Media Differential media – media that can help separate bacteria based on a metabolic process Selective media – media that has an ingredient that can encourage the growth of some bacteria while inhibiting the growth of other bacteria Media – material that used for the growth of microorganisms Inoculate – the process by which microorganisms are added to media Colony – group of genetically identical bacteria Colony Morphology – 8 characteristics (shape, margin, elevation, size, texture , appearance, pigmentation, optical property) MSA Mannitol Salt Agar – isolates and differentiates for Staphylococcus spp. Differential Selective mannitol -> fermented -> turns yellow growth in 7.5% NaCl = Gram (-) bacteria present pH indicator – phenol red MSA https://www.youtube.com/watch?v=ON4qQafGcfc MacConkey Agar – isolates and differentiates Gram (-) bacteria Differential Selective Lactose -> fermented -> turns hot pink growth in bile salt and crystal violet = Gram (-) MacConkey https://www.youtube.com/watch?v=f0pgH7adv7A Blood agar – isolates and cultivates fastidious bacteria & differentiates based on hemolysis Differential Selective Complete breakdown hemoglobin = Beta growth in blood nutrient = fastidious bacteria Partial breakdown of hemoglobin = alpha No breakdown of hemoglobin = gamma Starch Agar – differentiates amylase (+) bacteria Reagent : Iodine � Halo = Amylase (+) No Halo = Amylase (-) EMB - Eosin Methylene Blue Agar – isolate Gram (-) bacteria and differentiate lactose fermentation Differential Selective High Lactose Ferment = (+) Bright green/blue black growth in presence Eosin & Methylene blue = Gram (-) Slow Lactose ferment = purple No Lactose ferment = pink/colorless This study source was downloaded by 100000809546766 from CourseHero.com on 11-27-2022 18:25:04 GMT -06:00 https://www.coursehero.com/file/144574199/MicroLab-Study-sheetdocx/ Media Phenol red broth – differentiate carbohydrate fermentation and gas producers Pink = (-) carb fermenter Yellow = (+) carb fermenter Gas production (+) or negative (-) if bubble shows SIM tube – Sulfide Indole Motility - Differentiate if produces hydrogen sulfide, indole and has motility H2S (+) = Black H2S (-) = Not Black Add /reagent Kovac’s Indole (+) = Red Indole (-) = Yellow Motility (+) = cloudy away from stab line Motility (-) = growth only at stab line This study source was downloaded by 100000809546766 from CourseHero.com on 11-27-2022 18:25:04 GMT -06:00 https://www.coursehero.com/file/144574199/MicroLab-Study-sheetdocx/ Powered by TCPDF (www.tcpdf.org)
