CERTIFICATE OF ANALYSIS ATCC Number: VR-740™ Lot Number: 70053995 Classification: Coronaviridae, Coronavirus Agent: Human coronavirus 229E Strain: 229E Passage History: Submission laboratories: Human Embryonic Kidney (HEK) (2), WI-38 (11), Human Embryonic Lung Fibroblasts (RU-1) (8), WI-38 (1) ATCC: MRC-5 (1), MRC-5 (ATCC® CCL-171™) (4) Fill Volume: 1.0 mL Product Format: Fluid and cell lysate from infected cultures Expiration Date: Not applicable Storage Conditions: - 70°C or colder Test / Method Specification Result Viability (Infectivity)* PFU, IFU, TCID50 or CEID50 ≥ 5 × 103 per mL Pass Authentication** Virus identity verified by Immunofluorescence, ELISA, and/or sequencing Pass Test for Mycoplasma Contamination Broth and agar culture (direct method) DNA detection by PCR of test article nucleic acid No growth None detected No growth None detected Sterility test (BacT/ALERT 3D) iAST bottle (aerobic) at 32.5°C, 14-day incubation iNST bottle (anaerobic) at 32.5°C, 14-day incubation No growth No growth No growth No growth *Titer notes: 5.0 × 107 TCID50/mL in 8 days on MRC-5 cells (Homo sapiens lung fibroblast cells) at 35°C with 5% CO2 as determined by cytopathic effect. Tested on 06OCT2022. **Authentication notes: Genomic authentication was performed by Next Generation Sequencing (NGS) and results meet requirements of ≥ 1000 bp with ≥ 98% homology to GenBank number AF304460.1 (Human Coronavirus 229E, complete genome) Sonia Bjorum Brower Digitally signed by Sonia Bjorum Brower Date: 2022.12.13 16:26:30 -05'00' Sonia Bjorum Brower Technical Manager or designee, ATCC Federal Solutions 13 DEC 2022 ATCC hereby represents and warrants that the material provided under this certificate is pure and has been subjected to the tests and procedures specified and that the results described, along with any other data provided in this certificate, are true and correct to the best of the company’s knowledge and belief. This certificate does not extend to the growth and/or passage of any living organism or cell line beyond what is supplied within the container received from ATCC. ATCC 10801 University Boulevard Manassas, VA 20110-2209 USA www.atcc.org Template Doc ID: 31194 - Page 1 of 2 Template Revision: 6 800-638-6597 or 703-365-2700 Fax: 703-365-2750 E-mail: tech@atcc.org or contact your local distributor Template Effective Date: 02/21/2020 CERTIFICATE OF ANALYSIS ATCC Number: VR-740™ Lot Number: 70053995 This product is intended to be used for laboratory research use only. It is not intended for use in humans, animals, or for diagnostics. Appropriate Biosafety Level (BSL) practices should always be used with this material. Refer to the Product Information Sheet for instructions on the correct use of this product. ATCC products may not be resold, modified for resale, used to provide commercial services, or to manufacture commercial products without prior written agreement from ATCC. © 2017 ATCC. The ATCC trademark and trade name are owned by the American Type Culture Collection. ATCC 10801 University Boulevard Manassas, VA 20110-2209 USA www.atcc.org Template Doc ID: 31194 - Page 2 of 2 Template Revision: 6 800-638-6597 or 703-365-2700 Fax: 703-365-2750 E-mail: tech@atcc.org or contact your local distributor Template Effective Date: 02/21/2020
