1: Tao of molecules
2. Scientific Approach
3. Code of life (Basepairer)
4. Transcription and Translation
5. Protein Folding
6. Hemoglobin and Sickle Cell
7. Mitosis and Meiosis
8. Punnett and Mendel
9. Pedigrees and Exceptions to Mendel
10. Unknown Liquids
11. Tools of the Light Trade
12. Colors of light and Waste Products
Photosynthesis and
Electron Transport
System
Why we’re here:
• Solve a question about the ‘invisible world’
through experimental design and execution
• Design the experiment given tools you have
and processes you’re familiar with
• Derive the purpose and implementation of
‘controls’ for your experiment
Where we’re going
• Given: DCPIP takes electrons from Pq
Will P-synthesis happen in
DCPIP?
• How would you know?
• Add 0.2 ml/ml*final vol
• ALSO, confirm an herbicide (Diuron) is
a photosynthicide (0.15 ml/ml*)
• Give your disks time to let the herbicide
soak in
• *Ingore the change in volume adding
these produce
Question of the day/week…
X
X
WHERE does the herbicide work?
The experiment’s afoot!
• Hypothesis I: The herbicide (‘X’) acts
before DCPIP has a chance to nab
the electron
• Hypothesis II: The herbicide (‘X’) acts
after DCPIP has a chance to nab the
electron
Oh the thinks you can think!
X
X
DCPIP taking
taking -e
e- will
will
• DCPIP
do what
what to
to O
O2 prod?
prod?
do
2
How will
will -e
e- taking
taking
• How
affect DCPIP?
DCPIP?
affect
• How can you tell if
drug acts here?
• Or here?
Oh the thinks you can think!
• Two hypotheses?
• What would your experiment look
like?
• What would it TELL you if the
DCPIP…
–Stays blue
–Turns clear
“Throw her into the pond!”
• What tubes will you need?
• Work it out now
• Use the diagram
Evaluations
• What did you learn? (Can be done
individually while experiments
progress)
• What did you think of the semester
(requires lab instructor to be finished &
gone; but you can come back)