The relationship between PAI-1 Polymorphism with recurrent implantation failure (RIF)
E-Mail:allafan2@yahoo.com
Allafan S. 1,3 * ,Hasanzadeh Nazarabadi M. 2,3,Mojarrad M. 2,3,Khayatzadeh J. 1,Shahrokh Abadi Kh. 1,Musavifar N. 4,Jalali M. 4
1-Department of Biology, Sciences Faculty,Mashhad Branch,Islamic Azad University,Mashhad,Iran 2. Department of Medical Genetics,School of
Medicine,Mashhad University of Medical Sciences,Mashhad,Iran,, 3. Medical Genetics Research Center,School of Medicine,Mashhad University of
Medical Sciences,Mashhad,Iran, 4. Montaserieh Infertility Treatment Center,,Mashhad University of Medical Sciences,Mashhad,Iran
Objectives
Results
RIF is the most common cause of unsuccessful
The frequency of 4G/5G genotype in control group,
pregnancy after IVF. Among the various causes of
RIF, the role of maternal genetic factors is of great
significance. PAI-1 polymorphism is one of the
notable polymorphisms in this field. High levels of
plasminogen activator inhibitor PAI-1 can be
decreased fibrinolysis and increased thrombosis. A
polymorphism in the promoter region of genes
identified in this gene is (4G) allele that compared to
normal (5G) produce more protein and thus reduce
fibrinolysis and can be increased thrombosis.
total participants, RIF1 group and RIF2 was %75,
%87.5, %80 and %95, respectively. The frequency of
5G/5G genotype in control group, total participants
patient group RIF 1, and patient group RIF 2 was
orderly %25, %5, %5 and %5.
4G/4G genotype was observed in none of the control
groups and patient group RIF 1, But the observed
%15 of patient RIF 2.
95
87.5
Monasteries Research & Clinical Center for Infertility of
Mashhad University of Medical Sciences. It was carried out
on 80 infertile couples after IVF from 2006 to 2011. Based
on the new definitions of RIF, participants were divided into
three groups: 1. Control group: Forty participants whose
transferred embryos were successfully implanted. The
selection criterion was the observation of gestational sac in
ultrasonography twenty days after IVF 2. Patient group RIF
1: Those participants who received IVF two times and six
embryos. In this group, no sign of pregnancy or forming
gestational sac was found. 3. Patient group RIF 2: Those
participants who received IVF at least three times and ten
embryos. Again no sign of pregnancy and gestational sac
was found. After receiving 5cc blood containing EDTA from
participants, the process of DNA extraction was performed.
Genotype of PAI-1 gene was determined by using PCRARMS technique. Gained frequencies in different groups
were compared with each other using chi-square statistical
analysis.
)
Number unit
75
80
60
Methods
This descriptive-analytical study was conducted in
100
80
25
15
7.5 5
0
40
5
0 5
20
0
CONTROL
TOTAL RIF
4G5G
RIF2
5G5G
RIF1
4G4G
Fig-1 (Results frequency of genotype)
shape-1 (A thermal cycler for PCR)
shape-2(gel electrophoresis)
shape-3(IVF steps)
Group
Number
5G allele
frequency
4G allele
frequency
RIF1
20
0/53
0/47
RIF2
20
0/45
0/55
total RIF
40
0/49
0/51
control
40
0/63
0/37
Tabel-1(allele frequency)
Conclusions
According to our results, 4G/4G Polymorphism of
PAI-1, is seen just in the second group of the patient )
RIF 2), with the new definition of recurrent
implantation failure. So, with increasing number of IVF
failure role of genetic factors become more significant.
Suggestions
1-Genetic counseling does in infertility centers
before IVF.
2-Investigation of PAI-1 gene variant in a larger
population of patients and control subjects are
examined.
3-The study of polymorphisms of molecules
associated with gene PAL-1 function in implantation
as well as their receptor molecules on the surface
of the endometrium and trophoblast take places on
samples simultaneously
.