Supplementary Table 1 Pathological observations of neoplasm tumor in nude bearing melanoma model after injection of five
types of cells (showed by HE staining)
Microscopic observation
Presence
Group
of
Visual observation
Cell arrangement
Cell morphology
Nuclear morphology
complicated
necrosis
Small black spots that Normal epidermal
Brown
bulge
out
from
the cells, and
pigment
with
granular
granular
HEM
skin,
dark
Inside
brown
surface of nude mice and
or
with No nuclear atypia
distinct pigment
different sizes and
boundary
with
the distributed outside
shapes
surrounding tissues
the cells
None
tissue
Tumors bulge out from
Cancer
the surface of nude mice Cancer
cells
cells unequal
with
sizes,
Degeneration
of
Large nucleus, nuclear
skin,
appearing
brown,
with
red appear
nest-like abundant cytoplasm,
A375-WT
some
atypia,
indistinct and
alveolar appearing
and
cells
many
like
complicated
pathologic mitosis
boundary
with
the arrangement
polygons
surrounding tissues
Tumors
slightly
nude
mice
irregular necrosis
spindles
bulge
out from the surface of Cancer
A375-G6PDΔ
and
skin, appear
Cancer
cells
with
equal
sizes,
no
cells
nest-like
Few nuclear atypia
melanin
No tissue necrosis
granules
appearing nodular shape, arrangement
found
with distinct boundary
A375-G6PDΔ-
Tumors bulge out from Cancer
cells Cancer
cells
with Nuclear atypia and
No tissue necrosis
G6PDWT
the surface of nude mice appear
nest-like unequal
sizes, pathologic mitosis
by
skin, appearing gray red, arrangement
appearing
with partial adhesion to
polygons
like
the surrounding tissues
Tumors bulge out from
the surface of nude mice
Cancer
Cancer
A375-G6PDΔ-
Nuclear atypia and
unequal
common pathologic No tissue necrosis
appearing
arrangement
difficulty to be moved
sizes,
nest-like
partial adhesion to the
surrounding tissues and
with
cells
skin, appearing red, with
appear
G6PDG487A
cells
like
mitosis
polygons
Supplementary figure legends
Fig.1 HE staining of tumor tissues produced by injection of 5 types of cells
Fig.2 Immunohistochemical staining of cyclin E protein in tumors formed by
injection of 4 types of cells
Fig. 3 Immunohistochemical staining of p53 protein in tumors produced by
injection of 4 types of cells
Fig. 4 Immunohistochemical staining of S100A4 protein in tumors produced by
injection of 4 types of cells
Fig. 5 Immunohistochemical staining of Fas protein in tumors produced by
injection of 4 types of cells
Fig. 6 Immunohistochemical staining of Bcl-2 protein in tumors produced by
injection of 4 types of cells
Fig. 7 Immunohistochemical staining of Bcl-xL protein in tumors produced by
injection of 4 types of cells