William S. Klug
Michael R. Cummings
Charlotte A. Spencer
Concepts of Genetics
Eighth Edition
Chapter 21
Dissection of Gene Function:
Mutational Analysis in Model
Organisms
Geneticists Use Model
Organisms That Are
Genetically Tractable
Features of Genetic Model Organisms
Yeast as a Genetic Model Organism
Saccharomyces
cerevisiae
Figure 21-1
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Figure 21-2
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Drosophila as a Genetic Model
Organism
Figure 21-4
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Figure 21-5
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The Mouse as a Genetic Model
Organism
Geneticists Dissect Gene
Function Using Mutations
and Forward Genetics
Generating Mutants with Radiation,
Chemicals, and Transposon Insertion
Screening for Mutants
Figure 21-7
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Figure 21-8
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Selecting for Mutants
Defining the Genes
(how many?)
Figure 21-9
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Dissecting Genetic Networks: Epistasis and
Pathways
Figure 21-10
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Extending the Analysis: Suppressors
and Enhancers
Figure 21-11
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Extending the Analysis: Cloning the Genes
Extending the Analysis: Biochemical Functions
Geneticists Dissect Gene
Function Using Genomics
and Reverse Genetics
Genetic Analysis Beginning with a
Purified Protein
Figure 21-12
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Figure 19-21
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Figure 21-13
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Genetic Analysis Beginning with a
Mutant Model Organism
(functional complementation)
Genetic Analysis Beginning with the
Cloned Gene
Figure 21-14
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Figure 21-15
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Figure 21-16
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Geneticists Dissect Gene
Function Using Genomics
and Reverse Genetics
Genetic Analysis Using Gene Targeting
Technologies
Figure 21-17
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Figure 21-18
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Figure 21-18a
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Figure 21-18b
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Figure 21-19
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Geneticists Dissect Gene
Function Using Functional
Genomic and RNAi
Technologies
RNAi: Genetics without Mutations
Figure 21-20
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High-Throughput Functional Genomics
Techniques
Gene Expression Microarrays
Genome-Wide Mapping of
Protein–DNA Binding Sites
Figure 21-21
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Geneticists Advance Our
Understanding of Molecular
Processes by Undertaking
Genetic Research in Model
Organisms: Three Case
Studies
Yeast: Cell Cycle Genes
Drosophila: The Heidelberg Screens
The Mouse: A Model for ALS Gene
Therapy
Figure 21-22
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21.5 Geneticists Advance Our
Understanding of Molecular
Processes by Undertaking
Genetic Research in Model
Organisms: Three Case
Studies
21.5.2 Drosophila: The Heidelberg Screens
Figure 21-26
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21.5 Geneticists Advance Our
Understanding of Molecular
Processes by Undertaking
Genetic Research in Model
Organisms: Three Case
Studies
21.5.3 The Mouse: A Model for ALS Gene
Therapy
Figure 21-29
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