CONTINUED MEETING & DISCUSSION
BIOSENSOR TRYPANOSOMIASIS
& BIOSENSOR
April H Wardhana, SKH, MSi, PhD
Hasim Munawar, Ssi, MPhil
25th August 2020
Balai Besar Penelitian Veteriner – Bogor
Badan Penelitian dan Pengembangan
Pertanian
Kementrian Pertanian
PROGRESS BIOSENSOR OF TRYPANOSOMIASIS
Meeting & Discussion, 25th August 2020
Wardhana24id@yahoo.com
RESULTS OF BIOSENSOR FOR TRYPANOSOMIASIS
Carbon electrode gave a better response than gold electrode. The CE was selected for
further analyses
140%
120%
100%
%I
80%
60%
40%
20%
0%
1/64
1/32
1/16
1/8
1/4
1/2
Diluted positive Sera of anti Trypanosoma evansi
Carbon electrode (CE)
Gold electrode (GE)
Diluted positive sera of Trypanosoma evansi
Carbon electrode (CE)
Gold electrode (GE)
Meeting & Discussion, 25th August 2020
Wardhana24id@yahoo.com
RESULTS OF BIOSENSOR FOR TRYPANOSOMIASIS
Diluted sera (1/64) gave similar responses
among CE, CE + protein T. evansi and CE +
protein standard.
It could be FALSE NEGATIVE
Diluted sera (1/2) gave similar responses
among CE, CE + protein T. evansi and CE +
protein standard.
It could be FALSE POSITIVE
Diluted positive sera of Trypanosoma evansi
Diluted sera (1/2) dan (1/64) were not
included for further analyses
Carbon electrode (CE)
Carbon electrode + Protein of T. evansi
Carbon electrode + Protein Standard/Belgium
Meeting & Discussion, 25th August 2020
Wardhana24id@yahoo.com
RESULTS OF BIOSENSOR FOR TRYPANOSOMIASIS
Serological test
BIOSENSOR
Negative
sample
Positive
sample
ELISA test
The positive sera gave response
almost twice compared to the
negative sera
Diluted positive sera of Trypanosoma evansi
Meeting & Discussion, 25th August 2020
Wardhana24id@yahoo.com
FUTURE WORKS
Improvement the Biosensor for Trypanosomiasis by finding the proper crosslinkers. We
will try many crosslinker to find out the best one.
Selecting the best isolate of Trypanosoma evansi that are able to recognize all parasites.
We need to characterize the protein and conduct ELISA.
Optimizing the protein concentration for coating on the electrode, including
determining the false positive and negative of samples
Performing stabilization, sensitivity and specificity test using field samples (antibody)
from cattle, buffalo and horse.
If possible, attending training in Norway to learn more detail of Biosensor. Discussing
with people in R and D or some experts if we want to manufacture the sensor.
Applying a proposal for getting financial support. For example : Marie Curie Sponsorship
Developing Biosensor for screening of Trypanosoma based on images/photoes
Meeting & Discussion, 25th August 2020
Wardhana24id@yahoo.com
SHORT MEETING & DISCUSSION
TRYPANOSOMIASIS
& BIOSENSOR
April H Wardhana, SKH, MSi, PhD
Hasim Munawar, Ssi, MPhil
18th August 2020
Balai Besar Penelitian Veteriner – Bogor
Badan Penelitian dan Pengembangan
Pertanian
Kementrian Pertanian
TRYPANOSOMA spp.
Undulating membrane
Free
Flagellum
Kinetoplast
Volutin
Granules
Pellicle
Nucleous
Meeting & Discussion, 18th August 2020
Cytoplasm
Wardhana24id@yahoo.com
PROPERTY
My institute, The Indonesian Research
Center for Veterinary Science has 416
isolates of
T. evansi
preserved into liquid nitrogen
Those isolates were collected
from different regions
throughout Indonesian
archipelago
Some isolates have been
characterized using molecular
technique and examined their
virulence level including
comparing genotype and fenotype
Meeting & Discussion, 18th August 2020
Wardhana24id@yahoo.com
GENETIC DIVERSITY OF T. evansi IN INDONESIA
Sumba Timur
Sumba Timur
Sumba Timur
Pandeglang
Lebak
Bangkalan
Sukabumi
Muntilan
Sumbawa
High virulence
Pandeglang
Pemalang
Moderat virulence
Low virulence
Pemalang
(Source : Sawitri et al. 2017)
Meeting & Discussion, 18th August 2020
Wardhana24id@yahoo.com
MORFOLOGICAL DIFFERENTIATION OF Trypanosoma
spp
Similar to
Trypanosoma evansi
Meeting & Discussion, 18th August 2020
Wardhana24id@yahoo.com
MORFOLOGICAL DIFFERENTIATION OF Trypanosoma
spp
Similar to
Trypanosoma evansi
Meeting & Discussion, 18th August 2020
Wardhana24id@yahoo.com
MORFOLOGY OF Trypanosoma evansi
Photo :
Personal document
Meeting & Discussion, 18th August 2020
Wardhana24id@yahoo.com
MORFOLOGY OF Trypanosoma evansi
Laboratory colony
Trypanosoma evansi (1000 X)
Meeting & Discussion, 18th August 2020
Photo : Personal document
Wardhana24id@yahoo.com
MORFOLOGY OF Trypanosoma evansi
Laboratory colony
Trypanosoma evansi (1000 X)
Meeting & Discussion, 18th August 2020
Field sample - horse
Photo : Personal document
Wardhana24id@yahoo.com
MORFOLOGY OF Trypanosoma evansi
Field sample - mice
Meeting & Discussion, 18th August 2020
Wardhana24id@yahoo.com