BIO226: Laboratory Safety
BIO226:
Laboratory Safety
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Chemicals in the Laboratory
Biological /Biosafety Containment
Disposal of Laboratory Waste
Working with Radioactive Materials
Biosafety Laboratories
Small Animal Handling
Safety in the Laboratory
The lab portion of BIO225 Laboratory Safety course will enable the student to
develop what is known in industry as gLP or defined good Laboratory Practice.
This will include the secure use and handling of biological and chemical and
radiological materials in a laboratory setting. Topics covered will include
safety procedures in Biosafety Level 2 and 3 labs, safety with infectious disease,
safe handling of hazardous biological and chemical materials and safe
practices with small animals in laboratory settings. At the end of the course,
students should be able to demonstrate safe practice in various lab types.
Required Materials: No required test
Strongly suggested text:
Seidman, Moore. (2009) Basic Laboratory
Methods for Biotechnology. 2nd. San Francisco, CA: Pearson
Learning Outcomes for This Lab Course
The intention is for the student to be able to:
1. Follow basic safety guidelines when working in a laboratory.
2. Safely work with and store chemicals in the laboratory.
3. Demonstrate successful work with biological and biosafety
containment equipment.
4. Explain how to work with radioactive materials.
5. Demonstrate proper methods of disposal for the different types of
laboratory generated waste.
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Advanced Biotechnology Certificate Instructional Laboratory Manual
6. Perform appropriately within the different Biosafety level labs .
7. Demonstrate understanding of the requirements of receiving and
shipping of samples safely.
8. Demonstrate proper handling of small animals in the laboratory for
research purposes.
Course Competencies (Skills)
Actions that are essential to achieve the learning outcomes:
1. Practice defined gLP during laboratory sessions
2. Reduce and defuse potential hazards in the laboratory in a manner
consistent with the appropriate safety level
3. Classify, using a variety of sources, the chemical properties of the
chemicals found in a lab.
4. Properly store and label different classes of chemicals, including
categorizing and using correct containment areas.
5. Use defined Personal Protective Equipment consistent with the mode
of entry of bacteria, virus, mold and fungi.
6. Exhibit a working knowledge of proper procedures when working with
biological, chemical and radiological materials.
7. Design procedures for minimizing and disposing of lab generated
wastes.
8. Explain and demonstrate cleanup of specific types of spills (acids,
caustics agents, volatile chemicals) in the lab
9. Choose the correct type of Biological Safety Cabinet (according to
function of each) for specific laboratory procedures.
10. Successfully handle and inject small laboratory animals for various
research situations.
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Advanced Biotechnology Certificate Satellite Laboratory Manual