Starting materials
Crude extract
Fast Centrifugal Partition Chromatograph
(FCPC®)
KROMATON, France
Purified compound
Final products
Worldwide specialist in centrifuges and liquid / liquid extractors for the chemical and pharmaceutical
industries
55 employees
7 employees in engineering and design
Design, fabrication, commercial, after-sales
Pr Jean Hugues Renault
Methodology, process
Worldwide specialist in CPC and mass
transfer (ion exchange)
Luc Marchal
Process optimization: cell design, sample analyses
Industrialization / Scale-up
REVIEW OF CENTRIFUGAL PARTITIONING
CHROMATOGRAPHY
 Liquid / liquid partitioning chromatography:
o Two immiscible liquid phases
o Achieved by mixing at least two solvents or solutions
 Stationary phase maintained by centrifugal force (rotation)
 Mobile phase is pumped through the stationary phase
 Separation of the solutes is achieved as a function of the
partition coefficient KD between the mobile and stationary
phases.
CPC MECHANISM …
Inlet
Fcentrifuge
Fc
Seals
between the
discs
Outlet
Light phase
Heavy phase
Movie
Fc
Descending Mode
Fc
Ascending Mode
FCPC RANGE
 All stainless steel, corrosion
resistant
 Quiet operation
 Easy access to rotors
Interchangeable rotors with different
capacities
1000 mL
50 mL
200 mL
FCPC RANGE
Unique 25 ml capacity rotor
Reduced weight and footprint
Pmax= 80 bars (1176 psig)
Rotation 3000 rpm
Milligram scale samples in
minutes
Separation of 2 proteins: myoglobin and lysozyme
Absorbance UV 220 nm
250
Column volume
32 ml
Solvent system
ATPS
PEG 1000/K2HPO4
200
150
Descending mode
100
50
Injected volume
1 ml d’une solution
Myoglobine 4,3 g/L
Lyzozyme 8,3 g/L
Flow rate
4 ml/min
Rotation
2000 rpm
Pressure
27 bars (397 psig)
Stationary phase
retention
28 %
0
0
5
10
15
20
25
30
Temps (min)
Protein
KD
Vr (ml)
tr (min)
Myoglobin
1.2
33.8
8.4
Lyzozyme
5.8
75.0
18.7
APPLICATION EXAMPLES
nonpolar
System Z
Hept
MeOH
ACN
Simple Lipids
polar
ARIZONA RANGE
Hept
MeOH EtOAc
ACN MtBE
Sterols
Phospholipids
Glycolipids
Water
System A
EtOAc
MtBE
Water
Inorganic elements
Various organic acids
Alkaloids
AQUEOUS
SYSTEM
TERNARY SYSTEMS
EtOAc
MtBE
BuOH Water
Water
BuOH TFA/Water
AcOH/Water
Polyphenols Polysaccharides
Anthocyanins
Flavonids
Water
+
PEG
+
Buffer
Proteins
Peptides
a-amino acids
Starting materials
Crude extract
Purified Compound
Final products
Plus d’informations sur WWW.KROMATON.COM
KROMATON ROUSSELET
45, Avenue Rhin et Danube –
07100 Annonay - FRANCE
Tel. : +33 (0)475 692 211
Fax : +33(0)4 75 67 69 80
j.meucci@kromaton.com
Thank you for your attention…