Supplementary Figure S1 Legend.
RNA protection assay. Two micrograms of total RNA extracted from leaves of each
genotype was hybridized by long and short probes indicated in the map. To distinguish
the 5´ ends of ndhD and the 3´ ends of psaC, two RNA probes with identical 5´ ends but
different 3´ ends were used. Protected fragments indicated by x are present in the
negative control without Arabidopsis RNA and a result of possible secondary structures
in probe RNAs. The bands labeled by c and d correspond to the protected fragments of
the 5´ ends of ndhD and the 3´ ends of psaC, respectively. The bands labelled by c and d
correspond to the fully protected fragment by dicistronic precursor RNA. RNA ends
were roughly assigned on the map based on the information in tobacco24. Long probe is
672 bp, the short probe 616 bp.
Supplementary Figure 1.
RNA protection assay. Two micrograms of total RNA
extracted from leaves of each genotype was hybridized by long and short probes
indicated in the map. To distinguish the 5' ends of <I>ndhD</I> and the 3' ends of
<I>psaC</I>, two RNA probes with identical 5' ends but different 3' ends were used.
Protected fragments indicated by x are present in the negative control without
<I>Arabidopsis</I> RNA and a result of possible secondary structures in probe RNAs.
The bands labeled by c and d correspond to the protected fragments of the 5' ends of
<I>ndhD</I> and the 3' ends of <I>psaC</I>, respectively. The bands labeled by c+d
correspond to the fully protected fragment by dicistronic precursor RNA. RNA ends
were roughly assigned on the map based on the information in tobacco ²⁴.