AP Lab 4A
Paper
Chromatography
AP Lab 4A
Paper
- technique
that separates molecules from
Chromatography
each other
Nonpolar pigments are carried along at
different rates, b/c they are not equally
soluble in the solvent.
paper
polar
solvent
nonpolar
Nonpolar pigments are also attracted to
different degrees to the fibers of the
chromatography paper
AP Lab 4A
Paper
Beta carotene
= no polar groups (most
Chromatography
non polar)
Xanthophyl =
2 polar groups
Chlorophyll a = 5 polar groups
Chlorophyll
b = 6 polar groups
(most
CHROMOTOGRAPHY
SOLVENT
is
polar)
NONPOLAR
AP Lab 4A
Paper
polar pigments
dissolve in polar
Chromatography
pigments.
nonpolar pigments dissolve in nonpolar
pigments.
LIKE DISSOLVES LIKE
AP Lab 4A
Paper
polar pigments
dissolve in polar
Chromatography
pigments.
nonpolar pigments dissolve in nonpolar
pigments.
CHROMOTOGRAPHY SOLVENT is
NONPOLAR
AP Lab 4A
Paper
Beta carotene
= no polar groups (non
Chromatography
polar)
Xanthophyl =
2 polar groups
Chlorophyll a = 5 polar groups
Chlorophyll b = 6 polar groups (most
CHROMOTOGRAPHY PAPER is POLAR
polar)
AP Lab 4A
Paper
Chromatography
As nonpolar solvent moves up
the chromatography paper,
pigments are dissolved to
differing degrees and move
along with it at differing
degrees.
AP Lab 4A
Paper
Chromatography
The more non-polar a pigment,
the more soluble it is in a
nonpolar solvent…
The higher it will travel
Chromo pix here
Ratio
Factor
(this is a constant value)
Ratio =
Factor
distance moved by pigment (mm)___
total distance moved by solvent (mm)
(this is a constant value)
Ratio= distance moved by pigment (mm)_
Factortotal distance moved by solvent (mm)
(this is a constant value)