1
SUPPORTING INFORMATION
2
Results - Biological measurements against developmental time
3
Mortality
4
Mortality was significantly correlated to developmental time in most cultures, except from two
5
cultures (Table S8; p < 0.05). Mortality rates were low (< 5% day-1) and similar at pHT ≥ 6.90
6
(Fig. S1, linear regression, p = 0.77, n = 22). For the lowest pH cultures (pHT ≤ 6.50), mortality
7
was nearly two times higher than in the rest of the pHs.
8
Morphology
9
Growth rates of the four arms (POL, AL, PDL and PRL) were calculated from the significant
10
linear relationship between the arm length and developmental time (Tables S9 A-D; p < 0.05)
11
and plotted against mean pHT (Fig. S2). The growth rates of arms were significantly lowered
12
when pHT was decreased (POL: p = 0.002, R2 = 37 %, n = 24; AL: p = 0.001, R2 = 41%, n = 23;
13
PDL: p = 0.002, R2 = 43%, n = 20), except from the PRL (p = 0.24, n = 9).
14
Both larval body width (BW) and paired arm tip distance (AG, POG, PDG) growth rates
15
were calculated from the significant linear relationship between these measures and
16
developmental time (Tables S10 A-D; p < 0.05). Neither BW, AG nor POG growth rates showed
17
a significant relationship to mean pHT (Fig. S3; p > 0.05, n = 24, 21 and 23 respectively).
18
Although PDG displayed a significant relationship with mean pHT (p < 0.01), this relation was
19
considered biased since only four cultures had significant PDG growth rates (Tables S10 D).
20
Growth rates of the stomach volume were calculated from the significant linear
21
relationship between the stomach volume (SV) and developmental time (Table S11; p < 0.05).
22
This SV growth showed no significant relationship to mean pHT (Fig. S4; p = 0.53, n = 24).
23
Respiration
24
Respiration rates were calculated from the significant linear relationship between the respiration
25
and developmental time (Table S12; p < 0.05) and plotted against mean pHT (Fig. S5).
26
Respiration rates were not significantly correlated with pHT (p = 0.41, n = 18).
27
Developmental stages
28
At days 1, 2, 3, 5, 7, 9, 11, 13,15 and 24, individuals were stages as gastrula (G) or pluteus (four
29
to eight arms: P4, P6 and P8). The proportion of each stage was calculated for 6 pH categories
30
(pHT 8.0, 7.8. 7.6, 7.4, 7.2 and 7.0). The duration of G, P4 and P6 development stages (stage
31
duration) was calculated as the difference of the median development time of two successive
32
stages (the time when 50% of the organisms in a culture had passed that stage, which in turn was
33
estimated from stage–frequency data converted to stage proportion). The stage duration for
34
gastrula was 2.6±0.6 days with no effect of pH (Fig. S6) except for pHT=7.0 with a stage
35
duration of 4.5 days. Stage duration increased significantly with decreasing pH for both P4
36
(p<0.01) and P6 (p<0.01) stages (Fig. S6).
37
SUPPLEMENTARY FIGURES CAPTIONS:
38
Fig. S1: Relationship between larval mortality rate per developmental time (% larvae day-1) and
39
pHT. Each dot represents the regression coefficient extracted from the significant linear
40
relationship between mortality and time, for each culture (see regressions in Table S8).
41
42
Fig. S2: Relationship between arm (POL: postoral; AL: anterolateral; PDL: posterodorsal; PRL:
43
preoral) growth rate per developmental time (μm day-1) and pHT. Each dot represents the
44
regression coefficient extracted from the significant linear relationship between arm length and
45
time, for each culture (see regressions in Tables S9A-D).
46
47
Fig. S3: Relationship between vertical gaps (BW: body width; POG: postoral arms gap; AG:
48
anterolateral arms gap; PDG: postdorsal arm gap) growth rate per developmental time (μm day-1)
49
and pHT. Each dot represents the regression coefficient extracted from the significant linear
50
relationship between gap length and time, for each culture (see regressions in Tables S10A-D).
51
52
Fig. S4: Relationship between stomach volume (SV) growth rate per developmental time (μm3
53
day-1) and pHT. Each dot represents the regression coefficient extracted from the significant
54
linear relationship between SV and time, for each culture (see regressions in Table S11).
55
56
Fig. S5: Relationship between respiration rate per developmental time (pmol O2 h-1 larva-1 day-1)
57
and pHT. Each dot represents the regression coefficient extracted from the significant linear
58
relationship between respiration and time, for each culture (see regressions in Tables S12).
59
60
Fig. S6: Relationship between stage duration (day) for gastrula (G), pluteus-4 (P4) and pluteus-6
61
(P6) developmental stages and pHT category.
62
SUPPLEMENTARY FIGURES:
Fig. S1:
Fig. S2:
Fig. S3:
Fig. S4:
Fig. S5:
Fig. S6:
1
SUPPLEMENTARY TABLES CAPTIONS:
2
Table S1: Larval growth rates (GR BL: μm day-1) were calculated as the coefficient of the
3
significant logarithmic relationship between BL and time. Results of the regressions (Intercept,
4
p-value, R2, F-value and df: degree of freedom) are given for each culture replicate (R) with
5
corresponding average pHT (pHT). Data in bold (p-value>0.05) were removed from subsequent
6
analyses.
7
8
Table S2: Larval mortality rate (% larvae μmBL-1) were calculated as the coefficient of the
9
significant linear relationship between mortality and BL. Results of the regressions (Intercept, p-
10
value, R2, F-value and df: degree of freedom) are given for each culture replicate (R) with
11
corresponding average pHT (pHT). Data in bold (p-value>0.05) were removed from subsequent
12
analyses.
13
14
Table S3A-D: Larval arms growth rates (μm μmBL-1) of the four arm pairs (A. GR POL:
15
postoral; B. GR AL: anterolateral; C. GR PDL: posterodorsal; D. GR PRL: preoral) were
16
calculated as the coefficient of the significant linear relationship between the longest arm length
17
and BL. Results of the regressions (Intercept, p-value, R2, F-value and df: degree of freedom) are
18
given for each culture replicate (R) with corresponding average pHT (pHT). Data in bold (p-
19
value>0.05) were removed from subsequent analyses. Only replicates with enough data available
20
(n>4) to perform a regression are presented.
21
22
Table S4A-D: Larval arms’ symmetry (no units) of the four arm pairs (A. Symmetry POL:
23
postoral; B. Symmetry AL: anterolateral; C. Symmetry PDL: posterodorsal; D. Symmetry PRL:
24
preoral) were calculated as the coefficient of the significant linear relationship between the
25
longest and the shortest arm length of each pair, with null intercept. Results of the regressions (p-
26
value, R2, F-value and df: degree of freedom) are given for each culture replicate (R) with
27
corresponding average pHT (pHT). Only replicates with enough data available (n>4) to perform a
28
regression are presented.
29
30
Table S5A-D: Larval width (A. GR BW: body width) and gaps (B. GR POL: postoral; C. GR
31
AL: anterolateral; D. GR PDL: posterodorsal) growth rates (μm μmBL-1) of the four arm pairs
32
were calculated as the coefficient of the significant linear relationship between the length of each
33
measure and BL. Results of the regressions (Intercept, p-value, R2, F-value and df: degree of
34
freedom) are given for each culture replicate (R) with corresponding average pH T (pHT). Data in
35
bold (p-value>0.05) were removed from subsequent analyses. Only replicates with enough data
36
available (n>4) to perform a regression are presented.
37
38
Table S6: Stomach volume growth rates (GR SV: μm3 μmBL-1) were calculated as the coefficient
39
of the significant linear relationship between the stomach volume and BL. Results of the
40
regressions (Intercept, p-value, R2, F-value and df: degree of freedom) are given for each culture
41
replicate (R) with corresponding average pHT (pHT). Data in bold (p-value>0.05) were removed
42
from subsequent analyses. Only replicates with enough data available (n>4) to perform a
43
regression are presented.
44
45
Table S7: Respiration rates (pmol O2 h-1 larvae-1 μmBL-1) were calculated as the coefficient of
46
the significant linear relationship between the respiration and BL. Results of the regressions
47
(Intercept, p-value, R2, F-value and df: degree of freedom) are given for each culture replicate
48
(R) with corresponding average pHT (pHT). Data in bold (p-value>0.05) were removed from
49
subsequent analyses. Only replicates with enough data available (n>4) to perform a regression
50
are presented.
51
52
Table S8: Larval mortality rate (% larvae day-1) were calculated as the coefficient of the
53
significant linear relationship between mortality and time. Results of the regressions (Intercept,
54
p-value, R2, F-value and df: degree of freedom) are given for each culture replicate (R) with
55
corresponding average pHT (pHT). Data in bold (p-value>0.05) were removed from subsequent
56
analyses.
57
58
Tables S9A-D: Larval arms growth rates (μm day-1) of the four arm pairs (A. GR POL: postoral;
59
B. GR AL: anterolateral; C. GR PDL: posterodorsal; D. GR PRL: preoral) were calculated as the
60
coefficient of the significant linear relationship between the longest arm length and time. Results
61
of the regressions (Intercept, p-value, R2, F-value and df: degree of freedom) are given for each
62
culture replicate (R) with corresponding average pHT (pHT). Data in bold (p-value>0.05) were
63
removed from subsequent analyses. Only replicates with enough data available (n>4) to perform
64
a regression are presented.
65
66
Table S10A-D: Larval width (A. GR BW: body width) and gaps (B. GR POL: postoral; C. GR
67
AL: anterolateral; D. GR PDL: posterodorsal) growth rates (μm day-1) of the four arm pairs were
68
calculated as the coefficient of the significant linear relationship between the length of each
69
measure and time. Results of the regressions (Intercept, p-value, R2, F-value and df: degree of
70
freedom) are given for each culture replicate (R) with corresponding average pH T (pHT). Data in
71
bold (p-value>0.05) were removed from subsequent analyses. Only replicates with enough data
72
available (n>4) to perform a regression are presented.
73
74
Table S11: Stomach volume growth rates (GR SV: μm3 day-1) were calculated as the coefficient
75
of the significant linear relationship between the stomach volume and time. Results of the
76
regressions (Intercept, p-value, R2, F-value and df: degree of freedom) are given for each culture
77
replicate (R) with corresponding average pHT (pHT). Only replicates with enough data available
78
(n>4) to perform a regression are presented.
79
80
Table S12: Respiration rates (pmol O2 h-1 larvae-1 day-1) were calculated as the coefficient of the
81
significant linear relationship between the respiration and time. Results of the regressions
82
(Intercept, p-value, R2, F-value and df: degree of freedom) are given for each culture replicate
83
(R) with corresponding average pHT (pHT). Data in bold (p-value>0.05) were removed from
84
subsequent analyses. Only replicates with enough data available (n>4) to perform a regression
85
are presented.
86
SUPPLEMENTARY TABLES:
87
Table S1
88
R
pHT
GR BL
Intercept
p-value
R2
F-value
df
6.5a
6.5b
6.5c
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
6.44
6.50
6.45
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
-5.5
7.1
-3.5
39
38
73
84
83
72
66
69
95
89
94
84
92
93
87
96
88
85
101
94
103
93
100
91
164
156
159
162
164
149
148
144
146
171
165
139
149
147
159
147
148
159
150
158
166
148
158
146
158
152
159
3.1E-01
4.7E-02
4.0E-01
2.2E-14
1.2E-13
4.8E-35
1.6E-40
7.6E-41
4.0E-39
2.0E-30
1.3E-35
2.5E-44
2.3E-45
4.4E-39
1.2E-40
4.1E-43
9.8E-41
2.2E-35
2.7E-50
7.4E-52
2.5E-47
5.8E-38
5.2E-34
3.4E-48
1.9E-44
7.5E-53
6.6E-54
0.04
0.09
0.02
0.47
0.52
0.87
0.90
0.92
0.87
0.77
0.87
0.94
0.93
0.88
0.88
0.91
0.91
0.89
0.94
0.92
0.88
0.91
0.91
0.95
0.93
0.95
0.93
1.6
4.5
1.0
85
92
582
836
982
675
347
563
1396
1065
743
683
899
821
666
1373
1184
798
864
678
1516
1111
1658
1300
43
48
44
94
84
90
98
88
104
104
88
96
86
102
96
89
86
80
90
98
106
81
71
87
85
92
99
89
90
TableS2
R
pHT
6.5a
6.5b
6.5c
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
6.44
6.50
6.45
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
Mortality
rates
-18.4
11.6
-28.7
0.85
0.99
0.51
0.36
0.36
0.10
0.33
0.54
0.33
0.23
0.14
0.41
0.22
0.36
0.38
0.29
-0.01
0.15
0.36
0.43
0.31
0.36
0.32
0.34
Intercept
p-value
R2
F-value
df
2903
-1922
4447
-247
-280
-207
-178
-171
-94
-164
-214
-162
-138
-118
-199
-147
-181
-177
-162
-53
-120
-186
-202
-162
-169
-167
-170
6.7E-06
1.8E-03
7.3E-04
3.6E-11
1.1E-09
3.9E-06
4.7E-05
2.0E-04
1.6E-01
7.1E-04
6.9E-06
2.2E-05
8.6E-03
2.5E-03
1.2E-04
6.0E-03
8.2E-06
6.2E-05
2.2E-04
8.7E-01
1.8E-02
9.3E-06
1.2E-05
4.1E-05
8.5E-06
7.4E-05
2.8E-05
0.91
0.68
0.74
0.91
0.86
0.68
0.59
0.53
0.10
0.46
0.66
0.62
0.31
0.39
0.55
0.34
0.66
0.58
0.52
0.001
0.26
0.65
0.64
0.60
0.66
0.57
0.61
85.8
19.2
25.1
181.7
120.9
40.9
27.4
21.1
2.17
16.3
37.5
31.3
8.59
12.1
23.2
9.57
36.5
26.1
20.8
0.03
6.70
35.8
34.3
28.1
36.3
25.3
29.9
9
9
9
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
91
92
Table S3A
R
pHT
GR POL
Intercept
p-value
R2
F-value
df
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
1.3
0.9
1.7
1.9
2.0
1.8
1.2
1.6
2.0
2.6
1.5
1.9
3.0
2.1
2.4
2.6
2.1
2.5
2.6
2.7
3.0
2.9
3.5
2.8
-42
39
-149
-232
-257
-189
-20
-122
-226
-444
-80
-197
-568
-260
-364
-423
-263
-425
-434
-448
-558
-508
-708
-471
2.4E-12
1.3E-07
1.1E-14
1.3E-20
2.2E-21
1.8E-16
3.6E-11
1.2E-14
1.7E-19
2.2E-16
1.7E-20
2.0E-16
2.4E-26
3.0E-16
2.4E-17
9.4E-22
7.0E-16
4.3E-22
8.8E-18
1.3E-13
6.5E-22
4.0E-21
1.9E-26
3.9E-25
0.65
0.53
0.65
0.73
0.80
0.66
0.45
0.63
0.72
0.70
0.71
0.64
0.85
0.71
0.76
0.79
0.62
0.71
0.77
0.74
0.81
0.80
0.84
0.79
88.12
42.37
106.8
179.4
223.8
123.3
60.61
102.3
166.9
132.4
169.7
119.5
345.7
134.1
162.7
220.4
110.3
185.6
168.4
117.5
235.8
225.8
327.9
260.7
47
37
57
67
57
63
73
61
64
56
71
66
59
54
50
60
68
76
51
41
57
55
62
69
93
Table S3B
R
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
94
pHT
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR AL
0.28
0.14
0.76
0.69
0.75
0.82
0.46
0.73
1.13
1.23
0.64
0.88
1.44
0.94
1.35
1.36
1.23
1.60
1.53
1.50
1.54
1.71
1.73
1.54
Intercept
-30.6
-8.29
-168
-152
-170
-200
-112
-172
-273
-324
-132
-202
-386
-231
-348
-363
-323
-453
-420
-401
-427
-460
-474
-401
p-value
2.9E-03
6.5E-02
9.2E-13
1.3E-15
2.0E-13
4.1E-09
4.5E-06
2.0E-10
5.0E-20
1.2E-13
6.5E-10
3.3E-10
1.5E-17
8.4E-18
7.0E-17
5.8E-17
1.3E-12
1.2E-27
8.6E-21
1.7E-14
2.2E-19
4.0E-16
5.1E-20
2.3E-20
R2
0.22
0.11
0.59
0.62
0.62
0.44
0.25
0.49
0.73
0.63
0.42
0.45
0.71
0.75
0.75
0.69
0.53
0.79
0.82
0.77
0.76
0.70
0.74
0.71
F-value
10.18
3.67
83.5
109.2
91.1
47.2
24.6
58.0
175.9
94.8
51.2
54.5
145.4
160.4
153.9
134.5
75.3
289.6
236.6
133.8
181.9
130.3
180.5
171.2
df
37
30
57
66
57
60
72
61
64
56
70
66
59
54
50
60
68
76
51
41
57
55
62
69
95
Table S3C
R
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
96
pHT
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR PDL
1.00
0.27
0.89
0.73
0.72
0.91
0.22
-0.25
1.39
1.13
0.81
1.32
0.57
0.55
0.84
0.77
1.43
1.32
1.63
0.97
1.50
1.27
1.65
1.41
Intercept
-236
0.36
-212
-152
-109
-172
23.7
176
-366
-285
-172
-336
-54.6
-71.1
-156
-130
-355
-354
-508
-235
-430
-346
-498
-388
p-value
1.2E-03
6.8E-01
1.5E-03
4.0E-08
0.038
4.9E-03
3.2E-01
5.0E-01
7.4E-08
1.9E-05
9.7E-09
2.2E-04
2.1E-01
5.5E-02
1.7E-03
2.3E-02
6.9E-05
6.0E-05
3.4E-10
3.7E-05
7.0E-08
7.5E-05
1.3E-07
1.1E-08
R2
0.63
0.07
0.55
0.79
0.40
0.36
0.04
0.03
0.66
0.65
0.66
0.39
0.08
0.19
0.49
0.26
0.44
0.35
0.79
0.66
0.65
0.49
0.62
0.55
F-value
18.9
0.21
16.1
73.4
5.93
10.3
1.02
0.58
53.4
32.9
60.0
18.0
1.66
4.22
14.5
6.19
21.8
20.4
95.6
31.8
52.4
22.7
48.1
50.7
df
11
3
13
20
9
18
22
17
27
18
31
28
18
18
15
18
28
38
26
16
28
24
29
41
97
98
Table S3D
R
pHT
GR PRL
Intercept
p-value
R2
F-value
df
7.3a
7.3b
7.3c
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
7.25
7.19
7.24
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
0.014
-0.030
0.184
0.605
0.590
0.055
0.418
0.284
-0.037
0.033
0.152
0.032
0.553
1.021
0.214
0.061
0.045
0.208
0.009
0.775
15.9
34.6
-30.6
-185
-184
0.32
-154
-90.8
38.5
2.46
37.9
8.52
-186
-383
-70.2
-2.02
5.55
-62.3
26.4
-277
8.6E-01
7.1E-01
3.9E-01
1.8E-01
7.8E-07
6.3E-01
2.6E-02
5.1E-03
7.9E-01
5.8E-01
8.5E-03
7.4E-01
1.6E-02
3.9E-03
1.1E-02
7.9E-01
6.4E-01
9.4E-03
9.3E-01
5.3E-05
<0.01
0.02
0.05
0.40
0.77
0.04
0.38
0.44
0.01
0.04
0.40
0.01
0.22
0.29
0.38
0.01
0.01
0.39
<0.01
0.41
0.03
0.14
0.77
2.71
57.1
0.26
6.62
11.0
0.07
0.34
9.36
0.11
6.78
10.1
8.71
0.07
022
9.06
0.01
22.0
13
8
14
4
17
7
11
14
7
8
14
14
24
25
14
10
17
14
18
31
99
100
Table S4A
R
pHT
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
Symmetry
POL
0.88
0.91
0.92
0.92
0.92
0.91
0.93
0.93
0.93
0.95
0.94
0.93
0.94
0.95
0.95
0.95
0.93
0.94
0.95
0.94
0.95
0.97
0.95
0.96
p-value
R2
F-value
df
3.5E-45
3.3E-44
2.8E-65
1.9E-70
5.2E-63
6.6E-67
7.4E-94
1.4E-81
3.5E-79
2.3E-74
1.3E-98
1.5E-86
2.2E-78
3.7E-71
1.8E-66
4.3E-84
8.3E-76
2.3E-96
2.8E-70
3.0E-57
1.5E-82
7.9E-89
2.2E-74
9.6E-106
0.98
0.99
0.99
0.99
0.99
0.99
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
0.99
1.00
1.00
1.00
1.00
1.00
1.00
1.00
3075
6596
8970
7070
7480
6904
22747
23296
15642
20157
35231
22723
21521
18414
17684
30558
9607
21972
22559
18654.47
35551
72380
12736
65463
48
38
58
68
58
64
74
62
65
57
72
67
60
55
51
61
69
77
52
42
58
56
63
70
101
102
Table S4B
R
pHT
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
Symmetry
AL
0.75
0.69
0.80
0.84
0.84
0.84
0.73
0.86
0.85
0.86
0.88
0.86
0.92
0.89
0.91
0.93
0.88
0.92
0.92
0.91
0.93
0.87
0.93
0.93
p-value
R2
F-value
df
1.4E-25
2.3E-19
3.0E-45
2.0E-46
5.9E-46
8.3E-49
5.9E-50
3.0E-59
5.2E-54
3.8E-45
7.5E-72
1.4E-58
6.4E-70
6.7E-56
3.2E-53
1.2E-70
8.0E-72
1.1E-79
1.2E-58
2.3E-43
8.9E-69
6.6E-38
2.1E-77
4.4E-86
0.95
0.93
0.97
0.95
0.97
0.97
0.95
0.99
0.98
0.97
0.99
0.98
0.99
0.99
0.99
0.99
0.99
0.99
0.99
0.99
1.00
0.95
1.00
1.00
657
406
1784
1379
1889
2074
1455
4387
2575
1850
6628
3269
11206
5092
5307
11035
7346
8063
8019
4034
11858
1045
15854
17912
38
31
58
67
58
61
73
62
65
57
71
67
60
55
51
61
69
77
52
42
58
56
63
70
103
104
Table S4C
R
pHT
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
Symmetry
PDL
0.68
0.55
0.60
0.70
0.78
0.83
0.75
0.79
0.80
0.87
0.79
0.86
0.85
0.78
0.78
0.88
0.85
0.83
0.82
0.85
0.88
0.87
0.91
0.87
p-value
R2
F-value
df
7.0E-07
5.6E-03
5.4E-05
1.8E-11
1.5E-11
1.7E-16
4.0E-14
1.5E-13
1.6E-21
3.1E-20
1.7E-25
4.6E-30
1.4E-16
1.1E-14
3.3E-14
9.5E-21
7.4E-21
5.0E-34
1.2E-20
1.4E-13
4.0E-24
8.1E-24
7.0E-29
1.5E-38
0.88
0.88
0.70
0.89
0.99
0.97
0.92
0.95
0.96
0.99
0.97
0.99
0.97
0.96
0.97
0.99
0.95
0.98
0.96
0.96
0.97
0.98
0.98
0.98
88.25
29.46
32.65
167.7
1090
706.2
265.5
379.2
721.2
1775
968.4
2653
720.8
449.2
617.5
2014
593.5
1752
676.6
438.0
1016
1493
1958
2366
12
4
14
21
10
19
23
18
28
19
32
29
19
19
16
19
29
39
27
17
29
25
30
42
105
106
Table S4D
R
pHT
7.3a
7.3b
7.3c
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
7.25
7.19
7.24
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
Symmetry
PRL
0.64
0.74
0.70
0.86
0.89
0.60
0.70
0.87
0.73
0.76
0.73
0.73
0.83
0.91
0.72
0.80
0.80
0.73
0.80
0.85
p-value
R2
F-value
df
9.1E-09
4.0E-07
7.7E-12
3.7E-08
8.1E-20
2.5E-04
8.1E-11
2.0E-12
1.6E-06
6.9E-07
2.5E-12
9.3E-12
5.0E-22
4.6E-31
4.5E-09
1.2E-10
3.8E-15
7.4E-12
4.5E-14
1.1E-28
0.91
0.95
0.96
1.00
0.99
0.83
0.97
0.97
0.95
0.94
0.97
0.96
0.98
0.99
0.91
0.98
0.97
0.96
0.95
0.98
144.5
167.6
353.4
3051
1958
38.75
439.4
426.1
155.8
148.0
414.1
344.2
1067
4832
143.0
526.8
580.9
355.4
384.8
1553
14
9
15
5
18
8
12
15
8
9
15
15
25
26
15
11
18
15
19
32
107
Table S5A
R
6.5a
6.5b
6.5c
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
108
pHT
6.44
6.50
6.45
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR BW
0.63
0.89
0.64
0.17
0.03
0.45
0.55
0.52
0.59
0.39
0.34
0.54
0.44
0.48
0.45
0.44
0.40
0.39
0.40
0.45
0.45
0.40
0.33
0.35
0.26
0.39
0.38
Intercept
43.68
11.00
50.04
138.9
170.5
88.30
68.79
70.37
64.17
106.9
114.9
65.39
90.83
78.23
86.39
90.48
102.8
105.5
98.87
92.14
86.04
102.8
117.4
114.8
131.9
103.9
103.3
p-value
4.5E-06
9.3E-09
7.4E-08
3.4E-03
6.9E-01
2.2E-17
5.0E-29
2.7E-20
7.9E-26
1.7E-14
2.8E-12
6.4E-35
2.2E-23
6.8E-28
3.4E-28
4.1E-27
2.5E-24
4.1E-19
9.8E-26
2.1E-26
5.5E-22
4.2E-21
4.6E-17
3.6E-21
3.0E-10
3.9E-22
5.9E-25
R2
0.39
0.50
0.49
0.09
0.00
0.55
0.72
0.62
0.66
0.43
0.43
0.80
0.69
0.69
0.72
0.73
0.70
0.63
0.71
0.69
0.59
0.67
0.63
0.64
0.37
0.64
0.66
F-value
27.5
48.1
41.6
9.03
0.16
111
255
145
198
79.6
65.7
374.8
188
230
245
242
203
138
217
214
150
163
122
157
51.0
164
193
df
43
48
44
92
84
90
98
88
104
104
88
96
86
102
96
89
86
80
90
98
106
81
71
87
85
92
99
109
Table S5 B
R
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
110
pHT
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR POG
1.09
1.20
0.64
0.96
1.00
1.47
0.71
0.74
0.85
1.63
0.93
1.10
1.47
1.03
0.86
1.76
1.37
1.69
0.98
0.57
1.14
0.31
1.23
0.50
Intercept
-99.06
-116.6
43.53
-89.45
-107.7
-182.2
-23.93
-22.91
-21.91
-289.6
-68.03
-91.78
-228.1
-100.6
-55.50
-354.4
-208.2
-322.9
-73.92
55.84
-146.9
138.0
-169.1
93.59
p-value
8.4E-06
3.2E-05
1.3E-03
7.7E-08
3.7E-08
6.2E-13
5.8E-08
1.1E-06
2.4E-06
1.3E-11
1.3E-12
1.5E-08
1.1E-09
9.0E-09
3.2E-05
1.8E-14
4.9E-13
1.2E-16
2.8E-09
1.3E-03
7.5E-09
1.0E-01
2.2E-09
6.8E-03
R2
0.39
0.43
0.18
0.36
0.41
0.57
0.34
0.32
0.30
0.56
0.51
0.39
0.47
0.46
0.30
0.63
0.54
0.60
0.50
0.23
0.45
0.05
0.44
0.10
F-value
26.1
23.7
11.5
36.7
40.4
81.8
36.6
29.2
26.9
72.2
73.8
41.6
52.2
46.1
20.9
101
79.9
113
51.6
12.0
45.9
2.76
48.9
7.79
df
40
31
54
65
57
62
72
61
64
56
71
66
59
54
50
60
67
76
51
40
57
54
62
68
111
Table S5C
R
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
112
pHT
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR AG
0.16
0.22
0.24
0.25
0.22
0.29
0.21
0.01
0.21
0.29
0.22
0.31
0.35
0.27
0.25
0.39
0.39
0.33
0.18
0.17
0.33
0.25
0.28
0.23
Intercept
38.2
21.3
8.97
-0.97
4.51
20.0
4.64
67.9
20.4
-15.6
6.40
-14.8
-34.5
-13.1
-3.51
-48.7
-45.3
-30.6
20.8
18.2
-30.7
-12.2
-17.4
-0.51
p-value
5.1E-02
4.0E-02
1.1E-04
1.9E-06
9.1E-06
2.4E-04
2.2E-03
8.5E-01
4.2E-07
9.8E-07
2.6E-09
2.9E-09
3.0E-10
7.9E-10
3.3E-05
2.2E-08
5.3E-12
1.6E-10
6.2E-04
5.2E-03
3.8E-10
1.7E-06
5.7E-06
1.4E-05
R2
0.09
0.13
0.24
0.29
0.29
0.20
0.12
0.00
0.33
0.35
0.39
0.42
0.49
0.51
0.29
0.41
0.51
0.42
0.21
0.18
0.50
0.34
0.28
0.24
F-value
4.03
4.60
17.5
27.3
23.8
15.2
10.1
0.03
31.7
30.2
46.3
47.0
57.2
55.8
20.9
41.6
69.6
54.6
13.3
8.73
57.1
28.7
24.6
21.8
df
41
31
55
66
57
61
73
61
64
56
71
66
59
53
50
60
68
76
51
41
57
55
62
69
113
Table S5D
R
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
114
pHT
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR PDG
0.61
1.04
0.54
0.12
-0.19
0.93
0.39
-0.36
0.49
0.51
0.48
0.58
0.60
-0.15
-0.13
0.60
1.02
0.87
0.56
-0.58
1.30
-0.12
1.05
0.12
Intercept
76.0
-30.0
114
288
432
46.1
164
407
185
162
162
141
127
419
383
130
-39.0
43.2
152
598
-178
382
-43.5
287
p-value
2.2E-01
0.8E-01
0.5E-01
5.2E-01
3.2E-01
3.0E-03
1.4E-01
4.8E-01
2.3E-03
1.1E-01
6.4E-05
4.3E-03
2.8E-01
5.8E-01
4.9E-01
0.7E-01
1.9E-04
8.7E-03
1.2E-01
5.4E-01
1.5E-03
7.1E-01
1.1E-03
6.1E-01
R2
0.13
0.68
0.26
0.02
0.11
0.40
0.10
0.03
0.31
0.14
0.41
0.26
0.06
0.02
0.03
0.17
0.40
0.22
0.14
0.04
0.44
0.01
0.44
0.01
F-value
1.71
6.49
4.57
0.44
1.09
11.8
2.42
0.52
11.4
2.91
21.3
9.65
1.22
0.32
0.49
3.76
18.5
7.96
2.62
0.41
14.0
0.14
14.7
0.26
df
11
3
13
21
9
18
21
17
26
18
31
28
18
18
15
18
28
28
16
10
18
14
19
31
115
Table S6
R
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
116
pHT
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR SV
13718
2138
11707
8059
7255
7624
5766
2173
7115
7057
5610
5006
5170
5305
5321
6785
5423
4650
6916
6655
7752
5501
6881
7458
Intercept
-3215616
-139761
-2833339
-1712831
-1535912
-1494633
-1220624
-134507
-1522373
-1590537
-1078650
-924638
-996768
-1016548
-849248
-1469956
-1140896
-759855
-1563000
-1361934
-1883126
-1112094
-1588100
-1821479
p-value
2.0E-06
2.8E-01
2.1E-10
5.5E-16
1.3E-15
2.0E-12
3.3E-08
9.4E-03
8.3E-10
2.4E-13
1.6E-12
1.5E-13
2.2E-16
2.7E-10
3.6E-07
1.6E-12
3.9E-13
2.4E-08
1.5E-07
4.2E-08
5.1E-11
2.3E-10
8.6E-16
5.4E-11
R2
0.60
0.05
0.55
0.64
0.69
0.56
0.34
0.11
0.50
0.63
0.57
0.59
0.69
0.55
0.41
0.58
0.56
0.33
0.49
0.51
0.58
0.53
0.68
0.50
F-value
37.9
1.24
62.4
116
123
77.1
38.3
7.21
54.8
93.1
78.8
89.3
129
61.3
34.6
80.2
82.7
38.7
39.7
44.6
69.4
60.5
121
62.8
df
25
22
51
64
55
61
73
59
55
54
60
61
58
51
49
58
64
77
42
42
50
54
58
62
117
118
Table S7
R
pHT
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.78
7.74
7.83
7.99
7.99
7.99
Respiration
rates
0.54
0.63
0.91
0.31
0.55
0.81
0.27
0.61
0.74
0.83
0.53
0.47
0.25
0.60
0.19
0.50
0.26
0.24
Intercept
p-value
R2
F-value
df
-101
-120
-204
-61.0
-108
-181
-42.8
-134
-187
-221
-118
-108
-48.2
-151
-4.42
-118
-45.3
-39.7
6.4E-04
4.2E-05
9.5E-04
1.2E-03
4.8E-04
2.3E-04
0.8E-01
4.9E-02
9.3E-06
2.6E-02
2.0E-04
6.6E-05
1.9E-02
1.4E-04
1.4E-01
6.5E-03
1.7E-02
2.3E-02
0.53
0.83
0.91
0.57
0.62
0.58
0.19
0.27
0.72
0.29
0.59
0.69
0.30
0.61
0.13
0.38
0.45
0.42
17.9
47.7
48.3
17.1
21.3
22.3
3.57
4.72
40.5
6.09
23.0
31.3
6.78
24.8
2.46
9.80
8.24
7.17
16
10
5
13
13
16
15
13
16
15
16
14
16
16
16
16
10
10
119
120
Table S8
R
pHT
6.5a
6.5b
6.5c
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
6.44
6.50
6.45
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
Mortality
rates
8.56
7.77
8.60
3.67
4.16
4.75
4.17
4.20
0.67
3.07
4.55
4.07
3.37
1.79
4.79
3.19
4.23
4.35
3.96
-0.25
2.23
4.81
5.02
4.24
4.16
4.31
4.11
Intercept
p-value
R2
F-value
df
-93.4
-111
-94.1
-80.4
-85.8
-106
-108
-102
-71.0
-96.2
-96.2
-95.7
-99.6
-90.3
-116
-109
-105
-95.4
-104
-52.2
-92.4
-108
-105
-95.7
-88.3
-99.3
-95.2
6.5E-05
1.5E-04
1.5E-03
2.9E-09
1.4E-08
4.6E-09
1.1E-09
2.2E-07
2.7E-01
2.4E-06
5.2E-07
4. 8E-08
8.3E-06
7.9E-05
1.5E-08
1.2E-05
4.0E-08
2.2E-07
2.6E-08
5.2E-01
7.1E-05
4.7E-09
2.8E-07
1.1E-07
1.5E-07
8.1E-08
3.8E-08
0.84
0.81
0.69
0.85
0.82
0.84
0.87
0.77
0.06
0.70
0.74
0.80
0.66
0.57
0.82
0.64
0.80
0.76
0.81
0.02
0.57
0.84
0.76
0.78
0.77
0.79
0.80
48.7
38.8
20.4
108
88.3
102
122
61.8
1.30
44.0
54.8
75.6
36.4
25.1
87.4
34.4
77.4
61.6
81.9
0.42
25.5
102
59.8
67.9
65.0
70.5
77.9
9
9
9
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
19
121
Table S9A
R
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
122
pHT
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR POL
11.0
15.2
19.3
21.2
24.2
13.5
2.93
10.3
26.7
32.6
11.1
13.2
38.1
32.0
37.0
35.0
13.3
14.2
48.4
49.6
49.3
37.9
47.2
20.0
Intercept
153
109
183
196
152
234
332
292
192
131
348
331
117
179
131
178
344
333
58.8
80.8
76.7
174
124
349
p-value
5.8E-05
1.7E-05
4.1E-17
1.0E-18
8.4E-17
2.8E-08
3.4E-02
4.8E-07
9.0E-28
9.5E-21
2.5E-06
1.3E-08
9.7E-18
6.8E-18
3.1E-16
3.8E-24
2.3E-08
6.7E-08
1.1E-23
1.5E-23
1.7E-33
2.4E-20
1.5E-29
3.1E-14
R2
0.29
0.40
0.71
0.69
0.71
0.39
0.06
0.34
0.85
0.79
0.27
0.39
0.72
0.75
0.74
0.82
0.37
0.32
0.86
0.92
0.92
0.79
0.87
0.57
F-value
19.5
24.4
142
149
137
40.2
4.65
31.7
354
213
26.2
42.2
148
162
142
277
40.0
35.8
321
442
688
208
429
91.0
df
47
37
57
67
57
63
73
61
64
56
71
66
59
54
50
60
68
76
51
41
57
55
62
69
123
Table S9B
R
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
124
pHT
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR AL
1.69
6.18
9.24
7.39
10.7
7.22
-1.92
3.86
14.6
17.2
4.21
4.16
19.7
13.0
21.8
19.4
7.22
9.91
27.8
26.1
25.0
21.9
25.3
12.5
Intercept
21.7
-44.9
-27.2
0.46
-31.1
-23.9
65.8
25.3
-34.8
-64.3
57.6
66.7
-73.2
-24.1
-83.5
-57.5
45.5
15.5
-126
-94.0
-91.7
-45.0
-72.7
30.8
p-value
1.5E-01
6.5E-05
5.9E-19
3.7E-14
2.6E-18
3.4E-08
8.9E-03
4.9E-04
2.7E-25
2.7E-25
2.5E-03
2.9E-03
6.7E-17
3.4E-14
4.0E-20
2.9E-23
5.5E-06
4.2E-11
3.3E-26
2.7E-18
4.8E-24
4.2E-14
2.3E-30
3.9E-18
R2
0.05
0.42
0.75
0.58
0.74
0.40
0.09
0.18
0.82
0.86
0.12
0.13
0.70
0.66
0.82
0.81
0.26
0.44
0.89
0.85
0.84
0.65
0.88
0.67
F-value
2.11
21.5
174
92.3
162
40.1
7.22
13.6
286
335
9.87
9.54
135
104
224
255
24.4
59.2
417
226
291
102
460
138
df
37
30
57
66
57
60
72
61
64
56
70
66
59
54
50
60
68
76
51
41
57
55
62
69
125
Table S9C
R
7.1a
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
126
pHT
6.98
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR PDL
6.79
9.19
7.01
12.4
6.98
3.55
6.35
22.2
26.8
5.05
7.05
35.9
20.2
21.1
25.5
9.21
11.6
51.3
42.4
38.7
39.6
36.3
11.1
Intercept
-42.3
-32.2
16.8
-24.3
41.6
39.4
-13.2
-161
-229
74.0
59.0
-332
-130
-110
-173
68.9
-12.8
-492
-369
-306
-346
-295
9.36
p-value
3.1E-02
3.1E-03
2.3E-03
4.7E-04
1.4E-02
7.5E-02
1.8E-04
1.3E-08
2.1E-03
4.6E-02
6.8E-02
2.8E-04
2.6E-02
1.3E-01
1.4E-02
3.9E-04
9.5E-06
3.1E-08
2.8E-05
7.6E-08
7.8E-08
2.8E-09
7.0E-08
R2
0.36
0.50
0.38
0.76
0.29
0.14
0.57
0.70
0.42
0.12
0.11
0.53
0.25
0.15
0.29
0.37
0.41
0.70
0.68
0.65
0.71
0.71
0.51
F-value
6.10
13.2
12.2
28.5
7.32
3.49
22.8
64.2
12.9
4.32
3.61
20.2
5.91
2.55
7.40
16.3
26.1
60.3
33.4
52.0
57.7
71.0
43.0
df
11
13
20
9
18
22
17
27
18
31
28
18
18
15
18
28
38
26
16
28
24
29
41
127
Table S9D
R
7.3a
7.3b
7.3c
7.5b
7.5c
7.7b
7.7c
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
128
pHT
7.25
7.19
7.24
7.34
7.44
7.64
7.67
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR PRL
2.25
1.36
4.81
4.66
10.7
3.58
1.08
4.57
5.13
6.95
8.76
8.99
-7.60
4.82
4.50
9.72
7.03
Intercept
-20.3
-0.52
-51.2
-58.5
-135
-31.6
4.10
-39.6
-51.2
-77.2
-105
-99.1
124
-42.3
-37.7
-106
-73.7
p-value
2.8E-02
1.6E-01
9.9E-03
2.0E-01
4.8E-07
2.1E-01
3.5E-01
1.0E-01
0.9E-01
1.3E-07
5.4E-06
1.1E-03
1.6E-01
0.4E-01
1.1E-01
6.7E-06
9.3E-08
R2
0.32
0.23
0.39
0.37
0.78
0.14
0.06
0.18
0.19
0.69
0.57
0.55
0.19
0.22
0.17
0.69
0.61
F-value
6.11
2.38
8.90
2.32
61.5
1.77
0.95
3.04
3.31
54.6
33.1
16.9
2.30
4.71
2.93
39.2
47.9
df
13
8
14
4
17
11
14
14
14
24
25
14
10
17
14
18
31
129
Table S10A
R
6.5a
6.5b
6.5c
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
130
pHT
6.44
6.50
6.45
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR BW
-1.75
2.63
-1.89
0.73
-1.78
6.10
7.75
7.77
5.98
5.46
4.49
7.76
7.64
6.90
5.04
7.73
7.76
6.60
7.55
5.67
6.26
8.14
6.35
6.75
4.44
7.19
4.48
Intercept
149.63
144.60
156.49
171.81
188.94
165.09
165.84
158.94
173.69
171.39
176.89
163.67
165.81
170.08
182.03
167.16
167.06
177.12
169.35
185.95
175.30
169.39
176.73
177.45
182.95
175.22
186.92
p-value
2.1E-01
2.3E-02
0.8E-01
1.5E-01
1.1E-02
5.9E-20
3.1E-31
3.8E-20
5.7E-20
3.3E-30
3.5E-15
1.2E-34
1.5E-24
2.7E-27
3.9E-19
7.6E-25
5.7E-29
2.8E-16
6.7E-27
5.6E-24
3.5E-30
7.3E-22
8.1E-15
1.5E-18
3.4E-08
2.6E-20
4.5E-17
R2
0.04
0.10
0.07
0.02
0.07
0.61
0.75
0.62
0.55
0.72
0.51
0.79
0.71
0.68
0.57
0.70
0.77
0.57
0.72
0.65
0.71
0.68
0.57
0.59
0.30
0.61
0.51
F-value
1.60
5.52
3.29
2.14
6.77
139
294
143
129
262
90.6
369
206
221
126
205
284
106
236
181
258
174
96.0
125
36.9
141
104
df
43
48
44
92
84
90
98
88
104
104
88
96
86
102
96
89
86
80
90
98
106
81
71
87
85
92
99
131
Table S10B
R
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
132
pHT
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR POG
10.9
24.8
8.60
10.6
12.3
10.3
3.38
7.37
13.0
22.0
8.97
7.67
21.0
18.2
16.5
23.6
8.86
11.9
19.6
8.96
18.1
7.78
18.1
2.01
Intercept
47.2
-101
151
121
96.6
164
174
139
130
63.9
169
212
83.4
87.6
86.7
55.4
189
157
96.8
181
104
178
116
260
p-value
2.2E-03
2.4E-04
5.3E-05
1.2E-07
2.4E-07
2.9E-06
6.1E-04
5.6E-09
2.4E-10
7.3E-17
8.0E-08
2.6E-05
2.0E-10
4.8E-14
4.6E-08
3.2E-15
5.9E-08
8.6E-11
1.7E-13
2.7E-03
2.1E-09
2.0E-03
9.9E-12
2.1E-01
R2
0.21
0.36
0.26
0.35
0.38
0.30
0.15
0.43
0.47
0.71
0.34
0.24
0.50
0.65
0.45
0.65
0.36
0.43
0.66
0.20
0.47
0.16
0.53
0.02
F-value
10.7
17.2
19.3
35.3
34.4
26.5
12.9
45.9
56.4
140
35.8
20.5
58.7
102
41.4
110
37.3
56.8
98.5
10.3
50.5
10.6
69.7
1.64
df
40
31
54
65
57
62
72
61
64
56
71
66
59
54
50
60
67
76
51
40
57
54
62
68
133
TableS10C
R
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
134
pHT
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR AG
1.58
0.54
3.63
2.96
2.48
1.50
-0.27
0.75
2.99
3.99
1.92
2.34
4.49
4.36
4.50
5.15
3.31
2.71
3.86
3.33
5.08
4.16
4.11
1.18
Intercept
58.58
71.80
44.39
51.70
51.11
94.52
76.92
62.37
59.28
47.38
65.69
68.05
45.87
38.83
41.72
41.07
57.39
58.31
49.54
49.69
41.82
40.67
46.94
73.82
p-value
1.2E-01
8.3E-01
4.0E-09
3.3E-07
1.3E-04
4.7E-02
5.7E-01
3.4E-02
1.6E-10
6.9E-09
4.5E-05
1.2E-06
2.2E-08
1.5E-12
7.9E-07
1.2E-08
3.5E-13
3.3E-10
1.4E-05
7.4E-04
2.1E-10
3.0E-10
3.6E-07
1.3E-02
R2
0.06
0.01
0.47
0.33
0.23
0.06
0.01
0.07
0.48
0.45
0.21
0.30
0.41
0.61
0.39
0.42
0.54
0.41
0.31
0.25
0.51
0.52
0.34
0.09
F-value
2.47
0.045
48.8
32.3
16.9
4.12
0.320
4.71
57.9
46.5
18.9
28.5
41.8
84.5
31.8
43.6
80.8
52.2
23.1
13.3
59.4
58.9
32.4
6.52
df
41
31
55
66
57
61
73
61
64
56
71
66
59
53
50
60
68
76
51
41
57
55
62
69
135
TableS10D
R
7.1a
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
136
pHT
6.98
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR PDG
7.67
5.42
-0.65
-4.46
7.01
2.85
8.39
7.34
28.6
4.47
-1.23
36.4
27.7
-1.29
18.5
1.18
4.20
12.3
-61.5
32.6
4.85
30.3
-3.60
Intercept
130
227
347
430
265
251
144
265
-42.4
283
388
-146
-30.8
349
115
357
323
230
1157
-60.5
266
-22.4
400
p-value
0.7E-01
0.8E-01
8.1E-01
0.4E-01
0.1E-01
2.5E-01
3.1E-03
4.5E-03
5.2E-04
0.2E-01
5.7E-01
4.4E-03
1.5E-04
8.8E-01
0.7E-01
5.8E-01
1.0E-01
3.2E-01
3.7E-04
2.5E-03
6.4E-01
2.6E-03
0.4E-01
R2
0.26
0.22
0.00
0.39
0.31
0.06
0.41
0.27
0.50
0.17
0.01
0.37
0.56
0.00
0.17
0.01
0.10
0.06
0.73
0.41
0.02
0.39
0.13
F-value
3.92
3.67
0.06
5.68
7.95
1.43
11.9
9.70
17.8
6.22
0.34
10.6
22.9
0.02
3.72
0.31
2.94
1.06
27.7
12.3
0.23
12.0
4.48
df
11
13
21
9
18
21
17
26
18
31
28
18
18
15
18
28
28
16
10
18
14
19
31
137
Table S11
R
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.7e
7.7f
7.7g
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
8.1e
8.1f
8.1g
138
pHT
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.62
7.59
7.71
7.78
7.74
7.83
7.99
7.99
7.99
7.99
8.00
8.00
GR SV
89837
54194
113094
87305
87726
51760
19514
16178
83966
77133
31563
28442
50028
83667
76107
89457
33796
46623
103521
125884
110323
68693
87254
53609
Intercept
-455613
-131846
-293590
58548
-28885
335846
476319
399106
52602
121601
557371
530171
342393
39821
272761
103749
447883
404856
-30385
-100209
-65837
236901
124453
348393
p-value
3.4E-04
3.3E-03
1.5E-15
7.9E-20
1.5E-14
4.0E-07
1.3E-02
1.8E-02
7.4E-14
4.9E-13
1.6E-04
2.6E-05
3.4E-07
1.5E-15
4.6E-09
7.8E-16
6.1E-08
4.0E-11
1.7E-08
1.2E-12
2.2E-11
1.7E-10
1.1E-17
4.4E-08
R2
0.41
0.33
0.72
0.73
0.66
0.35
0.08
0.09
0.64
0.62
0.21
0.25
0.36
0.72
0.51
0.68
0.37
0.43
0.53
0.70
0.60
0.53
0.72
0.39
F-value
17.2
10.9
129
173
108
32.3
6.42
5.95
98.5
89.2
16.3
20.8
33.2
129
50.4
121
37.6
59.2
48.2
99.4
73.5
61.6
149
38.9
df
25
22
51
64
55
61
73
59
55
54
60
61
58
51
49
58
64
77
42
42
50
54
58
62
139
140
Table S12
R
pHT
7.1a
7.1b
7.1c
7.3a
7.3b
7.3c
7.5a
7.5b
7.5c
7.7a
7.7b
7.7c
7.9a
7.9b
7.9c
8.1a
8.1b
8.1c
6.98
7.01
7.11
7.25
7.19
7.24
7.42
7.34
7.44
7.34
7.64
7.67
7.78
7.74
7.83
7.99
7.99
7.99
Respiration
rates
2.32
2.30
7.59
2.82
5.52
6.26
2.53
6.08
7.19
8.39
4.48
4.33
2.02
5.75
2.22
5.02
3.12
3.14
Intercept
p-value
R2
F-value
df
10.1
9.89
-9.50
11.6
11.3
1.77
15.9
1.58
-9.76
-30.0
13.5
10.2
18.4
-0.90
38.2
12.4
17.5
17.0
1.2E-04
5.9E-05
1.4E-05
9.6E-04
6.2E-05
4.2E-05
1.1E-02
9.8E-03
1.2E-08
3.3E-03
3.9E-05
7.0E-06
3.2E-02
5.7E-07
2.7E-02
3.3E-03
3.9E-03
4.5E-03
0.62
0.81
0.98
0.58
0.72
0.66
0.36
0.41
0.88
0.45
0.66
0.77
0.26
0.80
0.27
0.43
0.58
0.57
25.6
43.9
282
18.0
33.6
31.1
8.35
9.15
112
12.2
31.6
48.1
5.51
63.8
5.90
12.0
14.0
13.3
16
10
5
13
13
16
15
13
16
15
16
14
16
16
16
16
10
10