Molecular Biology Services
Plasmid Preparation Submission Form
1st BASE Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs-sg@base-asia.com
First BASE Laboratories Sdn Bhd (604944-X)
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
*Required fields
CUSTOMER DETAILS
*Name:
Reference ID:
Previous PCR Optimization Reference ID (if any)
*Institute Name / Department
*Address:
*Contact Number:
*Email Address:
Principal Investigator / Supervisor:
(Office)
(Mobile)
Type of Service Required
Please indicate with a “X”
Services
Miniprep
Midiprep
Maxiprep
Page 1 of 3
Molecular Biology Services
Plasmid Preparation Submission Form
1st BASE Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs-sg@base-asia.com
^Increase number of rows if necessary, or attach a separate sheet
*Please fill in where applicable
^PLASMID DETAILS
S/N
Sample Name
High Copy (HC) /
Concentration Absorbance
Low Copy (LC)
(ng/µl)
ratio
plasmid
(A260/280)
1.
2.
3.
4.
5.
6.
Antibiotic
resistance
First BASE Laboratories Sdn Bhd (604944-X)
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
Volume
submitted
(µl)
#
Restriction digestion
verification with:
Linearized size
(bp)
7.
8.
9.
10.
# We provide “BamHI, EcoRI, HindIII, NotI, SalI, XhoI, EcoRV” at no additional charge.
ADDITIONAL SERVICES (Additional fees are applicable)
Quantity
Restriction Digestion Verification of Plasmid
Stab Culture
Services
Glycerol Stock
Bi-directional Sequencing of Cloning Sites
Note:
1. 1st BASE will perform quality checks on samples upon receipt.
2. Final plasmid construct will be delivered as 10 g (miniprep), ≥ 50 g (midiprep), and ≥ 100 g (maxiprep) as lyophilized DNA. Service will also include Service Report.
Page 2 of 3
Molecular Biology Services
Plasmid Preparation Submission Form
1st BASE Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs-sg@base-asia.com
First BASE Laboratories Sdn Bhd (604944-X)
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
Have I met the sample preparation requirements?
Checklist (Please tick √):
Submitted ≥ 100 ng* of plasmid in either TE or 10mM Tris-HCl (pH8.0) Elution Buffer
≥ 500 ng is required if “Restriction Digestion Verification of Plasmid” is needed.
Purified DNA meets OD260/280= 1.8 to 2.0
Attached Gel Photo -100 ng plasmid digested by restriction enzyme and its control without digestion. Please indicate DNA ladder, control (undigested
plamid), digested plasmid. Without gel photo, 1st BASE will perform “Restriction Digestion Verification of Plasmid” (charge is applied).
Samples submitted in 1.5ml microcentrifuge tubes with at least 10 µl of contents. Each tube should be clearly labeled using a permanent marker, with caps
sealed with parafilm. DNA should be shipped chilled.
Page 3 of 3