University of Puerto Rico
Río Piedras Campus
College of Natural Sciences
Nutrition and Dietetics Program
Program: Bachelor of Science in
Nutrition and Dietetics
Human Biochemistry Laboratory, NUTR
4159
First Semester, 2012-2013 Academic Year
Sec. 0U1, Wednesday, 1:00 p.m. – 3:50 p.m.
Room NEP 577
Michelle Schelske Santos, Ph.D.; mschelskeuprrp@gmail.com, mschelske@uprrp.edu; ext. 5798, 2792
Date
Assignment
22Aug12
Academic Recess – Tropical Storm Isaac
29Aug12
LAB: Syllabus, Preparation of LAB Notebooks
LAB Excel workshop – Data Tables and Graphs;
Register for course in Edu 2.0
General Laboratory Safety; Equipment assignment
Intro to instrumentation; (bring lab coats and lab
notebooks)
Quiz in Edu2.0; open 24h before lab
LAB Safety (Biohazard)
Post-conference written quiz; practical lab quiz
Micropipette and Pipette Techniques; practical lab
quiz
LAB – DNA Extraction
LAB – Tissue Homogenation of Protein Samples
Quiz in Edu 2.0; open 24h before lab
LAB Computer Workshop – Info Databases and
your Search Strategy (Biblioteca Ciencias
Naturales; Prof. Pura Centeno)
Assign.: Search Strategies, DNA & Protein
Methods
LAB – Total Protein Determination
Quiz in Edu 2.0; open 24h before lab
Due: Assignment Search Strategies DNA &
Protein Method
LAB Expt: Active Acidity and pH;
Quiz in Edu2.0 open 24h before lab
LAB: Protein Electrophoresis of blood fractions I
Quiz in Edu 2.0; open 24h before lab
05Sep12
12Sep12
19Sep12
26Sep12
03Oct12
10Oct12
17Oct12
Topic
Introduction to course & prep of Lab Notebook
Introduction to Excel for Lab Reports--Water
content of foods
General Lab Safety; Introduction to Scientific
Instrumentation
Blood-borne Pathogens and Biohazard
Disposal (OPASO; Dr. Warner Ithier)
Micropipette and Pipetting Techniques
DNA Extraction from strawberries
Homogenation of animal & vegetable protein
Biochemical methods for DNA and protein
analyses in the field of nutrition
Protein content of animal versus vegetable
protein on microscale using microplate reader;
Comparison of protein content of human milk,
cow milk and infant formula
Active Acidity and pH
Preparation of blood fractions
Preparation for and dry run of electrophoresis
Dra. M. Schelske Santos; NUTR 4159
24Oct12
LAB: Protein Electrophoresis of blood fractions II
Quiz in Edu 2.0; open 24h before lab
LAB OP1 – (50 pts.; 1h)
Bioinformatics Assignment
Academic Recess - Elections
Protein electrophoresis, gel staining & drying
Colorimetric Determination of Vitamin C: Raw
vs. Processed Foods
28Nov12
LAB: Expt. 30, Vitamin C
Quiz in Edu 2.0; open 24h before lab
Due Bioinformatics (Edu 2.0)
LAB – Expt. Membrane Structure and Function;
Quiz in Blackboard; open 24h before lab
Due SDS-PAGE Lab (in notebook)
Extraction of DNA from whole blood (TBconfirmed)
05Dec12
Electrophoresis of DNA
12Dec12
LAB – Lipids and Fiber
Quiz in Edu 2.0; open 24h before lab
Lab OP 2 – (50 pts.; 1h)
Due: Lab Notebooks
LAB: Glycolytic Fermentation
Horizontal agarose gel electrophoresis of
nucleic acids
Dietary Lipids, Fiber & Fattaché
31Oct12
07Nov12
14Nov12
21Nov12
(Rep.22aug12)
TBA, Dec.
17-21, 2012
XXXXXX
XXXXXX
Experiments to date, including protein electro.
Use of experimental and stored data
Blood Typing
Digital Microscopy of Blood Cells
Extraction of DNA from student leucocytes
(Experiments from second half of semester)
Anaerobic fermentation of sugars by yeast
Anaerobic fermentation of glucose by muscle
Oral Presentations & Discussion of assigned lab Includes all previous lab experiences and
procedures, results, and conclusions or related related topics
topics
Due: Written assignment of topic
This schedule is subject to change based on unforeseen events, natural disasters, scheduling of events, and
changes in laboratory equipment and material arrival dates. In the event of any change, a new schedule will be
developed and available in Edu 2.0.
Edited by MSS, 02oct12
sem. 1, 2012-2013
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