RAMU KAKUMANU
Chemist/ Toxicologist
rakakuma@utmb.edu
Eastern New Mexico University, Portales, NM May-2010
M.S. Chemistry, Biochemistry Emphasis
GPA: 3.61/4.00
Acharya Nagarjuna University, Andhra Pradesh, India April 2006
B.S. Pharmaceutical sciences
GPA: 3.87/ 4.00
 Chemical analysis by Agilent 5975 Gas Chromatography/Mass Spectroscopy, Thermo scientific LTQXL UHPLC/MS, UV-Vis spectroscopy, NMR spectroscopy, IR spectroscopy,
Organic synthesis.
 Protein purification and characterization, Ion exchange chromatography, SDS-PAGE, Gel electrophoresis, Enzyme kinetics, Polymerase chain reaction (PCR).
 Responsibilities include chromatographic analysis of biological and environmental samples for organic contaminants, metabolites and Biomarker candidates using GC/MS and LC/MS using chemstation , Xcalibur software’s.
Plans, coordinates and directs moderately complex research experiments following general technical procedures.
Research Projects 2012 to present:
U19 grant funded by NIH/NIEHS “Gulf Coast Health Alliance: Health Risks Related to the
Macondo Spill (GC-HARMS)”, which involves PETROGENIC POLYCYCLIC AROMATIC
HYDROCARBONs (PAHs) ANALYSES OF MARINE SPECIES AFTER THE DEEP WATER
HORIZEN OIL SPILL. Performed Extraction of the PAH’s was performed using the

QUACHERs/dsPE extraction method and make sure samples ready for analysis using a Gas chromatography-Mass spectrometry
 “Dissecting the mechanisms of Alcoholic liver disease" Funded by John Sealy Memorial
Endowment Fund for Biomedical Research
My role in this project was to measure Blood Alcohol Levels in Rat Blood samples by Head space Gas Chromatography, FID, measuring Fatty acid Ethyl esters which are the biomarkers of Liver disease caused by alcohol abuse, using GC-FID, GC/MS instruments and report results to Supervisor.
 “Hepatic Steatosis and Lipid Metabolome" funded by NIH R01, AA016364, NIH, National
Institute on Alcohol Abuse and Alcoholism, My role in this project is to perform TLC on mouse tissue, and measure Fatty acid ethyl esters by GC-FID, GC/MS.
 Effects of BisphenolA(BPA) on ASTHMA PATHOGENESIS, This project focuses on the important topic of a potential new environmental contributor to the epidemic of childhood asthma.My role in this project was to develop analytical method to detect BPA in blood samples of infants, young children, I worked on method develoment using GC/MS
 Establishes and oversees quality control measures for assigned project operations.
Makes modification to research procedures as determined by Principal
Investigator. Providing guidance to junior research staff.
 Responsibilities include the forensic analysis of blood and other body fluids for the presence of drugs and other toxic substances using Gas chromatography/Mass spectrometry.
Preparing legally defensible reports from GC/MS, providing testimony in court of law when needed.
 Received appropriate training to perform analysis on competency or proficiency tests and performed toxicology analysis on case work for DWI, DRE, DFSA & OMI cases to identify drugs of abuse which utilizes standard established procedures.
 Analysis of Controlled substances in Blood and Urine samples which includes Opiates,
Cocaine, Morphine, Heroin, Keto Opiates which includes Hydrocodone, Oxycodone,

Cannabinoids (THC) Using GC/MS, Drug analysis of Biological fluids for the presence of toxins. Solid phase extraction of drugs in Biological fluids, report toxicology results in
Laboratory Information systems (LIMS).
 Provided testimony in Magistrate, Municipal courts in New Mexico which involves explaining toxicology results to attorneys, jury.
Research Assistant Jan-2011-May2011
University of Alaska Fairbanks, Dept. of Plant pathology
Provided assistance with research on biological control of plant diseases, performed aseptic culturing techniques, spectrophotometer, electrophoresis, fluorescent microscope, Ion exchange Chromatography, data analysis, collection and entry
Jan- 2008 to May-2010
Graduate Teaching Assistant/ Research Assistant
 Prepared lab experiments, monitored labs, proctored and graded exams, assisted freshmen, junior and senior level students for organic chemistry, biochemistry, general chemistry labs, monitoring inventory of chemicals and reagents, waste management, MSDS inventory.
 As a part of master’s thesis worked on a research project which includes operation of fermentor, French press and performed various electrophoretic techniques, bacterial cell culture techniques, bio analytical characterization of membrane bound cytochromes.
Research Associate July 2006 to june 2007
 Involved in the project entitled “Identification of novel drug target for influenza A virus
(molecular modeling and computer-aided drug design)”.
 Thesis work in master of science program includes Isolation, purification and characterization of cytochrome c-549 and oxygen consumption studies of bacteria Halothiobacillus nealpolitanus.
 Studies on oxygen consumption in the obligate chemolithoautotroph Halothiobacillus nealpolitanus Presented at New Mexico Bioinformatics Symposium (NMBIS), March-
2009, Santa Fe, NM.Isolation, purification and characterization of cytochrome c-549 from
Halothiobacillus nealpolitanus Presented at 35th annual Student Research conference at
Eastern New Mexico University, NM.

 "Production of gentamicin by microbial strain Micromonospora echinospora" as partial fulfillment of Undergraduate requirement
 Recipient of graduate school research grant ENMU award
 Outstanding paper presentation award 35th student research conference ENMU
 Graduate student Senator, ASENMU
 Member of ENMU Graduate Student Association
 Merit scholarship award K.L.P public school, Guntur, India
Dr.Bhupendra Singh Kaphalia, Ph.D.
Professor, Department of Pathology;
Director, Analytical Chemistry CoreUniversity of Texas Medical Branch
301 University Boulevard
Galveston, TX 77555-0438.
Dr.Ghulam Ahmad Shakeel Ansari, Ph.D.
Professor, Department of Biochemistry & Molecular Biology;
Professor, Department of Pathology;
Co-Director, Integrative Health Science Facility Core, National Institute for Environmental Health
& Sciences Center; Member, Sealy Center for Environmental Health and MedicineThe
University of Texas Medical Branch at Galveston
301 Univesity Boulevard
Galveston, TXÂ 77555-0438, Office: (409) 772-3107, Fax: (409) 772-9679, Office: (409) 772-
3655
Fax: (409) 747-1763 sansari@utmb.edu
,
Dr. Rong-Jen Hwang, PhD
.
(505) 383-9086