Salinization Lab
Salinization Lab
• Goals:
• To determine the relative germination
sensitivity of various crop seeds to varying
concentration of salt.
• To maintain the supplies by neat labeling and
care so that they can be used by APES
students year after year.
Salinization Lab
Independent Variables:
crop seeds, concentration of salt
Dependent Variable:
Percent germination, time to germinate
Concentrations:
Sea water 3.5%
Freshwater .005%
Dilutions of NaCl prepared by last year’s APES:
35g/L, 30g/L, 25g/L, 20g/L, 15g/L, 10g/L, 5g/L
Tapwater .005% = .05g/L Distilled Water = 0g/L
Salinization Lab Guidelines
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Use every concentration available.
Have 2 sets of samples to duplicate your experimental
results for validation.
Maximize the number of seeds within reason –
amount available, reasonable to see & count.
Transfer paper circles to each labeled petri dishes.
Don’t swamp the seeds, they’ll drown. Use enough
water to saturate the paper plus excess around the
edges only. (2.0ml)
Leave the petri dishes in sealed ziploc bags to
minimize loss to evaporation.
When observing, open the petri dishes to let air in
and resoak the paper if necessary.
Salinization Data & Observations
Conc
Date 1
Date 2
Date 3
Date 4
Date 5
Salination Lab Report
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Title, Purpose, Hypothesis, Materials
Variables: Manipulated, Responding, Controlled
Procedures – Step by Step
Data & Observations plus final percents
Conclusions
– Compare results to your hypotheses
– Use data as evidence (at least 2 #s)
– Make recommendations to the farmers of San Joaquin
valley with respect to the types of crops to plant
relative to accumulated soil salination.