CHAPTER 16: REPRODUCTION IN PLANTS Nuclear division ● ● ● ● ● ● ● ● ● ● ● Chromosome: a thread-like structure of DNA, made up of a string of genes Gene: a length of DNA that is the unit of heredity and codes for a specific protein. A gene may be copied and passed on to the next generation Haploid nucleus: a nucleus containing a single set of unpaired chromosomes (e.g. sperm and egg) Diploid nucleus: a nucleus containing two sets of chromosomes (e.g. in body cells) In a diploid cell there is a pair of each type of chromosome and in a human diploid cell there are 23 pairs Mitosis: a nuclear division giving rise to genetically identical cells in which the chromosome number is maintained (details of stages are not required) Importance of mitosis: - Growth: in animals each tissue provides its own newcells when they are needed. - Repair of damaged tissues: for example, when you cut your skin, mitosis provides new cells to cover up cut. - Replacement of worn out cell. - Asexual reproduction: in plants Meiosis: a reduction division in which the chromosome number is halved from diploid to haploid resulting in genetically different cells (details of stages are not required) Importance of meiosis: - Meiosis is involved in the production of gametes - Meiosis maintain the continuity of life. - Meiosis results in genetic variation so the cells produced are not all genetically identical. Stem cells: unspecialised cells that divide by mitosis to produce daughter cells that can become specialised for specific functions Cancers form as a result of uncontrolled cell division Number of cells at beginning Type of cell at beginning Number of cells at end (daughter cells) Type of cell at end Number of divisions Identical or non-identical cells Used for Where it occurs Mitosis One Diploid body cell (23 pairs of chromosomes in humans) Four Meiosis One Diploid body cell (23 pairs of chromosomes in humans) Four Diploid body cell (23 pairs of chromosomes in humans) One Identical Growth and repair Everywhere except the sex organs heploid gamete (23 chromosomes in humans) Two Non-identical Producing gametes Sex organs (varies and testes in mammals) Reproduction: Formation of young one of one’s own kind is an essential character of all living things. However, this is needed for the survival of a specie. Species: A group of similar organisms which can reproduce freely in nature and produce fertile off-springs. Types of reproduction: 1. Asexual reproduction 2. Sexual reproduction Asexual reproduction It is a process resulting in the production of genetically identical offspring from one parent Sexual reproduction It is the process involving the fusion of haploid nuclei (fertilisation) to form a diploid zygote and the production of genetically different offspring Asexual reproduction: Natural vegetative propagation ● Involves the separation of a part from the parent plant ● The piece of the parent plant develops into new plant Example ● Tubere.g potato ● Rhizome e.g Ginger ● Bulb e.g Onion ● Corme.g Water Chestnut Artificial vegetative propagation ● Most artificial methods to propagate plants are based on the ability of plants to form adventitious root Example ● Cutting ● Layering ● Marcotting ● Budding ● Grafting Asexual reproductioninPlants ● Single parent ● No sexcell ● No zygote ● Mitosis occurs ● Offspring genetically same as of its parents ● Fast ● Fertilization does not occur Sexual reproduction ● Two parents ● Gametes formed ● Diploid zygote ● Meiosis occurs ● Offspring geneticallydifferent from his parents ● Slow ● Fertilization is must. Advantages and disadvantages of asexual reproduction Advantages ● ● ● ● ● No mate is needed. No gametes are needed. All the good characteristics of the parent are passed on to the offspring. This results in uniform crops and stable varieties. Where there is no dispersal (e.g. with potato tubers), offspring will grow in the same favourable environment as the parent. Plants that reproduce asexually usually store large amounts of food that allow rapid growth when conditions are suitable. Disadvantages ● ● ● There is little variation created, so adaptation to a changing environment (evolution) is unlikely. If the parent has no resistance to a particular disease, none of the offspring will have resistance. This could affect all of a crop. Lack of dispersal (e.g. with potato tubers) can lead to competition for nutrients, water and light. Advantages ● ● ● There is variation in the offspring, so adaptation to a changing or new environment is likely, enabling survival of the species. New varieties can be created, which may have resistance to disease. In plants, seeds are produced, which allow dispersal away from the parent plant, reducing competition. Disadvantages ● ● Two parents are usually needed (though not always -some plants can self-pollinate). Growth of a new plant to maturity from a seed is slow. Advantages and disadvantages of sexual reproduction Sexual reproduction Partof flower Pedicel Structure ● Stalk of a flower Function ● To support flower Receptacle (Thalamus) ● ● Swollen end of stalk ● ● Attachment for flower parts Some become fleshy after fertilization e.g apple Calyx(Sepals) ● ● Outer most whorl Green in colour ● Protect other part sof flower Corolla (petals) ● ● Second whorl Coloured ● Attract insects for pollination Androecium (stamen) (i) Filament ● Third whorl ● Male part of a flower ● Stalk that holds & another ● Supports pollen sacs (ii) Anther ● Contains two pollen sacs which contains ● Release pollen grains ● ● Pollen grains (male gamete) Forth whorl ● Female pollen of a flower Gynoecium (pistil /carpel) (i) Stigma ● Swollen structure at the end of style ● ● Receives part grains Stimulate germination of Pollen grains (ii) Style ● Stalk that connect the stigmatotheovary ● Holdstigma inasuitableposition (iii) ● Contains one or more ovules which contains egg cell (female gamete) ● Develops into fruit and ovule in to seeds Ovary Pollination The transfer of pollen grains from the anther to the stigma 1. Self-pollination ● ● Transfer of pollen grains to the stigma of same flower or different flower in the same plant. Factors that promote self-pollination: (a) Bisexual flowers with anthers and stigma maturing at the same time (b) Stigma being located directly below the anthers, allowing pollen grains to fall onto it ● ● Advantages of self-pollination: (a) Not dependent on external agents of pollination such as insects or wind (b) Less wastage of pollen and energy. During wind and insect pollination, a great number of pollen grains are lost as only a few pollen grains come into contact with a stigma of a flower of the same species. (c) Only one parent plant is required. Disadvantage of self-pollination (d) (a) is less genetic variation, (e) (b) hence the offspring is less adapted to environmental changes. 2. Cross-pollination ● Transfer of pollen grains of one flower to a flower in another plant of the same kind ● Factors that promote cross-pollination are: (a) Plants bearing only male or female flowers. These plants are called dioecious plants. (b) In plants with bisexual flowers, the anthers and the stigmas mature at different times. (c) Self-incompatibility – When a pollen grain of a flower happens to land on the stigma of the same flower or another flower on the same plant, a biochemical block prevents the pollen grain from germinating. Advantages of cross-pollination: (a) Greater genetic variation, hence the offspring has a higher chance of surviving environmental changes. (b) Offspring may have inherited beneficial qualities from both parents. Disadvantages of cross-pollination: (a) (b) (c) (d) Energy-consuming – lots of energy is required to make large amounts of pollen grains. A great number of pollen grains are wasted due to the randomness of the dispersal methods. External agents of pollination i.e. wind, insects are required. Two parent plants are required. Agents of pollination ● ● ● Wind Insect Water Feature Flower Wind pollinated flower ● Usually small ● Dull coloured ● Scentless Insect pollination flower ● Usually large ● Brightly colored ● Scented Petals ● ● Small / absent Dull coloured ● ● Large Brightly colored Nectar ● No nectar ● Produce nectar Nectar guides ● Absent ● Nectar guides present to attract and guide insects Stigma ● ● ● ● Large Feathery protrude outside Long style ● ● ● ● Small Compact / not feathery Do not protrude out Small style Stamens ● ● Long pendulous filament Anthers Protrude outside ● ● Not pendulous. Do not protrude out. Pollen grains ● ● ● ● ● ● Abundant Small Dry Light Smooth but may have Extension like "wing" ● ● ● ● ● Less in number Large Sticky Heavy Rough surface / spiky Insect pollination flower Example: Foxglove Wind pollinated flower Example: grass Fertilization It is the fusion of two different gamete i.e male and female gamete to form zygote. ● ● ● ● ● ● ● ● Pollen grain lands on stigma Absorbs water and germinate Form a pollen tube with pollen tube nucleus following generative nucleus Penetrates stigma by secretion of some enzymes Enters ovule through micropyle. Generative nucleus divides to form two male gametes. Tube nuclear degenerates One male gamete fuses with the ovum to form zygote. ● ● Other male gamete fuses with the definitive nucleus to form endosperm nucleus. Zygote will divide and develop into embryo. Flower Parts Ovary Ovary wall Ovule Ovule wall (integuments) Ovule stalk Endosperm nucleus Zygote Sepals Style Stamen Stigma Petals Post fertilization changes Fruit Fruit wall or pericarp Seed Seed coat or testa or tegmen Seed stalk (funicle) Endosperm Embryo ● Plumule ● Radicle ● Cotyledons May persist/ enlarge Wither and fall off Dispersal of fruit and seeds Importance It is necessary in order to ● ● ● ● ● Avoid over crowding Avoid competition for food Avoid competition for light Enable plants to colonise new and favourable habitats Reduce the spread of diseases Dispersal is carried out by the following methods 1. 2. 3. 4. By wind By animals By water By explosive mechanism Dispersal by Wind Animal Features Modeofaction Example ● ● Small Light ● Can float in the air ● ● Orchid seed Dendlion ● ● ● ● ● ● Large Flattened wing like Parachute like Projection Fine hairs Surface are a enlarged ● ● Carried in the wind Increases air resistance or buoyanay in air ● ● Angsana fruit sycamore ● ● ● ● Sweet Succulent Brightly coloured Scented ● ● Eaten by animals Undigest seeds are passed out ● ● Tomato Apple ● ● Hooked Hairy fruits ● Adhere onto the fur or skin of animals passing by ● ● Mimosa Bur Structure of a seed ● ● ● ● ● ● Structures Seed Stalk Hilum Micropyle Testa Embryo has a) Plumule b) Radicle c) Cotyledon ● ● ● ● ● ● ● ● Function Attaches the seed to the pericarp The scar left by the seed stalk A small hole for entry of water Thick protective seed coat The first arise shoot The first arise root The seed leaves Food storage tissue Endosperm Germination When suitable environmental conditions are available, the seed will germinate ● ● ● Water enters the seed through the micropyle Testa softens and splits Enzymes produced by the cotyledons digest the food in endosperm. Starch → maltose → glucose Proteins → amino acid Fats → fatty acids + glycerol ● Simple digested substances translocated to the radical and plumule ● ● ● ● ● ● The seeds shrinks and its dry mass decreases at first because of tissue respiration First foliage leaves develop Because of photosynthesis dry mass increases The glucose is used in respiration to provide the energy for the process of growth. The amino acids are used to build up proteins in the cytoplasm of the new cells. The fatty acids and glycerol recombine to form fats which are used to make important components of cell membranes. Fats also provide a considerable amount of energy. Conditions for germination ● ● ● Sufficient water Suitable temperature Adequate oxygen supply Investigating the condition needed for germination Small seeds such as peas or mustard will grow on wet cotton wool in a test tube. Figure below shows four tubes set up to investigate the conditions needed for the seeds to germinate. ● ● ● ● ● Tube A: seeds on wet cotton wool, maintained at 20°C (room temperature) Tube B: seeds on dry cotton wool, maintained at 20°C Tube C: seeds in boiled water, with a thin surface layer of oil; maintained at 20°C The water in tube C has been boiled to remove dissolved oxygen. The layer of oil (e.g. cooking oil) keeps out oxygen from the air. Tube D: seeds on wet cotton wool, placed in a refrigerator at 4°C After a few days the seeds in the control tube (A) will start to germinate. There will be no germination in tubes B or C. The seeds in tube D may eventually start to germinate, but much more slowly than in tube A. Reproduction in Mammals Sex Hormones ● ● ● ● At puberty, the pituitary gland starts to stimulate the primary sex organs; the testes in males and the ovaries in females. Sex hormones – testosterone in males and oestrogen in females are released into the bloodstream. They only affect the target organs which have receptors which can recognize them. Causes secondary sexual characteristics such as the growth of pubic hair and maturation of sexual organs. Male reproductive system The male nuclei involved in the process of human sexual reproduction are located within male gametes (sex cells) called sperms. The male reproductive system is designed to manufacture sperms and to deliver them to the place where one of them will be able to fuse with a female nucleus. Reproductive organ Testis Structure Function ● ● ● Paired Oval in shape Suspended in scrotum ● ● Produce sperms Produce testosterone Epididymis ● Coiled tube ● Store sperms temporarily Scrotum ● ● Sac formed by the Skin toenclose testes ● Protect the testes Sperm duct ● Connects the epididymis to the urethra ● Transport sperm to urethra Urethra ● Tube that runs through the penis ● Carries both urine and semen at different times, through the penis Penis ● An organ of spongy erectile tissue ● Deposits semen into the vagina of female Reproductive glands Prostate gland ● Seminal vesicle ● ● ● ● Largest among three Single gland located at the part where the urethra and two sperm ducts join ● A pair of glands that open directly into the sperm ducts ● ● ● Produces thin white alkaline fluid containing many enzymes Helps to activate sperms Produces thick and clear fluid containing mucus, amino acids and Fructose provides energy Cowper’s gland ● ● ● ● Open directly into the urethra ● to sperms ● ● Produces clear viscous fluid Carries sperms released before ejaculation. Sperms along with the nutritional fluid from glands is called semen. The ejection of semen out of the body is called ejaculation. During one ejaculation, about 500 million sperms are secreted. Female reproductive system The female nuclei which are involved in the process of sexual reproduction are located in the female gametes called OVA (singular: ‘ovum’). The functions of the female reproductive system are: (i) (ii) To produce ova and ensure that they are fertilised by the male gametes To protect and nourish the embryo until it is born. Reproductive organ Ovary Structure ● Paired ● Oval in shape ● Attached dorsal wall of abdomen Function ● Releases ovum every 28 days ● Produces oestrogen and progesterone Oviduct (fallopian tube) ● ● A narrow tube with a funnel shaped opening close to ovary ● Uterus ● ● Hollow Muscular pear shaped organ ● Transfer ova from ovary into uterus Site of fertilization Protects and nourishes a developing baby Cervix Vagina ● Richly supplied with blood ● ● Helps keep the baby in the uterus until it is born ● A ring of muscles at the lower end of the uterus Opens into the vagina ● ● A tube with thinner walls. The site where sperms are deposited ● Serves as an opening for the entry of penis Serves as birth canal ● Comparison of male and female gametes Characteristics Chromosome number Size Ovum(egg) 23 ● Large (0.1) mm Sperm 23 ● Smaller ( 0.6mm) Shape ● ● ● Spherical Inner plasma membrane Outer protein coat ● ● ● Head with nucleus Mitochondria in middle Tail at the end Mobility ● Immotile ● Motile Nucleus ● Much larger ● Smaller Cytoplasm ● A lot of cytoplasm ● Very little Reserved food ● Cytoplasm as food reserves ● No reserves food Released ● One at a time about 28 days ● In millions at each ejaculation Type of chromosome ● ● Nucleus contains Only X chromosome always Nucleus contains either X or a Y chromosome ● Release of gametes ● Internal ● Expelled Gamete Size Mobility Number Sperm Smaller Very mobile – use its tail Many more (300,000,000 Egg Larger Immobile – moved by peristalsis Fewer and limited Menstrual cycle ● ● ● ● In female occurs after puberty till menopause Discharge lasts for about 3-5 days Cycle repeats after 28 days Regulate by hormones 1. Follicle stimulating hormone (FSH) is secreted by the pituitary gland. It causes an egg to mature in an ovary and it stimulates ovaries to release hormone oestrogen 2. Oestrogen is secreted by the ovaries. It stops FSH being produced - so that only one egg matures in a cycle and it stimulates the pituitary gland to release hormone LH. 3. Luteinizing hormone (LH): is also secreted by pituitary gland and causes mature egg to be released from ovary. 4. Progesterone is a hormone secreted by ovaries. It maintains the lining of the uterus during the middle part of the menstrual cycle and during pregnancy. Phases Menstruation (days 1 – 5) ● ● ● ● ● Corpus luteum disintegrates Progesterone secretion reduces FSH and LH stimulate follicles to ripen Uterus lining disintegrates Menstrual flow through vigina which contains o Dead egg cell o Blood o Mucus o Water o Disintegrated lining Pre-ovulation phase (days 6-13) ● ● ● ● Graffin follicle develops Other follicles disintegrate Increase in LH causes follicle to mature fully Uterus lining becomes thick and vascularised by oestrogen Ovulation (days 14) ● ● ● ● ● Graffin follicle burst Egg is released from its follicle from ovary LH causes ovulation LH also causes the formation of corpus Luteum Corpus luteum secrets progesterone Post-ovulation (day 14-28) ● ● ● Uterus lining continues to thickness Egg dies if not fertilized Corpus luteum begins to shrink ● Decrease in level of estrogen and progesterone Fertilization ● ● ● ● Is the fusion of sperm with the egg to form a zygote. Occurs during sexual intercourse Only one sperm fertilizes the egg and form zygote. Zygote divide mitotically and form embryo Implantation ● ● ● ● Embryo moves to the uterus Embryo implants in uterus lining in 6 days after fertilization The embryo starts to absorb nutrients from mother Placenta is formed three months after pregnancy. Gestation period ● ● The period between fertilization and birth is called the gestation period Takes 9 months in humans Placenta Is the attachment of fetus to the mother uterus lining by numerous finger like villi. Functions of placenta Material Useful Substances i. Glucose ii. Amino acid iii. Vitamins iv. Minerals v. Water vi. Oxygen Waste substances i. Urea Function ● Pass from the placentain to the embryo ● Pass from embryo back to mother’s blood. ● Stops reaching the embryo but some viruses can pass across the placenta and affect the fetus ii. Toxins iii. Chemicals Antibodies ● Diffuse from mother’s blood into embryo Hormones progesterone ● Maintains uterine lining in healthy state. ii. CO2 Harmful substances i. Pathogens Two blood systems ● ● Allows two blood system to function at different blood pressure since the two blood types do not mix. In the placenta, the blood capillaries of the feats are separated from the mother’s blood system by only a thin layer of tissue. Fetal blood system is not continuous with the mother’s blood system because. o The blood pressure system of mother would kill the fetus as it is much higherthan of fetus o Blood group of the fetus may not be the same as the mother, so avoiding agglutination ● Umbilical Cord “Attaches the fetus to the placenta” It contains: ● ● Umbilical arteries Transports material from the fetus to the placenta e.g i. Deoxygenated blood ii. Metabolic waste products Urea ● ● ● ● ● ● Umbilical vein Transplants material from the placenta to the fetus e.g i. oxygenated blood ii. food Substances glucose amino acid vitamins water ● minerals Amniotic Sac (amnion) “A sac-like membrane around the embryo filled with a watery fluid called amniotic fluid . ● ● ● ● ● It supports and cushions the fetus Shock absorber Protects against physical injury Allow fetus to move freely. During birth, it lubricates and reduces friction in vigina.