Sunflower (Helianthus annuus) Fatty Acid Synthase complex: Enoyl-[acyl
carrier protein]-reductase genes.
Planta
Irene González-Thuillier1, Mónica Venegas-Calerón1*, Rafael Garcés1, Penny von WettsteinKnowles2 and Enrique Martínez-Force1
1
Instituto de la Grasa (CSIC), Av. Padre García Tejero, 4, 41012 Sevilla, Spain.
2
Biology Department, Copenhagen University, Ole Maaloees Vej 5, 2200-Copenhagen N,
Denmark.
Corresponding author:
Mónica Venegas-Calerón
Email: mvc@ig.csic.es
Supplementary Figure S1
Supplementary Fig. S1 Phylogenetic comparison of plant and algae enoyl-[acp]-reductase
homologues of the E. coli FabI protein. H. annuus sequences are shown in bold
Supplementary Figure S2
A
1
2
kDa
150
kDa
135
100
95
72
75
50
52
42
34
26
37
25
20
17
15
10
T S AP
T S AP
HaENR1
B
KDa
150
100
75
50
37
25
20
15
10
T S AP T S AP
XL1-Bl
JP1111
HaENR2
Supplementary Fig. S2 Purification of H. annus recombinant ENR proteins. (A) Coomassie
blue-stained SDS-PAGE (left) and Western blot (right) with anti-His antibodies of
recombinant HaENR1 from XL1-Bl grown at 37 oC. Arrow points to expected position of
HaENR1.(B) Coomassie blue-stained SDS-PAGE of recombinant HaENR2 from wild type
XL1-Bl and the mutant strain JP1111 (fabIts) grown at 37 and 32 oC, respectively. T, total E.
coli extract; S, soluble fraction; AF, affinity purified using one passage through a Ni2+charged HisTrap FF column; kDa, size markers