OIE Reference Laboratory Reports
Activities in 2012
Name of disease (or topic) for which you
are a designated OIE Reference
Laboratory:
Red sea bream iridoviral disease
Address of laboratory
National Research Institute of Aquaculture,Fisheries
Research Agency, 422-1 Nakatsuhamaura, Minamiise, Mie 516-0193, JAPAN
Tel.:
(+81)599 66 1830
Fax:
(+81)599 66 1962
e-mail address:
RSIV -lab@fra.affrc.go.jp
website:
http://nria.fra.affrc.go.jp/e/DTC.html
Name (including Title) of Head of
Laboratory (Responsible Official):
Division Director
Name (including Title and Position) of OIE
Reference Expert:
Director for Research
Date of submission to the OIE
24 January 2013
Dr. Norihsia Oseko
Dr. Kazuhiro Nakajima
Instructions
This form should be used by an OIE Reference Laboratory to report activities that took place from January
through December of the past year (2012), unless otherwise stated, and must be submitted by the end of
January every year.
Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE
should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference
Laboratories, available at:
http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/
Each ToR (blue italicised text) has been placed as a heading covering the group of questions related to it.
Please note the red italicised text is given as guidance and should be deleted from your report and substitute
with your data. Examples are based on past Annual Reports or have been invented.
The questionnaire represents a means of gathering information on activities carried out by OIE Reference
Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.
This annual report will remain available for consultation on the OIE web site:
(http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/):
Annual reports of OIE Reference Centres, 2012
1
OIE RL for « Red sea bream iridoviral disease » – « Dr. Kazuhiro Nakajima » – « Japan »
Total number of test performed last year
Test recommended by the OIE
2.
Indirect diagnostic tests
Nationally
Internationally
IFAT
155 tests
0 tests
Direct diagnostic tests
Nationally
Internationally
Cell Culture (GF cells)
30 tests
0 tests
PCR gene (1-F and 1-R primer sets)
10 tests
0 tests
Did your laboratory produce or store imported standard reference reagents officially recognised by
the OIE or other international bodies?
 Yes
3.
No
Did your laboratory supply standard reference reagents to OIE Member Countries?
 Yes
2
No
Name of recipient
OIE Member
Countries and of
institutions
Type of
reagent
available
Related
diagnostic
test
Produced/
stored
Amount supplied
nationally
(ml, mg)
Amount supplied
internationally
(ml, mg)
Monoclonal
antibody (M10)
IFAT
Produced
5 ml
-
Shizuoka prefectural
research institute of
fishery
Monoclonal
antibody (M10)
IFAT
Produced
5 ml
-
Mie prefecture
fisheries research
institute
Monoclonal
antibody (M10)
IFAT
Produced
10 ml
-
Wakayama prefecture
fisheries research
institute
Monoclonal
antibody (M10)
IFAT
Produced
5 ml
-
Tottori prefectural
fisheries
experimentally
station
Monoclonal
antibody (M10)
IFAT
Produced
5 ml
-
Oita prefectural
agriculture, forestry
and fisheries research
center
Monoclonal
antibody (M10)
IFAT
Produced
5 ml
-
Tokushima
agriculture, forestry
and fisheries
technology support
center
Monoclonal
antibody (M10)
IFAT
Produced
10 ml
-
Kochi prefecture
fisheries research
institute
Positive control
DNA for PCR
PCR
Produced
-
l
Dr. Jorge Vasquez
Torres, Universidad
Austral de Chile
Positive control
DNA for PCR
PCR
Produced
-
l
Dr. Nimal Jayaweera,
Central Veterinary
Annual reports of OIE Reference Centres, 2012
OIE RL for « Red sea bream iridoviral disease » – « Dr. Kazuhiro Nakajima » – « Japan »
Type of
reagent
available
Related
diagnostic
test
Produced/
stored
Amount supplied
nationally
(ml, mg)
Amount supplied
internationally
(ml, mg)
Name of recipient
OIE Member
Countries and of
institutions
Investigation Centre,
Sri Lanka
Positive control
DNA for PCR
PCR
Produced
-
l
Universiti Putra
Malaysia, Prof.
Mohamed Shariff
Positive control
DNA for PCR
PCR
Produced
-
l
Tai Lung Veterinary
Laboratory, AFCD, Dr.
Chow Cheuk Hung,
Hong Kong SAR
Positive control
DNA for PCR
PCR
Produced
l
-
Tottori prefectural
fisheries
experimentally
station
Positive control
DNA for PCR
PCR
Produced
l
-
Wakayama prefecture
fisheries research
institute
4.
Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference
reagents?
 Yes
5.
No
Did your laboratory produce vaccines?
No
Yes
6.
Did your laboratory supply vaccines to OIE Member Countries?
 No
Yes
7.
Did your laboratory develop new diagnostic methods validated according to OIE Standards for the
designated pathogen or disease?
 Yes
8.
No
Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen
or disease?
 No
Yes
Name of the new test or diagnostic
method or vaccine developed
Description and References (Publication, website, etc.)
qPCR
Abstruct of "Annual meeting of The Japanese Society of Fish Pathology
9.
Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes
10.
 No
Did your laboratory provide expert advice in technical consultancies on the request of an OIE
Member Country?
 Yes
Annual reports of OIE Reference Laboratories, 2012
No
3
OIE RL for « Red sea bream iridoviral disease » – « Dr. Kazuhiro Nakajima » – « Japan »
Name of the OIE Member Country
receiving a technical consultancy
Purpose
How the advice was
provided
USDA APHIS Veterinary Services, USA
Confirmation of PCR method
Remote assistance
11.
Did your laboratory participate in international scientific studies in collaboration with OIE Member
Countries other than the own?
 No
Yes
12.
Did your Laboratory collect epizootiological data relevant to international disease control?
 No
Yes
13.
Did your laboratory disseminate epizootiological data that had been processed and analysed?
 No
Yes
14.
15.
What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a)
Articles published in peer-reviewed journals: ................. 1
b)
International conferences: .............................................. -
c)
National conferences: ...................................................... 1
d)
Other: ............................................................................... -
Did your laboratory provide scientific and technical training to laboratory personnel from other OIE
Member Countries?
 No
Yes
16.
a)
Technical visits: ................................................................
b)
Seminars: .........................................................................
c)
Hands-on training courses: ..............................................
d)
Internships (>1 month): ...................................................
Does your laboratory have a Quality Management System certified according to an International
Standard?
Yes
17.
 No
Is your laboratory accredited by an international accreditation body?
Yes
 No
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease
concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or
Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
 Yes
19.
Did your laboratory organise scientific meetings on behalf of the OIE?
Yes
4
No
 No
Annual reports of OIE Reference Centres, 2012
OIE RL for « Red sea bream iridoviral disease » – « Dr. Kazuhiro Nakajima » – « Japan »
20.
Did your laboratory participate in scientific meetings on behalf of the OIE?
 No
Yes
21.
Did your laboratory exchange information with other OIE Reference Laboratories designated for the
same pathogen or disease?
 Yes
22.
No
Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated
for the same pathogen or disease by organising or participating in proficiency tests?
Yes
No
Purpose of the proficiency tests:
(validation of a diagnostic protocol:
specify the test; quality control of
vaccines: specify the vaccine type, etc.)
Role of your Reference
Laboratory (organiser/
participant)
No.
participants
EU proficiency tests 2012
participant
20<
23.
Participating OIE
Ref. Labs/
organising OIE Ref.
Lab.
OIE RL for VHS,
Denmark
Did your laboratory collaborate with other OIE Reference Laboratories for the same disease
on scientific research projects for the diagnosis or control of the pathogen of interest?
 Yes
No
Title of the project or contact
Scope
Name(s) of relevant OIE Reference
Laboratories
Assessment of virulence determinant
of viral haemorrhagic septicaemia
virus (VHSV) towards trout
Assessment of virulence determinant
of viral haemorrhagic septicaemia
virus (VHSV) towards trout
OIE RL for VHS, Denmark
24.
Did your laboratory organise or participate in inter-laboratory proficiency tests with
laboratories other than OIE Reference Laboratories for the same disease?
Yes
25.
 No
Did your laboratory place expert consultants at the disposal of the OIE?
Yes
 No
List of publications for questionnaire of 14.
Scientific publications in peer-reviewed journals
J. Kurita and K. Nakajima; Review Megalocytiviruses. In:Viruses 4 (2012) 521-538.
Poster presentations
T. Ito, Y. Yoshiura and K. Nakajima; Dynamics of titer and quantity of viral genome of red sea bream
iridovirus (RSIV) under the different water temperature, Annual meeting of The Japanese Society of Fish
Pathology, 9 September 2012, Shimonoseki, Japan.
Annual reports of OIE Reference Laboratories, 2012
5