DNA Barcoding for Identification of Species of Tabanus (Diptera: Tabanidae) in Eskişehir A. Yavuz KILIC1, Sumeyra N. SANAL DEMIRCI2 1 Anadolu University, Faculty of Science, Department of Biology,Eskisehir, TURKEY 2 Anadolu University, Graduate School of Sciences Department of Biology, TURKEY [email protected] DNA barcoding lately have been become as an alternative method to assist in the identification of species. A DNA barcoding is a short gene sequence taken from standardized region of the genome, used to identify speices. The fly family Tabanidae (horse flies) includes an estimated 4500 extant species distributed throughout the world. The biology and taxonomy of horse flies has an important historical legacy but modern phylogenetics-based revisionary work has lagged behind other dipteran groups due to a generational reduction in the number of horse fly taxonomists and to a perceived ‘difficult’morphology which is often homogeneous for many standard dipteran characters and highly adapted in others. The literature on the Tabanidae fauna of Turkey, it was found that 171 species and 15 subspecies have been reported. In this study, Tabanus bifarius, Tabanus bromius, Tabanus glaucopis were analyzed. PCR was carried out to amplify by using COI gene region. Phylogenetic analyses were conducted using the Neighbor-Joining method in MEGA5. The bootstrap consensus tree inferred from 1000 replicates is taken to represent the evolutionary history of the taxa analyzed. This study we showed that use of DNA sequences in a systematic could be useful for preventing misidentifications among species.