DATA SHEET NO
THE UNIVERSITY OF BIRMINGHAM
School of Biological Sciences
Name of
Supervisor
Assessment Number
Dr. J.R.Green
Supervisor's
Signature
Note
Date of Assessment
1837
30/7/1
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment (USP/90/CHRA/16).
Guidance is also available from Notes on Completing the Chemical Hazard and Risk Assessment Form.
A Word for Windows document template for this form is available from the School Safety Officer.
When completed, a copy of this form must be lodged with The School Safety Officer Mr P G Dearn
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
S104
List names where possible
All undergraduate project students, all postgraduate students, all postdoctoral assistants, all supervisors in S104
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
trifluoroacetic acid (used in protein extraction)
Attach copies of data sheet(s)
NAME
trifluoroacetic acid
(TFA)
5
HAZARD
corrosive
harmful
RISK PHRASES
DATASHEETNO
Corrosive. Causes severe burns.
may be harmful if absorbed through skin, if
inhaled, if swallowed
Harmful to aquatic organisms, may cause
long-term effects in the aquatic environment.
INTENDED USE
Give brief details and attach protocol/instructions
protein extraction from filamentous fungi. used neat in a fume hood to extract spore/mycelial surface proteins.
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate
causes burns.
may be harmful if absorbed through the skin.
may be harmful if inhaled.
material is extremely destructive to the tissue of the mucous membranes and upper respiratory tract, eyes and
skin.
inhalation may result in spasm, inflammation, and edema of the larynx and bronchi, chemical pneumonitis and
pulmonary edema.
symptoms of exposure may include burning sensation, coughing, wheezing, laryngitis, shortness of breath,
headache nausea and vomiting.
emits harmful combustion or decmposition products: carbon monoxide, carbon dioxide, hydrogen fluoride.
harmful to aquatic animals – may cause long-term adverse effects in the aquatic environment.
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
acceptable as small quantities are used in protected environment.
use protective clothing.
use good chemical practice.
8
CONTROL MEASURES
Additional to Good Chemical Practice. Specify type or performance of control measure
wear suitable protective clothing, gloves and eye/face protection.
use only in a chemical fume hood.
safety shower and eye bath.
wash contaminated clothing before reuse.
discard contaminated shoes.
wash thoroughly after handling.
do not breathe vapour.
do not get in eyes, on skin, on clothing.
avoid prolonged or repeated exposure.
compatible chemical-resistant gloves.
chemical safety goggles.
faceshield.
keep tightly closed in a well-ventilated place.
store in a cool dry place.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
none
10
MONITORING
Performance of control measures,
none
Personal exposure
11
Health Surveillance
WASTE DISPOSAL PROCEDURE
avoid release to the environment.
harmful to aquatic organisms – keep residue etc. in bottle which will be properly disposed of (via stores).
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
30/7/6
13
EMERGENCY ACTION AND CONTACTS WHERE NECESSARY
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
in case of fire, use noncombustible extinguishing media. emits toxic fumes under fire conditions.
accidental release measures: wear self-contained breathing apparatus, rubber boots and heavy rubber gloves.
cover with dry lime or soda ash, pick up, keep in a closed container and hold for waste disposal. ventilate area
and wash spill site after material pickup is complete.
CONTACT
Jon Green
PHONE Ex. 5574
Evacuation, protection for personnel involved in clean-up, Special First Aid
TO PROTECT PERSONNEL
if swallowed, wash out mouth with water provided person is conscious. call a physician immediately. if inhaled,
remove to fresh air. if not breathing, give artificial respiration. if breathing is difficult, give oxygen. in case of skin
contact, flush with copious amounts of water for atleast 15 minutes. remove contaminated clothing and shoes. call
a physician. contamination of the eyes should be treated by immediate and prolonged irrigation with copious
amounts of water. assure adequate flushing of the eyes by separating the eyelids with fingers.
firefighting: wear self-contained breathing apparatus and protective clothing to prevent contact wth skin and eyes.
CONTACT
Jon Green
PHONE Ex. 5574
Clean-up/decontamination
TO RENDER SITE OF EMERGENCY SAFE
contact a licensed professional waste disposal service to dispose of this material. dissolve or mix the material with
a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber.
CONTACT
Jon Green
PHONE Ex. 5574