Microscope Introduction and Letter e lab
So this is our microscope introduction lab. First thing is that you always want to carry the microscope
with one hand holding that curved arm and one hand under the base of the microscope. So two hands
when you carry it back and forth from the counter to the table and so on just quickly going over the
different parts of the microscope.
First one is the eyepiece. The eyepiece is what contains the ocular lens it's where you look into the
microscope. The course knob is the larger knob when you turn it it moves. That eyepiece up and down
so you can kind of zoom in and out of your viewing. The smaller of the two knobs is called the fine knob
it sharpens the image. So you use the coarse knob to focus in on what you're looking for. And then the
fine knob makes it just a little bit more clear. The light source is that box that controls the light? And on
that light source is what we call a diaphragm. It's a little wheel that turns on the light and makes it
brighter or dimmer. So it varies how much light gets through. Our microscope stage is round and it's
where the microscope slide sits. The stage Clips keep the slide in place when you're viewing and the
objective lenses. We have 3 we have a red one a yellow one and a blue one they rotate so you can get
closer to the image. Thee total magnification of what you're viewing is found by multiplying the power
of the ocular lens in the eye piece by the power of the objective lens. So in this case if the ocular lens is
10 and the objective lens is 4 we would do 10 x 4 which gives us 40. On your worksheet or eyepieces 10
the scanning objective. The red lens has a magnification of 4 so when you're using the red lens you see
things 40x their original size. When we use low power. The yellow objective lens is 10 x 10. So we're
actually seeing things at a 100x their original size. The high-power the blue objective lens is 40. So when
we multiply those together we get a total magnification of 400x the original size. Now, this is important
because the different powers of magnification allows us to see different things. We can see better
details with higher powers of magnification, but we can't see as much of the image. So if you look at the
two images on the left, we see most of the bird and it's head versus zooming in close eye on its beak.
The left side is a lower power magnification. You see more of the image. The right side is higher power
magnification. We see more detail but less of the whole thing. So which of these two images of the
bottom will be viewed So which of these two images of the bottom will be viewed at a higher power
magnification answer would be the one on It's zoomed in a lot closer a lot more detail and our objective
lens that we used for high power is blue. Now I'm going to show you what our microscope can do using
the letter e lab that we did in class. I'm switch views here and we're going take a look at. microscope so
if I put letter e under the microscope, this is what I would see much more zoomed in but you'll notice
the E is upside down and backwards. It's completely flipped around upside down and backwards. Now if
I want to get a closer view because I'm very interested in all of these little cracks in the ink. I would
switch to the yellow objective lens low power and then I have to use my course knob to kind of zoom in
and focus and then I use my fine knob to sharpen it so it's not so blurry now. I see a lot more detail. But I
can't see all of the E doesn't fit. It's a little too big. if I want even further see even more detail, I'm going
to switch to my high-power my blue objective lens now see how blurry it is. I can't use the coarse knob
here. I can only use the fine knob to sharpen the image and look at all those little ink splotch. Kind of
looks like splatter paint. Okay, because the paper itself isn't as smooth as you think it is. So again, I
found my e in the red scanning lens. Okay, so I'm going to zoom out a little bit so you can kind of see
what it looks like to find the E. All right, when I put the E under I had a zoom in with the course knob
until it comes into focus. Then move into the yellow. Adjust a bit, so it's clear and sharp. And then I want
even more detail. Switch right to the blue use my fine knob only make it clear.