Introduction
The MEK-6510J/MEK-6510K Automated Hematology Analyzer is designed for
simultaneous 19-parameter measurement. You only need to put the sampling
nozzle into a sample container which contains whole blood and press the [ Count]
switch. Results and data are displayed on a color LCD screen. An optional printer
allows full reports. The analyzer automatically cleans the remaining blood on the
sampling nozzle, so it is safe and there is no risk of touching the blood. The
analyzer has self-check, quality control and other programs to provide reliable data
management system.
Measured Parameters
WBC: White Blood Cell Count
RBC: Red Blood Cell Count
HGB: Hemoglobin Concentration
HCT: Hematocrit (%)
MCV: Mean Corpuscular Volume (fL)
MCH: Mean Corpuscular Hemoglobin (pg)
MCHC: Mean Corpuscular Hemoglobin Concentration (g/dL)
PLT: Platelet Count
LY%: Lymphocyte Percent
MO%: Monocyte Percent
GR%: Granulocyte Percent
LY: Lymphocyte Count
MO: Monocyte Count
GR: Granulocyte Count
RDW-CV: Coeffcient of Variation of the Red Blood Cell Distribution Width
RDW-SD: Standard Deviation of the Red Blood Cell Distribution Width
PCT: Platelet Crit
MPV: Mean Platelet Volume
PDW: Platelet Distribution Width
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Turning On the Power
1. Confirm that the analyzer, printer (optional), and hand-held bar code reader
(optional) power cords are connected to grounded power outlets.
2. Set the printer power switch ON.
3. Press the [Main power] switch on the rear panel to ON. The green [Main
power lamp] on the front panel lights
4. Press the [Power] key on the front panel ON. The green [Power lamp] lights
and the screen illuminates within 15 to 30 seconds. Cleaning of the fuid
path, priming and circuit self-check are automatically performed
After priming operation is completed, the READY screen appears. The analyzer
is ready for counting .
Checking the Date and Time Settings
After turning the analyzer ON, date and time must be checked.
Cleaning the Fluid Path After Turning the Power On (PRIME ON INSTALL)
The fluid path inside the analyzer must be cleaned after installation or long-term
storage. Perform PRIME ON INSTALL on the OPERATIONS screen.
Checking Daily Accuracy
• Count the diluent to measure background noise.
• Count the MEK-3D hematology control to check accuracy.
Measuring Hematology Control
For checking accuracy, use hematology control which has the same
conditions as human blood. Confrm that the obtained sample data is within
the acceptable range on the assay sheet attached to the hematology control.
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Changing Settings
Assigning an ID to a Sample
All samples must have an ID. When you set an ID, the analyzer automatically
assigns it to the next counted sample and increments the ID for each sample after
that .
Displaying the ID Screen
1. Press the ID key on the MENU or RESULTS screen, or the SET key or ID
box on the READY screen to display the ID screen.
2. Press the OK key to return to the previous screen. (i.e. If the ID screen
is displayed from the READY screen, the screen returns to the READY
screen.)
Using Bar Codes
The optional hand-held bar code reader (Keyence BL-N60RK or equivalent) can
be used to read the sample bar code label to establish the Sample ID.
To enter the ID by using the hand-held bar code reader, display the READY, ID,
RESULTS or ID EDIT screen and read the bar code of the sample.
Setting Date and Time
1. Press the SETTINGS key on the MENU screen.
2. Press the DATE & TIME key on the SETTINGS screen to display the DATE
& TIME screen.
3. Select the date format in <Date format>
4. Touch the setting value or use the arrow keys to move the cursor to the
setting value you want to change.
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Setting Auto Priming/Cleaning
To maintain the optimum condition, you can set the analyzer to automatically
perform priming or cleaning at a certain time of the day. You can schedule auto
priming/cleaning for up to 4 times a day. To change the settings, the type of user
must be either lab technician or service.
1. Press the SETTINGS key on the MENU screen.
2. Press the AUTO CLEANING key on the SETTINGS screen to display the
AUTO CLEANING screen.
3. Set the time for the schedule.
4. Select “Clean”, “Prime” or “No” from the selection list for each schedule.
5. Press the OK key to return to the SETTINGS screen.
Using Reagent Management
You can set the analyzer to display a warning when the reagent or waste reaches
the warning level.
1. Press the OTHER key on the MENU screen to display the OTHER screen.
2. Press the MANAGE REAGENT key on the OTHER screen.
3. Select “On” in <Use reagent management> to use the reagent management
function.
To change current level, warning level and bottle size of a reagent:
i) Select the reagent or waste by pressing the reagent key.
ii) Press the SET key to display the setting screen for the selected reagent.
iii) Touch the setting value or use the arrow keys to move the cursor to the
setting value you want to change.
iv) Enter the value with the numeric keys and press the Enter key to register
the value.
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v) Press the OK key to return to the previous screen.
5. Press the OK key to return to the OTHER screen.
Measurement
Dilution Mode
There are five dilution modes:
• Normal mode
In normal dilution mode, 30 µL sample volume is measured.
• Pre-dilution mode
For pre-dilution blood measurements, you can specify the pre-dilution blood
measuring volume (10 µL or 20 µL)
• High or higher dilution mode
When a blood sample’s WBC seems to be high, the sample can be measured
in high or higher dilution mode.
• Low dilution mode
When a blood sample’s WBC or PLT seems to be low, the sample can be
measured in low dilution mode. The low dilution mode reduces the recount
and measurement data is stable.
Sample Type
With this analyzer, you can specify the sample type before each measurement.
Measuring a Venous Sample in Normal Dilution Mode
Preparing a Venous Sample
1. Put 2 mL of collected whole blood in a sample container which contains
anticoagulant.
2. Gently shake the covered sample container up and down more than 30 times.
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Measuring a Venous Sample
1. On the READY screen, check the <ID> and <Sample type> setting and
check that “Normal” is selected for <Dilute mode>.
2. Put the sampling nozzle into the sample container of the sample blood so
that the tip of the sampling nozzle comes near but does not touch the bottom
of the sample container.
3. Press the [ Count] switch or key. The sample is aspirated and measurement
starts. The “Measuring” message appears on the screen.
Measuring a Pre-Dilution Sample
Preparing a Pre-Dilution Sample
You can select pre-dilution blood measuring volume (10 or 20 µL) on the
MEASUREMENT screen of the SETTINGS screen.
1. On the READY screen, check the <ID> and <Sample type> setting.
2. Select “Pre-dil” for <Dilute mode>. The DISPENSE key appears on the
Screen.
3. Put the sampling nozzle into an empty sample cup so that the tip of the
sampling nozzle touches near the rim of the sample cup as shown in the
fgure.
4. Press the DISPENSE key on the READY screen or the [ Dispense] key on
the front panel. A 2 mL of the diluent is dispensed into the sample cup.
5. Collect 10 or 20 µL of whole blood with a capillary tube.
6. Put the pre-dilution blood into the sample cup with the dispensed diluent. Be
careful not to create any bubbles.
7. Gently shake the sample cup up and down more than 10 times.
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Measuring a Pre-Dilution Sample
1. Put the sampling nozzle into the sample cup containing the pre-dilution
sample so that the tip of the sampling nozzle comes near but does not touch
the bottom of the sample cup.
2. Press the [ Count] switch or key. The sample is aspirated and measurement
starts.
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