ORIGINAL RESEARCH Technical quality of root canal treatment of posterior teeth after rotary or manual preparation by university students. Ibrahim Abu-Tahuna, Mohammad A. Al-Rabab’aha, Mohammad Hammada, Ameen Khraisatb a Assistant Professor Department of Conservative Dentistry and Fixed Prosthodontics, Faculty of Dentistry, The University of Jordan, Amman, Jordan. b Professor, Department of Conservative Dentistry and Fixed Prosthodontics, Faculty of Dentistry, The University of Jordan, Amman, Jordan. Type of Article: In-vivo study Running Short Title: Satisfaction and Technical Quality of RCT Corresponding author’s address Dr Ibrahim Abu-Tahun Department of Conservative Dentistry and Fixed Prosthodontics The University of Jordan, Queen Rania Street, 11942 Amman, Jordan. Tel: (+962) 79 5607189 e-mail: ihtahun@yahoo.com 1 Abstract The Aim of this study is to investigate the technical quality of root canal treatment (RCT) reached by the undergraduate students as their first experience in molar endodontics using nickel-titanium (NiTi) files in a crown down approach compared to stainless steel (SS) standard technique. This study was carried out by the 5th year undergraduate students attending peer review sessions as a part of their training program , using two different questionnaires to assess the overall technical quality and potential problems regarding endodontic complications after root canal preparation with these two systems.The overall results indicated statistically significant difference in the performance of the two instrument systems in difficult cases showing better performance of the NiTi system and mean rotary preparation time (P < 0.001). Under the conditions of this study, novice dental students, using NiTi ProTaper rotary files, were able to prepare root canals, faster with more preparation accuracy , compared to canals of same teeth prepared with hand instruments. Key words: NiTi files , SS files , mishaps . 2 Introduction A plethora of investigations on manual and automated root canal preparation has been published over recent decades. Experimental and clinical evidence from these studies suggests that the use of Ni-Ti instruments results in improved preparation quality, accuracy and lower incidence of post-operative pain and discomfort (1). Recent prospective studies even with untrained operators and inexperienced undergraduate dental students demonstrated that improved , technical quality of root canal treatments obtained with NiTi instruments leads to better obturation results compared to stainless steel K-files ( 2, 3, 4), and advances in using NiTi technology and tapered files are seeing more universities adopt and incorporate rotary instrumentation as part of the endodontic curriculum ( 5, 6). Despite the results in vitro, randomized, clinical trials are required to evaluate the performance outcomes when using NiTi and SS instruments. Only one study (7) evaluated the performance and the incidence of complications of RCT performed by undergraduate dental students in Jordan using SS instruments and step-back technique. The objective of this study was to investigate, in vivo, the influence of instrument type for canal enlargement on the results obtained after using rotary and manual instrumentation techniques to assess the efficacy of the instrument as well as the technique and its clinical suitability. Assessment of students’ performance, opinions and views regarding the use of NiTi and SS files will provide insights into their technical clinical abilities and knowledge, and valuable feedback on teaching effectiveness. Materials and Methods 3 Two questionnaires, comprising multiple-choice questions , one dealing with rotary nickeltitanium preparation results , while the other questionnaire dealing with stainless steel (SS) preparation were first developed. The final series of questions covered demographics, issues related to the technical results after using both systems, patterns of training in NiTi use , as well as students’ opinions on their satisfaction and which system to integrate in their future practices. Each questionnaire consisting of 2 parts was delivered to the undergraduates of the 5th grade of the Faculty of Dentistry, The University of Jordan at the 1st visit. The 1st part of the questionnaire was answered by the undergraduates during the various stages of treatment, and the 2nd part completed and signed by the senior supervisor at the end of treatment, and personally returned. A letter explaining the objectives of the survey to the students was attached to every questionnaire. A pilot testing with a random sample of 20 undergraduate students was conducted by filling out the two questionnaires and evaluated protocols modified accordingly into a final revision to assure reliability and credibility of the results. Staff members involved in data collection were instructed on the nature of the study to ensure adequate use of data and avoid misleading results and conclusions. Cross - assessment to check and confirm the results was completed directly either by the supervisors or staff members undertaking the research. Data collection and statistical analysis The data collection period extended from December 2010 to May 2011. Questionnaires were numerically coded to ease the procedure and each response was allocated its own column. The study was performed by analyzing the responses to the 2 questionnaires. The data collected from the responses was entered manually into a Microsoft Excel spreadsheet (Microsoft Corporation 4 WA,USA) that was imported into SPSS TM ( SPSS Inc Chicago , IL,USA) statistical software version 17.0 , presented as tables for comparison of proportion and submitted to percentage analysis. Statistical software analysis was carried out using the Chi-square test to determine statistically significant differences between the technical quality of root fillings and instrument system in different groups of teeth , and in relation to the degree of difficulty of the case. Tooth Selection Inclusion and Exclusion Criteria One hundred eighty nine teeth included in this study , comprising maxillary and mandibular molars requiring primary non-surgical endodontic treatment were selected according to the criteria used by the undergraduate student clinic, Department of Conservative Dentistry, The University of Jordan. Wisdom teeth were excluded. All RCTs were carried out in the clinic session by 5th year undergraduate students under the supervision of senior staff members with an average staff to student ratio of 1: 9 . The " inexperienced " dental students were asked to shape two molar teeth, one molar with a sequence of manual SS files using conventional step back technique and the other molar was prepared with rotary Ni-Ti files in a crown-down manner. Instruments and Instrumentation Techniques Access cavity preparations were done. Apical patency was determined by inserting an ISO # 08 # 10 file and the working lengths were determined using working radiographs and / or apex locator (Root ZX ‘J. Morita USA. Inc.). Irrigation was not a parameter in this study. Selected teeth were divided into 2 groups and prepared with either NiTi files or SS K-files. 5 Group 1: Hand file instrumentation technique The endodontic treatments performed in the hand technique, comprising 327 root canals were prepared by conventional SS K-Flexofile hand files (Dentsply-Maillefer, Ballaigues, Switzerland) and Gates Glidden burs (Dentsply-Maillefer, Ballaigues, Switzerland) , using the traditional serial step-back preparation technique (8). To achieve adequate preparation of the root canal system, in routine cases, a minimum apical preparation size 35 has been suggested for mesial canals and # 45 in palatal and distal canals in the SS group. Gates Glidden drills number 2-4 were used in the cervical part of the canal. In difficult cases a minimum apical size of no. 30 for mesial canals has been suggested (9). Group 2: Rotary instrumentation technique Prior to instrumentation, the 5th year students involved in our study were given a 2 days workshop including a hands on training course to the use of rotary instrumentation with ProTaper files. After establishing of a smooth glide path using SS KFs #15 or 20, the canals were prepared using ProTaper rotary instruments (Dentsply-Maillefer, Ballaigues, Switzerland), connected to an x smart low torque motor (Dentsply). A special kit of ProTaper, NiTi rotary endodontic shaping files, each working pack contains six files (S1,S2,F1,F2,F3&SX) featuring a variable taper was selected and distributed to each student. All canals were prepared using S1, S2, F1, and F2. The SX and F3 ProTaper files are occasional files according to the manufacturer recommendations. Manufacturer recommendations and instrument sequence should be taken into account for the actual speed used. Root canals of all groups were obturated with gutta-percha points and AH26 sealer using a cold lateral condensation technique. 6 Evaluation Criteria Every canal needs to be evaluated on its own merits. For the evaluation of the postoperative quality of root canal preparation , a detailed list of safety issues were reported and analyzed including parameters- problems of special interest created by instruments and techniques. One of the purposes of this project was to observe and obtain sufficient data on the amount of changes in endodontic anatomy produced by hand and/or rotary instruments to draw definite conclusions on their usefulness. Pre-, intra- and post-obturation periapical radiographs including angulated radiographs of each tooth taken by the means of Endo-ray® were carried out for each treated tooth under standardized conditions, so that these radiographs, taken may be compared to quantify and measure root canal differences and deviations between pre- and post-preparationobturation root canal anatomy. By nalyzing the pre and post-obturation radiographs, senior supervisors evaluated every endodontic case performed by the same student. Because of a certain observer bias that may occur under the conditions of this study a double and triple check by 2 researchers ‘ staff members‘ was employed. To ensure reproducibility and allow a comparison of the results, canal dimensional were studied and evaluated under a 10- x magnifying lens and xray viewer. The technical quality of root canal shaping and obturation on films was examined based on traditional evaluating parameters for the assessment of root canal preparation according to criteria employed in other similar studies, aiming to reflect radiographic appearance of the obturation according to length of the “ white line“ in relation to the radiographic apex as well as density and taper (10, 11). The tooth was considered as a unit and the overall technical quality of root obturation was scored according to the grading system used in the Department of Conservative Dentistry, The University of Jordan , where : 7 (A), equals Excellent (B), equals Good (C), equals Average (D), equals Failed Total treatment “working " time for each endodontic case performed, recorded as " working visits ", was also calculated by the same student for each system. Results The total sample material used in this investigation consisted of the periapical radiographs of patients who had received root canal treatment at the Faculty of Dentistry, The University of Jordan. The descriptive statistic analysis was employed. Of the (220 ) questionnaires distributed, (31 ) were not completed or dropped as being incomplete, giving a total of ( 86 %) completion, expressing a high return index of the questionnaires (Table 1). A final total of 189 molars were treated . The high return index of the questionnaires (86%) expresses a positive acceptance of the undergraduate students of the advantages and impact of this study on their teaching discipline and also the intensive follow up of the study by the research team. All 189 distributed and answered questionnaires in this study were analyzed . Of the (189) treated teeth , 96 (50.8%) were treated by rotary NiTi instruments , and 93 ( 49.2 %) were treated by stainless steel hand files. The prevalence of tooth type and location receiving root canal treatment are shown in (Table 2). The overall root fillings graded as 'excellent' in this study was 51 (28.5%) (Table 2). More 8 specifically , root fillings graded as 'excellent' in difficult cases were 34 (40.1% ) all in the NiTi group as opposed to none (0) in the SS hand files group. When cases rated ' good ' were considered, the overall 'excellent plus good' frequency of root canal fillings out of the (83) difficult cases included in this study for ProTaper files and SS hand files was 57 (68.7%) and 7 (8.4%) respectively indicating statistically significant difference (P < 0.001) in the performance of the two instrument systems in difficult cases (Figure 1). The total number of cases graded as 'failed' in this study was 20 (10.5%). Of these failed cases 19 (95%) belong to the SS hand file group, while only 1 failed case (5%) was treated with NiTi files (Table 2). The overall better root preparation quality results produced by NiTi files is indicated clearly by ( table 2&3) showing an overall increased incidence of procedural mishaps and a higher failure rate of teeth treated with SS hand files compared to ProTaper files (P< 0.001). Ledge formation occupied the highest incidence 17 (37.7%) among all the procedural mishaps recorded in this study (Figure 2). The incidence of ledging for ProTaper files and SS hand files was 1 ( 5.9%) and 16 (94.1%) respectively indicating statistically significant difference between the two instrument systems (P = 0.001). Mesiobuccal, mesiolingual and distobuccal root canals in the maxillary and mandibular 1st molars shared the higher incidence 42 (93.3%) of overall procedural mishaps . These teeth were also the most frequently ledged teeth 16 (94.1%) compared to one ledge (5.9%) in the 2 nd molar teeth ( Table 3 ). 9 More transportation and more dentin formation were created with hand files sharing equal frequency and rate , eight for each mishap (4.2%) vs. (0) compared to the NiTi instrumentation that achieved more correct preparation length (p = 0. 004). The overall incidence of instrument fracture in the total sample was 6 (3.2%), of which one (16.7%) was a NiTi file and 5(83.3%) hand files (p=0.105). With three instrument fractures ( 50 %) in the apical third, two (33.3%) in the middle of the root, and one instrument failure (16.7%) in the coronal part, Table 3, shows a statistically significant difference between the type of two instrument systems and their location in the canal (p=0.31). The incidence of perforation in the total sample was 6 (3.2%) , all in the SS hand file treated teeth , significantly more frequently compared to NiTi rotary preparation (P = 0.014). Most perforations were reported in the apical third 4 (66.7% ) compared to two perforations (33.3% ) in the middle third and no perforation occurrence in the coronal third (p= 0.01). The mean time required for manual preparation (Table 4) was significantly longer than that required for rotary preparation (P < 0.001). Seventy two teeth in the rotary group (75 %) required only 1 session to complete obturation compared to 3 sessions or more in all 93 (100%) of the cases in the hand file group. Discussion The shift to a rotary technique led to superior performance and its benefits became firmly established for a number of reasons not seen with hand instrumentation (12,13). Various studies rated preparation enlargement with NiTi root canal instruments superior compared with the Conventional stepback techniques even when used by undergraduate students (14 ). 10 In the present study Protaper and K-files, used by novice dental students ,were compared to assess radiographically the technical quality of the root filling and the incidence of endodontic procedural adverse effects on the extent of overall technical quality. 189 molar teeth , a total of (659 ) root canals , comprised of maxillary and mandibular first and second molars were treated by these senior dental students. At the start of study, patients were subjected to a clinical and radiographic examination and when indicated cases were chosen for the study , the medical and dental history of the patient were taken. Good diagnostics including the exposure and analysis of adequate radiographs to carefully determine the degree of difficulty of the case regarding root anatomy, the existence, extent, and position of canal curvatures as it may potentially have an impact on treatment quality outcome were involved. The procedural tooth was anesthetized and isolated with rubber dam. A direct straight line of access was optimized with high speed burs and canals carefully explored with straight, small hand files. Mechanical procedures involved, enlarging the canal wall by instruments and mechanical flushing action of the irrigant solution. EDTA was used only as a paste during preparation. The overall technical quality Different epidemiological criteria have been used to categorize root fillings and evaluate postoperative root canal shape , using extracted teeth or simulated root canals in resin blocks and clinical assessment as well (15) resulting in discrepancy in quality scores of treated teeth due to the different scoring parameters. While the length and homogeneity were used by most studies as evaluating criteria of the root filling quality (7, 16), the taper of the root filling was included in some other studies ( 10, 11) according to the guidelines of the European Society of 11 Endodontology ( 17). To allow better following of canal profiles and assessment of the overall technical quality of root canal fillings, three criteria , the length , density (voids) and taper (width) were used in the present study. Amongst the 189 root treated teeth, all 34 (40.1%) root fillings graded as 'excellent' in difficult cases were in the NiTi group as opposed to none (0) in the SS hand files group. Using dense root fillings rated as “ good” , raised the overall 'excellent plus good' frequency of root canal fillings in the (83) difficult cases included in this study for ProTaper files and SS hand files to 57 (68.7%) and 7 (8.4%) respectively indicating statistical significance in the performance of these two systems (P < 005). In addition, the percentages of failed cases indicated clearly that SS hand instruments did not perform as well as NiTi rotary instruments, results that are supported by other studies (18). While Georgelin-Gurgel et al.( 19) found manual instrumentation safer than rotary instrumentation in the hands of inexperienced students arguing the need for the theoretical and practical coursework in endodontics teaching in dental schools, taken with the in vitro experiments, or the very few studies that have assessed their impact when used in primary root canal treatment , this study suggests that the use of Ni-Ti rotaries does lead to enhanced technical quality, particularly for clinicians with less expertise (20, 21 ). These findings are in agreement with the conclusions reached by the vast majority of several earlier studies on postpreparation technical quality and support the hypothesis that, more efficient preparation results virtually free of gross preparation errors can be obtained with NiTi instruments, even by untrained operators and inexperienced dental students (22 ). The higher incidence of overall excellent quality and failed cases in maxillary and mandibular 1st molars may be explained by the larger number of these teeth in the total sample size 156 12 (82.5%) . Mesiobuccal and distobuccal canals of maxillary molars as well as mesiobuccal canals in these teeth are usually narrow, with great apical curvatures predisposing to a higher fracture frequency (23). Safety aspects Evaluation of universal findings in hand prepared curved root canals, was one of the purposes of the present study to assess post-operative disruptive changes and distortion of canal morphology. The highest mishap occurrence recorded was ledge formation indicating statistically significant difference between the two instrument systems (P = 0.000), with Mesiobuccal, mesiolingual and distobuccal root canals being the most frequently ledged 39 (88.2%). Dentin mud formation and transportation were not detected in the NiTi group compared to significantly more apical dentin plugs and more transportation with hand files sharing equal frequency and rate, 8 (4.2%) for each. NiTi instrumentation achieved better preparation length accuracy and better performance and behavior in preparing curved canals (p= 0.0004), results that were uniformly confirmed by earlier reports (24 ). One of the most commonly encountered procedural errors, when dental students perform molar endodontics is straightening of curved canals (25). While changes in the canal shape differed significantly between the different instruments at all measuring points (24), the NiTi instrument seemed to be safer because of the reduced amount of transportation towards the danger areas (26). The studies described so far indicate that it’s unlikely to prepare a root canal system and prevent dentin debris build up at the very apical part of the canal produced as a side effect of canal wall preparation (27). Rotary NiTi systems vary in their debris removal efficiency, possibly because 13 of differing flute designs, but crown down techniques have generally been reported to produce less apically extruded debris than stepback preparation (28). Even though irrigation was not a parameter in this study and the potential clinical impact of these preparation effects remain questionable (29), the findings of this study and other available studies underline the limited efficiency of hand instruments in cleaning the apical part of the root canal and indicate the crucial role of sufficient irrigation for disinfection of the canal system and prevention of procedural mishaps (27). Instrument fracture is a multifactorial problem (27) but torsional and flexural instrument fractures during root canal preparation is the major concern , (30). A preliminary canal enlargement by the students, to secure a smooth glide path in the apical 1/3 was mandatory before introducing rotary files to reduce cyclic fatigue and allow the instrument to shape canals to length with little or no procedural errors (31). Despite the common belief among dental practitioners that NiTi rotary instruments have an increased frequency of clinical fracture or deformation compared to retained stainless steel hand files, (32), and despite that Spili et al. (33) , found rotary instruments fractured in a specialist practice to be slightly more frequently than stainless steel files, current results do not support this view . On the basis of these results, the respective rate of fracture frequency by the undergraduate students in their initial therapies was higher and more frequent in hand file cases. Although higher in the hand files group , the breakage rate obtained in the present study is close to similar other studies (23, 28 ). A review of literature reveals lower frequency 0.4%– 4.4% of retained fractured NiTi rotary instrument than that for SS hand files after clinical use (34). The low incidence of NiTi rotary instrument fracture in this study and other similar early studies 14 supports the continued use of these instruments in root canal treatment although their impact on clinical outcomes remains uncertain (35). Reported perforations in clinical treatment as well as in experimental studies (26), agree with the current findings reporting (3.2% ) incidence of root perforations in the total sample , all in the SS hand file group. Most perforations resulted in stripped over-prepared curved canals from straightening along their inner aspect with higher prevalence of perforations in the middle third of the root canal (p=0.014 ). A recent study to evaluate the ability of a group of third-year dental students without any endodontic clinical experience who received a brief structured training session to use the ProTaper Instruments in curved canals on human molar teeth concluded that rotary instruments performed adequately with inexperienced operators (36). However, for less experience clinicians, adequate clinical handling needs to be emphasized (37). Working time In the present study, time for treatment was recorded aiming to evaluate the total working time for each instrument and technique and draw conclusions on their efficacy and clinical suitability in the hands of inexperienced operators. Decisive in many cases, data on working time show large differences for identical instruments and techniques, due to methodological problems as well as to individual factors (26, 38) and should be interpreted with caution as it might not exactly mirror the clinical situation (22). Although the total working time calculated in this study does not represent the significantly shorter actual “ real “ working time by using the NiTi instruments, the mean time required for manual preparation was significantly longer than that required for rotary preparation time (P<0.001). The findings of this study indicate that prior acquired experience with a hand 15 preparation technique was not reflected in an improved working time and that ProTaper NiTi shaping instruments were superior in preparing curved canals more rapidly. While some comparative studies (28), have shown no difference in working times when compared rotary NiTi preparations with manual instrumentation, the majority of comparative studies present some evidence for shorter working times for rotary NiTi preparation when compared with manual instrumentation. CONCLUSIONS Under the conditions of this study, the quality of root canal fillings in posterior molar teeth performed by undergraduate dental students in the University of Jordan , using NiTi rotary files as their first experience was safer, faster and better. In light of the undergraduate students performance using NiTi instruments and techniques , systematic incorporation of these systems into the preclinical and clinical curriculum and education about newer techniques and instruments seems promising and advocated to improve the success with molar teeth. Acknowledgement This study was supported by grants from the Deanship of Academic Research, The University of Jordan . The authors express their sincere gratitude to all the undergraduate students (2010-2011) and assistant staff for completing the data collection forms meticulously. Special thanks also to Prof Faleh El Sawaeer for his valuable contribution in conducting the statistical analysis of the results. 16 References 1. 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Ex vivo study on root canal instrumentation of two rotary nickel-titanium systems in comparison to stainless steel hand instruments. Int Endod J 2009 ;42:22-33. 38. Rhodes SC, Hülsmann M, McNeal SF, Beck P, Eleazer PD. Comparison of root canal preparation using reciprocating Safesiders stainless steel and Vortex nickel-titanium instruments. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011;111:659-67. 21 Table 1. Number of questionnaires distributed and responded per system and Prevalence of tooth type receiving root canal treatment per system. System used Delivered Questionnaires System used Responded Questionnaires Type of Tooth Total U6 L6 U7 L7 Rotary NiTi files 110 (100%) Rotary NiTi files 96 (87 %) 33 44 8 11 96(50.8%) Manual files 110 (100%) Manual SS 93 (85 %) 31 48 1 13 93(49.2%) Total 220 (100%) Total 189 (86%) 64 92 9 24 189(86%) 22 Table 2 Quality outcomes of overall results System Quality of work Degree of Difficulty Easy Difficult Excellent Good Failed Excellent Good Failed Excellent Good Failed NiTi 43 47 1 9 24 0 34 23 7 Hand 8 32 19 8 25 6 0 7 13 23 24 Figure 1 Overall quality of teeth obturation per system 50 45 40 35 30 25 20 15 10 5 0 System NiTi System Hand Excellent Good Average Failed 25 Table 3 Distribution of procedural errors per system, tooth and canal location System NiTi Hand Ledge Transportation Dentine Mud Type of teeth U6 L6 U7 L7 1 0 0 16 8 8 7 3 5 9 4 3 1 0 0 0 1 0 0 0 1 1 2 2 3 2 0 1 separation Coronal Middle Apical Coronal Middle Apical 0 0 0 0 2 4 3 3 0 0 Perforation 26 Figure 2 The incidence of mishaps per instrument system 18 16 14 12 10 8 6 4 2 0 System NiTi System Hand 27 Table 4 Total working time per system Working time System NiTi Hand 1 session 72 0 2 sessions 24 0 3 sessions 0 29 More than 3 sessions 0 64 28