory
Agence nationale de sécurité sanitaire de
l’alimentation, de l’environnement et du
travail (ANSES)/
French Agency for Food, Environmental &
Occupational Health Safety
Laboratoire de Santé Animale de MaisonsAlfort / Maisons-Alfort Animal Health
Laboratory
Unité Zoonoses Bactériennes /
Bacterial Zoonoses Unit
23, avenue du Général-de-Gaulle
94706 Maisons-Alfort Cedex
France
Tel.:
+ 33 (0)1 49 77 13 00 (switch.) / 46 23 (secr.)
Fax:
+ 33 (0)1 49 77 13 44
e-mail address:
bruno.garin-bastuji@anses.fr
website:
www.anses.fr
Name (including Title) of Head of
Laboratory (Responsible Official):
Dr. Pascal BOIREAU
Name (including Title and Position) of OIE
Reference Expert:
Dr. Bruno GARIN-BASTUJI,
Director of the Animal Health Laboratory
DVM, Senior Research Director (DR1)
Head of the Bacterial Zoonoses Unit
Date of submission to the OIE
Annual reports of OIE Reference Centres, 2012
24 January 2013
1
OIE RL for « Brucellosis » – « B. Garin-Bastuji » – « France »
ToR: To use, promote and disseminate diagnostic methods validated according to OIE
Standards
Test
Total number of test performed last year
Indirect diagnostic tests
Nationally
Internationally
RBT
691
362 (122 Morocco, 240 EU)
SAT
221
122 (122 Morocco)
CFT
518
362 (122 Morocco, 240 EU)
I-ELISA (bovine serum)
145
122 (122 Morocco)
I-ELISA (bovine milk)
208
-
I-ELISA (porcine serum)
285
242 (240 EU, 2 Belgium)
C-ELISA
197
-
FPA
-
-
Milk Ring Test
181
-
B. ovis CFT
464
-
B. ovis I-ELISA
218
-
B. canis RSAT
-
-
B. canis LFIA
122
-
B. canis TAT
3
-
Direct diagnostic tests
Nationally
Internationally
Culture
356
-
Brucella identification and biotyping (animal str.)
109
3 (2 UK, 1 Argentina)
Brucella identification and biotyping (human str.)
24
-
Brucella PCR
530
24 Belgium
Brucella molecular typing
207
32 ( 2 UK, 1 Argentina, 29 Algeria)
Official control of diagnostic reagent batches
Nationally
Internationally
RBT, MRT, CFT, SAT antigens, ELISA & FPA kits
-
International reagent suppliers
10 RBT
5 CFT
2 SAT
8 I-ELISA Bovine Serum
2 I-ELISA Bovine Milk
Standard & control sera
-
15 secondary standard sera (EU NRLs)
2 positive control sera (commercial)
Brucellin
-
2
Official control of Brucella vaccines batches
Nationally
Internationally
Rev.1
-
3 (Italy, Turkey, Spain)
S19
-
1
RB51
-
0
Nationally
Internationally
Rev.1
-
2 (Spain)
S19
-
-
RB51
-
-
Official control of Brucella vaccines seed lots
(immunogenicity and residual virulence – mice model)
2
Annual reports of OIE Reference Centres, 2012
OIE RL for « Brucellosis » – « B. Garin-Bastuji » – « France »
ToR: To develop reference material in accordance with OIE requirements, and implement
and promote the application of OIE Standards.
To store and distribute to national laboratories biological reference products and any
other reagents used in the diagnosis and control of the designated pathogens or
disease.
2.
Did your laboratory produce or store imported standard reference reagents officially recognised by
the OIE or other international bodies?
Yes
3.
No
Did your laboratory supply standard reference reagents to OIE Member Countries?
Yes
Type of reagent
available
Related
diagnostic
test
No
Produced/
stored
Amount supplied
nationally
(mL, mg)
Amount supplied
internationally
(mL, mg)
National standard
panel of sera
Diagnostic
reagents
batch control
Produced
48 1 mL
-
National RBT standard
serum (BRU POS SE 01
eq. OIEISS)
RBT antigen
batch control
Produced
4 vials (1 mL)
-
National CFT/SAT/RT
standard serum (BRU
POS SE 02 eq. OIEISS)
CFT/SAT/RT
antigen batch
control
Produced
4 vials (1 mL)
-
National ELISA
standard serum
(BRU POS SE 03 eq.
OIEELISAspSS)
ELISA kits
batch control
Produced
5 vials (1 mL)
2 vials (1 mL)
National ELISA
negative standard
serum
ELISA kits
batch control
Produced
28 mL
-
Name of
recipient OIE
Member
Countries and
of institutions
International
reagent supplying
companies (France,
USA)
International
reagent supplying
company (France,)
- Estonia (4 str.)
- France (13
Departmental
Vet. Labs x 2 str.)
- Thailand (22 str.)
- EU Bacteriology
ILPT (Austria,
Brucella reference &
field strains
Brucella
conventional
identification
and typing
Brucella reference and
field strains DNAs
Brucella
molecular
identification
and typing
Stored
Produced
Annual reports of OIE Reference Laboratories, 2012
26 strains
33.5 mL
228 strains
-
Belgium, Cyprus,
Czech Republic,
Denmark, Estonia,
Finland, Germany,
Hungary, Ireland,
Italy, Latvia,
Lithuania
Netherlands,
Poland, Portugal,
Romania, Slovakia,
Slovenia UK, Spain,
Sweden (8 str. each)
- French army (1), French reagent
supplier (5)
- Estonia (2)
3
OIE RL for « Brucellosis » – « B. Garin-Bastuji » – « France »
Type of reagent
available
Related
diagnostic
test
B. melitensis Rev.1
and B. abortus S19
vaccine reference
strains
Brucella
vaccines
control
Brucellaphages
(Wb, Tb, Iz1 and R/C)
Brucella
conventional
identification
and typing
Produced/
stored
Amount supplied
nationally
(mL, mg)
Amount supplied
internationally
(mL, mg)
Name of
recipient OIE
Member
Countries and
of institutions
Stored
-
-
-
31 vials (1 mL)
- Portugal (4
phages)
- Belgium (4 phages)
- Romania (4
phages)
Produced
-
Anti-A, anti-M
monospecific, anti-R
and negative sera
Brucella
conventional
identification
and typing
Produced
78 mL
26 mL
- Belgium (3 sera), Poland ((3 sera), Romania (5 sera) Finland (3 sera)
- France (13
Departmental Vet.
Labs) (3 sera)
B. ovis antigen (CFT)
B. ovis CFT
Produced
52 mL
(104 x 0.5 mL vials)
11 mL
(22 x 0.5 mL vials)
- France (DVL)
- Ireland
- Spain
B. ovis positive control
serum (CFT)
B. ovis CFT
Produced
8.5 mL
(17 x 0.5 mL vials)
1 mL
(2 x 0.5 mL vials)
- France (DVL)
- Spain
B ovis negative control
serum (CFT)
B. ovis CFT
Produced
1 mL
(2 x 0.5 mL vials)
-
- France (DVL)
4.
Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference
reagents?
Yes
5.
No
Did your laboratory produce vaccines?
Yes
6.
No
Did your laboratory supply vaccines to OIE Member Countries?
Yes
No
ToR: To develop, standardise and validate, according to OIE Standards, new procedures for
diagnosis and control of the designated pathogens or diseases
7.
Did your laboratory develop new diagnostic methods validated according to OIE Standards for the
designated pathogen or disease?
Yes
8.
Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen
or disease?
Yes
4
No
No
Annual reports of OIE Reference Centres, 2012
OIE RL for « Brucellosis » – « B. Garin-Bastuji » – « France »
ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and
technical advice on disease control measures to OIE Member Countries
9.
Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes
No
Name of OIE
Member Country
seeking assistance
Date
(dd/mm)
Algeria
2012
Argentina
23/7/12
Nil
1 Brucella strain
Belgium
31/10/12
Nil
23 semen, 1 colostrum
EU
(Czech rep./Germany)
20/08/2012
Nil
240 porcine sera
No. samples received for
provision of diagnostic support
29 Brucella strains
4 Brucella DNA
(for molecular typing)
FYROM
10.
No. samples received for
provision of confirmatory
diagnoses
Morocco
16/02/12
Nil
122 bovine sera
UK (Great Britain)
19/4/12
Nil
2 Brucella strains
Did your laboratory provide expert advice in technical consultancies on the request of an OIE
Member Country?
Yes
No
Name of the OIE Member Country
receiving a technical consultancy
Purpose
How the advice was
provided
NIAH, Bangkok, Thailand
Implementation of reference activities in
animal brucellosis
In loco and remote
assistance
CAHEC, Qingdao, China
Belgium
Bovine brucellosis ELISA kits & B. canis
serology
Bosnia & Herzegovina
Brucella bacteriology and PCR
Croatia
Bovine brucellosis milk ELISA
Estonia
Brucella PCR
Iran
Differentiation of vaccinated from infected
animals
Northern Ireland
Official control of Bovine brucellosis ELISA
kits
Italy/Albania
Brucella Real-time PCR protocol
Kirgizstan/World Bank
Standards for Brucella vaccine control
Peru
Molecular identification of S19 vaccine strain
Portugal
Brucella biotyping – differentiation of vaccine
and field strains
Romania
Standardisation of national secondary
standard sera
Sweden
Brucella PCR protocol
Turkey
Brucella culture from milk
Annual reports of OIE Reference Laboratories, 2012
Remote assistance
5
OIE RL for « Brucellosis » – « B. Garin-Bastuji » – « France »
ToR: To carry out and/or coordinate scientific and technical studies in collaboration with
other laboratories, centres or organisations
11.
Did your laboratory participate in international scientific studies in collaboration with OIE Member
Countries other than the own?
Yes
No
ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to
the designated pathogens or diseases
12.
Did your Laboratory collect epizootiological data relevant to international disease control?
Yes
13.
No
Did your laboratory disseminate epizootiological data that had been processed and analysed?
Yes
14.
What method of dissemination of information is most often used by your laboratory?
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1.
2.
3.
4.
5.
6.
7.
8.
9.
No
a)
Articles published in peer-reviewed journals: .................
9
b)
International conferences: ..............................................
8
c)
National conferences: ......................................................
4
d)
Other: ...............................................................................
7
Scientific publications in peer-reviewed journals
Praud A, Gimenez O, Zanella G, Dufour B, Pozzi N, Antras V, Meyer L, Garin-Bastuji B, 2012. Estimation of sensitivity and
specificity of five serological tests for the diagnosis of porcine brucellosis, Prev Vet Med 104:94-100.
Praud A, Champion JL, Corde Y, Drapeau A, Meyer L, Garin-Bastuji B, 2012. Assessment of diagnostic sensitivity and
specificity of an indirect ELISA kit for the diagnosis of Brucella ovis infection in rams. BMC Vet Res 8: 68.
Mailles A, Rautureau S, Le Horgne JM, Poignet-Leroux B, d’Arnoux C, Dennetière G, Faure M, Lavigne JP, Bru JP, GarinBastuji B, 2012. Re-emergence of brucellosis in cattle in France and risk for human health. Euro Surveill 17(30).
Ferreira AC, Chambel L, Tenreiro T, Cardoso R, Flor L, Travassos Dias I, Pacheco T, Garin-Bastuji B, Le Flèche P, Vergnaud
G, Tenreiro R, Corrêa de Sá MI, 2012. MLVA16 typing of Portuguese human and animal Brucella melitensis and Brucella
abortus isolates. PLoS ONE 7(8): e42514. doi:10.1371/ journal.pone.0042514.
Rautureau S, Dufour B, Garin-Bastuji B, 2012. Maintenir la vigilance contre la brucellose bovine en France en 2011. Bull.
Epidémiol Santé Anim Alim 54:13-15.
Rautureau S, Garin-Bastuji B, Dufour B, 2012. Aucun foyer de brucellose ovine et caprine détecté en France en 2011. Bull.
Epidémiol Santé Anim Alim 54:16-19.
Marcé C, Garin-Bastuji B, 2012. Brucellose porcine en France en 2011: sept foyers dont deux en race locale. Bull.
Epidémiol Santé Anim Alim 54:41-43.
Praud A, Gimenez O, Zanella G, Dufour B, Pozzi N, Antras V, Meyer L, Garin-Bastuji B, 2012. Evaluation of five serological
tests for the diagnosis of porcine brucellosis in French Polynesia. Trop Anim Health Prod (Ahead of print DOI
10.1007/s11250-012-0309-8).
Taylor A, McGiven J, Stack J, Albert D, Banai M, Blasco JM, Fretin D, Garin-Bastuji B, Melzer F, Muñoz PM, Nicola A,
Tittarelli M, Travassos Dias I, Walravens K, Yu WL, Nielsen K, 2012. An International Standard anti-Brucella suis Serum for
Porcine Assays. Plurithematic issue – Rev Sci Tech OIE 31 (3) (In press).
6
Annual reports of OIE Reference Centres, 2012
OIE RL for « Brucellosis » – « B. Garin-Bastuji » – « France »

1.
2.
3.
4.
5.
6.
7.
8.

1.
2.
3.
4.
Presentations at international conferences and meetings
Garin-Bastuji B, 2012. Brucellosis in Cattle, Sheep & Goats: Fundamentals of surveillance and eradication programs.
Thailand-Japan Conference on Animal Health “The 25th Anniversary of National Institute of Animal Health”, Bangkok,
Thailand, May 30-31, 2012 (Invited lecture)
Garin-Bastuji B, Ekgatat M, 2102. The Brucellosis OIE Twinning Laboratory Program France-Thailand. Thailand-Japan
Conference on Animal Health “The 25th Anniversary of National Institute of Animal Health”, Bangkok, Thailand, May 3031, 2012 (communication)
Garin-Bastuji B, Ekgatat M, 2102. The Brucellosis OIE Twinning Laboratory Program France-Thailand. Thailand-Japan
Conference on Animal Health “The 25th Anniversary of National Institute of Animal Health”, Bangkok, Thailand, May 3031, 2012 (poster).
Kaewkalong S, Kanitpun R, Wongkasemjit S, Thammasart S, Ekgatat M, Garin-Bastuji B, Jirathanawat, 2012. The
Laboratory surveillance odf brucellosis in cattle, buffalo, goats and sheep in 2009-2011 in Thailand Region 1. ThailandJapan Conference on Animal Health “The 25th Anniversary of National Institute of Animal Health”, Bangkok, Thailand,
May 30-31, 2012 (poster).
Garin-Bastuji B. (2012). Diagnostic/dépistage de la Brucellose ovine & caprine - Outils éprouvés et nouveaux outils.
Réunion annuelle du Projet AUF 2011-2012 “Causes d’Avortement chez les Ruminants”, Sidi Thabet, Tunisia, September,
20-21, 2012 (communication).
Garin-Bastuji B, 2012. Key issues for the control of brucellosis in domestic ruminants. IDF World Dairy Summit, Cape
Town, South-Africa, November 4–8, 2012 (Invited lecture).
McGiven J, Stack J, Jungersen G, Garin-Bastuji B, 2012. Improving diagnostics for safer surveillance of porcine brucellosis.
5th Workshop of the EU Brucellosis NRLs – Maisons-Alfort, France, December 06-07, 2012 (communication)
Garin-Bastuji B, Corde Y, Drapeau A, Praud A, 2012. Sensitivity and specificity of C-ELISA and FPA in comparison with EU
approved tests for bovine brucellosis. 5th Workshop of the EU Brucellosis NRLs – Maisons-Alfort, France, December 06-07,
2012 (communication).
Presentations at National conferences
Garin-Bastuji B, 2012. The Brucellosis EURL - Assignments and Mandate - Specific activities. Seminario « La brucellosi tra
diagnostica e ricerca ». Teramo, Italy, October 11-12, 2012
Garin-Bastuji B, 2012. Presentation of Anses and of the Maisons-Alfort Animal Health Laboratory. EUCTP A149C3
Consultation on Standardization of reference laboratories for animal health. Qingdao, China, October 19-27, 2012.
Garin-Bastuji B, 2012. The Anses Brucellosis laboratory activities. EUCTP A149C3 Consultation on Standardization of
reference laboratories for animal health. Qingdao, China, October 19-27, 2012.
Garin-Bastuji B, 2012. Brucellosis - Epidemiological situation (World – Europe – France) - Eradication of brucellosis in
France. EUCTP A149C3 Consultation on Standardization of reference laboratories for animal health. Qingdao, China,
October 19-27, 2012.

Other
o
1.
2.
Cutler SJ, Zygmunt MS, Garin-Bastuji B, 2012. Brucella species: Brucellosis. In: M Elschner, S Cutler, M Weidmann & P
Butaye Eds, BSL3 and BSL4 Agents: Epidemiology, Microbiology, and Practical Guidelines, 1 st Edition, Wiley-VCH Verlag
GmbH & Co. KGaA, Germany, 19-36.
Olsen SC, Garin-Bastuji B, Blasco JM, Nicola AM, Samartino L, 2012. Swine Brucellosis, In: JJ Zimmerman, AL Karriker, A
Ramirez, KJ Schwartz, GW Stevenson Eds, Diseases of Swine, 10th Edition, John Wiley & Sons, USA, 697-708.
o
3.
4.
5.
6.
Consultancy and expertise reports
Collective, 2012. European Task Force for Monitoring Disease Eradication in the Member States, Report of the meeting of
the Bovine Brucellosis subgroups of the Task Force on monitoring animal disease eradication held in Newry, Northern
Ireland, UK, 06-07/12/2011, SANCO/10567/2012, 36 pp, March 2012.
Collective, 2012. Report of the meeting of the OIE ad hoc Group on Notification of Animal Diseases and Pathogenic
Agents, OIE, Paris 24-26 July 2012.
Collective, 2012. European Task Force for Monitoring Disease Eradication in the Member States, Report of the meeting of
the Brucellosis subgroup of the Task Force on monitoring animal disease eradication held in Halkidiki, Greece, 1415/06/2012, SANCO/12350/2012, 27 pp, October 12.
Collectif, 2011. Rapport et Projet d'avis de l’Anses relatif à la survie de Brucella dans les produits laitiers. Saisine Anses
2010-SA-00115, November 2012.
o
7.
Book chapters
PhD thesis
Praud A., 2012. Apport de l’épidémiologie dans le choix des outils d’aide à la prise de décision sanitaire en santé animale.
Evaluation des tests de dépistage en santé animale. Université Paris XI, Faculté de Médecine Paris-Sud, Ecole doctorale
420, Santé Publique, Paris Sud 11, Paris Descartes.
Annual reports of OIE Reference Laboratories, 2012
7
OIE RL for « Brucellosis » – « B. Garin-Bastuji » – « France »
ToR: To provide scientific and technical training for personnel from OIE Member Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
15.
Did your laboratory provide scientific and technical training to laboratory personnel from other OIE
Member Countries?
Yes
No
a)
Technical visits: ................................................................ 8
b)
Seminars: ......................................................................... 0
c)
Hands-on training courses: .............................................. 18
d)
Internships (>1 month): ................................................... 1
Type of technical training
provided (a, b, c or d)
Country of origin of the expert(s)
provided with training
No. participants from the
corresponding country
a and c
Thailand
4
a
China
4
c
EU CFT training course (Austria, Belgium,
Estonia, Poland, Romania, Sweden)
6
c
EU Bacteriology training course (Denmark,
Finland, Italy, Latvia, Lithuania, Poland,
Romania, Spain)
8
d
Algeria
1
ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the
pathogen and the disease concerned
16.
Does your laboratory have a Quality Management System certified according to an International
Standard?
Yes
No
Quality management system adopted
ISO 17025
17.
Is your laboratory accredited by an international accreditation body?
Yes
Test for which your laboratory is accredited
No
Accreditation body
RBT / CFT /SAT / MRT / ELISA
Isolation, identification and biotyping of Brucella
Control of RBT, CFT, SAT, MRT antigens & ELISA kits
COFRAC
(member of EA and ILAC)
Control of Brucella vaccines
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease
concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or
Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
8
Annual reports of OIE Reference Centres, 2012
OIE RL for « Brucellosis » – « B. Garin-Bastuji » – « France »
Yes
No
ToR: To organise and participate in scientific meetings on behalf of the OIE
19.
Did your laboratory organise scientific meetings on behalf of the OIE?
Yes
20.
No
Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes
No
ToR: To establish and maintain a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organise regular inter-laboratory
proficiency testing to ensure comparability of results
21.
Did your laboratory exchange information with other OIE Reference Laboratories designated for the
same pathogen or disease?
Yes
22.
No
Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated
for the same pathogen or disease by organising or participating in proficiency tests?
Yes
23.
No
Did your laboratory collaborate with other OIE Reference Laboratories for the same disease
on scientific research projects for the diagnosis or control of the pathogen of interest?
Yes
No
Title of the project or contact
Scope
Name(s) of relevant OIE
Reference Laboratories
Improving diagnostics for safer
surveillance of porcine
brucellosis.
Club V AHVLA/Anses/DTU
project
Develop specific serological tests for porcine
brucellosis
AHVLA, Weybridge (UK)
ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results
Annual reports of OIE Reference Laboratories, 2012
9
OIE RL for « Brucellosis » – « B. Garin-Bastuji » – « France »
24.
Did your laboratory organise or participate in inter-laboratory proficiency tests with
laboratories other than OIE Reference Laboratories for the same disease?
Yes (organiser)
No
No. participating
laboratories
Participating OIE Member
Countries
109
France, all EU Member states, Albania,
Argentina, Bosnia and Herzegovina,
Croatia, FYROM, Kosovo, Montenegro,
Norway, Serbia, Switzerland, Thailand,
Turkey
Brucellosis serology (milk ELISA)
18
France, Austria, Croatia, Cyprus, Czech
Rep., Estonia, Germany, Greece,
Ireland, Italy, Latvia, Lithuania, Malta,
Poland, Romania, Spain, Sweden, UK
(Great-Britain), UK (Northern Ireland)
Brucella bacteriology
24
All EU Member states but Bulgaria and
Malta
Purpose for inter-laboratory test comparisons1
Brucellosis serology (blood tests)
ToR: To place expert consultants at the disposal of the OIE
25.
Did your laboratory place expert consultants at the disposal of the OIE?
Yes
No
Kind of consultancy
Location
Subject (facultative )
Ad hoc Group
Paris
Notification of Animal Diseases and
Pathogenic Agents
Reviewing / Expert panel
Paris
Procedure for OIE Validation and
Certification of Diagnostic Assays
_______________
1
10
See Interlaboratory test comparisons in: Laboratory Proficiency Testing at:
www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3
Annual reports of OIE Reference Centres, 2012