GMS provides
Sacace Biotechnologies Srl
“One
stop
solution”
“Complete qPCR Solutions”
Founded in 2001
Sacace Biotechnologies s.r.l., is an innovative Italian company, located
in Como, Lombardia
2001-2009: Caserta, Campania region
Now: Como, Lombardia region
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Our Mission
To supply the best products to the
international diagnostics and
pharmaceutical industry, as well as to
hospitals and laboratories through a
global network of distributors located
in more than 60 countries all over the
world.
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Sacace Worldwide
Functions
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Research & Development
Manufacturing
Logistics
Marketing
Certifications (ISO 9001:2008 13485:2012,
CE Mark - Directive 98/79 EC)
• International external quality control
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Our PCR Kits
 Hepatitis Viruses (Quantitative tests for HCV, HBV,
HDV, HGV, HAV detection)
 Human Papilloma Viruses (Screening, Typing,
Quantitative detection of Low & High Risk
genotypes)
 STD (Multiplex detection of Chlamydia, Neisseria,
Trichomonas, Mycoplasma, Treponema, Candida ….)
 Herpes Viruses (Quantitative Multiplex detection of
CMV, EBV, HHV6, HSV, VZV)
Our PCR Kits
 TORCH infections (Toxoplasma, Rubella, Parvovirus)
 Respiratory infections (Tuberculosis, Influenza,
hRSV, Bordetella, Pseudomonas, Legionella)
 Neurological infections (Enteroviruses,
N.meningitidis, H.influenza, Str.pneumoniae)
 Gastrointestinal infections (Shigella, Salmonella,
E.coli, Rotavirus, Astrovirus, Norovirus, H.pylori)
Our PCR Kits
 Single Nucleotide Polymorphisms (SNP):
• Cardio Trombophilia Panel (FII, FV, FVII, FXIII, FGB)
• Cardio Hypertension Panel (ADD, AGT, NOS....)
• Folates Methabolism Panel (MTHFR, MTR, MTRR)
• Haemochromatosis Panel (HFE 187, HFE193, HFE845)
• Warfarin Panel (CYP2C9, CYP4F2, VKORC1)
Our PCR Kits
 Oncologic diseases (Mbcr-ABL, BRCA)
 Genetically Modified Organisms (Soya, Corn,
Rice, Tomatoes)
 Veterinary kits (CPV, PPV, CSVF, BVDV, ASF,
Brucella, TGEV...)
 RNA/DNA extraction kits
Our PCR Kits
Our Real Time PCR kits can work on multiple instruments:
 Qiagen Rotorgene TM
 Bio-Rad iQ5TM
 Cepheid SmartCycler TM
 Applied Biosystems 7300/7500/StepOne TM
 Stratagene MX3005P TM
 Roche LightCycler 2.0 TM
 Bioer LineGene-K TM
 SaCycler-96 TM from Sacace Biotechnologies
 ...more to come
SaCycler™-96
SaCycler™-96 Features
 4 or 5 channels multiplexing for discrimination of up to
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
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five targets in a single reaction well
Strong flexibility thanks to the 96-well format suited for
standard PCR microplates, test tubes and strips
Possibility to use tubes with different heights with
regulation of the matrix block
Optimal signal/noise ratio and absence of crosstalk
ensured by the unique design of the optical track including
a separate light source for each channel and a matrix CCD
camera
Light emitting diodes (LED) as a light source with a lifetime
of about 100,000 hours that does not require maintenance
SaCycler™-96 Features
 Wide dynamic range of detection using multiple exposure
method, which leads the optimization of signal registration
conditions to a whole new level, greatly simplifying or even
eliminating the need for fluorescence settings
 Main applications are Real-Time quantitation, single
nucleotide polymorphisms (SNPs) genotyping, melting
curve and gene expression analysis
 Instrument comes with the most used Sacace protocols
already installed in the software minimizing possibility of
error during programming.
SaCycler™-96 Characteristics
 Thermal block format 96 test tubes of 0.2 ml (12 х 8)
 Test tube type 0.2-ml test tubes for PCR (individual, in strips, 8 pieces each or a holder 12 x 8)
 Range of thermal block temperature control 0 C…100°С
 Resolution of temperature setting 0.1°С
 Absolute accuracy of temperature maintenance ±0.2°С
 Uniformity of thermal block temperature ±0.15 °С
 Maximum heating rate of the thermal block within temperature range of 4…99 °С 3.5 °С /s
 Maximum cooling rate of the thermal block within temperature range of 99….55 °С 2.5°С /s
 “Hot cover” temperature 105°С 1°С
 Actuating device of the thermal block Peltier elements
 Excitation source Light-emitting diode (LED)
 Detector CCD (charge coupled device) -matrix
 Number of the fluorescence measurement channels 4 or 5
 Excitation/detection wave length 470/525, 532/570, 585/633, 633/670, 690/750
SaCycler™-96 Comparison
Thermal System
Average ramp rate (°C/s)
Temperature accuracy (°C)
Uniformity of thermal block temperature
(°C)
Range of temperature (°C)
Optical System
Excitation spectrum
Detector
Emission filters/channels
Compatible dyes
Format
Test tube type (ml)
Supported volumes (µl)
Weight (Kg)
ABI 7500
Peltier
1.6
±0.25
BioRad CFX96
Peltier
3.3
±0.2
Qiagen
RotorGene Q
air cooled
15-20
±0.25
SaCycler-96
Peltier
3.3
±0.2
±0.5
4-100
±0.4
0-100
±0.01
ambient-99
±0.15
0-100
Tungsten halogen
lamp
5 filters
CCD camera
5
FAM, JOE, VIC,
NED, TAMRA, Rox,
TexasRed, Cy3, Cy5
96-well plates
0.2
20-100
64
LED
LED
450-684 nm
365-680
Photodiodes
PMT
6
6
FAM, JOE, VIC,
NED, TAMRA,
FAM, JOE, VIC,
Rox, TexasRed, NED, TAMRA, Rox,
Cy3, Cy5
TexasRed, Cy3, Cy5
96-well plates
0.2
up to 50
21.4
rotor disk
0.1 - 0.2
10-50
14
LED
470-750
CCD camera
4 or 5
FAM, JOE, HEX,
R6G, ROX,
TexasRed, Cy5,
Cy5.5
96-well plates
0.2
10-100
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Sacace thermalcycling protocols
UROGENITAL + CMV + EBV + TORCH + HPV INFECTIONS
Chlamydia trachomatis Real-TM
Neisseria gonorrhoeae Real-TM
Treponema pallidum Real-TM
Mycoplasma genitalium Real-TM
Ureaplasma parvum, Ureaplasma urealyticum Real-TM
Mycoplasma hominis Real-TM
Gardnerella vaginalis Real-TM
HSV I,II Real-TM
CMV Real-TM Qual
CMV Real-TM Quant
EBV Real-TM Qual
EBV Real-TM Quant
Trichomonas vaginalis Real-TM
Candida albicans Real-TM
Chlamydia trachomatis / Ureaplasma spp. / Mycoplasma genitalium
Real-TM
Chlamydia trachomatis / Ureaplasma spp. / Mycoplasma hominis RealTM
Neisseria gonorrhoeae / Chlamydia trachomatis / Mycoplasma
genitalium Real-TM
HSV I,II Typing Real-TM
Ureaplasma parvum / Ureaplasma urealyticum Real-TM
Trichomonas vaginalis /Neisseria gonorrhoeae Real-TM
Chlamydia trachomatis / Ureaplasma spp. Real-TM
Chlamydia trachomatis / Mycoplasma genitalium Real-TM
Candida albicans / Candida glabrata / Candida krusei Real-TM
HSV / CMV Real-TM
Mycoplasma hominis / Gardnerella vaginalis Real-TM
Ureaplasma parvum / Ureaplasma urealyticum / Mycoplasma
Real-TM
Trichomonas vaginalis / Neisseria gonorrhoeae /Chlamydia
trachomatis Real-TM
Neisseria gonorrhoeae / Chlamydia trachomatis Real-TM
Pseudomonas aeruginos Real-TM
Pneumocystis jirovecii Real-TM
CMV/EBV/HHV6 Quant
HHV6 Real-TM Quant
Rubella Real-TM
Toxoplasma Real-TM
Parvovirus B19 Real-TM Quant
HPV High Risk Screening Real-TM Quant
HPV High Risk Typing Real-TM
HPV 6/11 Real-TM
HPV 16/18 Real-TM
38 different kits
Sacace thermalcycling protocols
NEUROLOGICAL
Poliovirus Real-TM
NHS Meningitidis (N. meningitidis, H. influenzae, Str.
pneumoniae) Real-TM
2 different kits
RESPIRATORY INFECTIONS
Influenza A/B Real-TM
Influenza A Real-TM
Influenza A H5N1 Real-TM
Influenza A H1N1 Real-TM
Influenza A H1N1/H3N2 Real-TM
Influenza A H5/H7/H9 Real-TM
hRSV Real-TM
7 different kits
INTESTINAL INFECTIONS
Norovirus/Astrovirus/Rotavirus Real-TM
Shigella/Salmonella/Campylobacter Real-TM
EHEC Real-TM
Helicobacter pylori Real-TM
4 different kits
MTB INFECTION
MTB Real-TM
MTB Diff Real-TM
2 different kits
Thanks for your attention