A Single Illumina HiSeq 2000 Paired-End Lane
HP LaserJet P4015
Black & White Printer
Letter Size Paper
Paired-End Lane:
2 x 100 bp reads
90 x 106 Reads
18 x 109 bp
8 days
18 (50) GB
data
Sequencing Cost
Letter Size Paper:
1” margins
12 pt font size
Times New Roman
~2,500 character
(UPPERCASE)
11 inches
Illumina HiSeq 2000
Printer:
~50 papes/min
(single sided)
Cartridge:
~24,000 pages
8.5 inches
$3,000
How much paper is needed to print data from 1 paired-end lane of HiSeq?
1 case = 10 reams
1 ream = 500 pages
Or
18 x 109 bp / 2.5 x 103 = 7.2 x 106 pages
Or
7.2 x 106 / 500 = 14,400 reams
And
14,4000 / 10 = 1,440 cases (12” x 10” x 18”)
1 printer + 300 cartridges + 100 days
A pile of cases 12 ft x 12 ft x 12ft
Supply Cost:
1,440 cases @ $40/each + 300 cartridges @ $300/each =
$147,600
To keep up with sequencing time, you will need 12.5 printers running non-stop