!"#$%&$#'%!"#$"%&!&'
PAP-Method
Enzymatic Colorimetric Test for Uric Acid with Lipid
Clearing Factor (LCF)
()*+),-%./0-1
10690
!"#$%
10691
!&'(%
4 x 30 ml
4 x 100 ml
Complete Test Kit
Complete Test Kit
2-3456%1,2
Determination of uric acid by reaction with uricase. The formed H2O2
reacts under catalysis of peroxidase with 3,5-dichloro-2hydroxybenzenesulfonic acid (DCHBS) and 4-aminophenazone (PAP)
to give a red-violet quinoneimine dye as indicator.
"-)*3/57%(8/7*/9:uricase
Uric acid + O2 + 2 H2O !!!!" allantoin + CO2 + H2O2
peroxidase
2 H2O2 + DCHBS + PAP !!!!" quinoneimine + HCl + 4 H2O
$573-731;%"-),-73%$5<951/3/57%/7%34-%=-13
>%?%@A%<:%58%>%?%BAA%<:%C70D<-%8-),-73
!")*%
Phosphate buffer (pH 7.0)
4-Aminophenazone
DCHBS
Uricase
Peroxidase
!+*(%
@%<:%.3)76)86
Uric acid
50 mmol/l
0.3 mmol/l
4 mmol/l
> 200 U/l
> 1000 U/l
8 mg/dl or 476 µmol/l
"-),-73%(8-9)8)3/57
!")*% and !+*(% are ready for use.
"-),-73%.3)E/:/3D
The reagents are stable, even after opening, up to the stated expiry
date when stored at 2...8°C. Contamination of the reagents must be
absolutely avoided.
Stored at 15...25°C, 9853-*3-6% F85<% :/,43, !")*% is stable for 2
weeks.
.9-*/<-7
Serum, heparinised plasma or EDTA-plasma, urine.
Dilute urine 1+10 with dist. water.
G53-:
Lipemic specimens usually generate turbidity of the sample
reagent mixture which leads to falsely elevated results. The
!"#$% &$#'% !"#$"%&!&' test avoids these falsely elevated results through its built-in Lipid-Clearing Factor (LCF). The LCF
clears up totally a turbidity caused by lipemic specimens.
&11)D
Wavelength:
Optical path:
Temperature:
Measurement:
$):*H:)3/57%5F%34-%!8/*%&*/6%$57*-738)3/57
.-8H<;%(:)1<)
#A Sample
C = 8 x !!!!!!
[mg/dl]
or
#A !+*(%
#A Sample
C = 476 x !!!!!
#A !+*(%
[µmol/l]
#A Sample
C = 88 x !!!!!!
#A !+*(%
[mg/dl]
#A Sample
C = 5235 x !!!!!
#A !+*(%
[µmol/l]
!8/7or
(-8F58<)7*-%$4)8)*3-8/13/*1
Linearity: the test is linear up to an uric acid concentration of 20 mg/dl
or 1190 µmol/l. Dilute samples with a higher concentration 1 + 1 with
physiological saline (0.9%). Multiply the results by 2.
Typical performance date can be found in the Verification Report,
accessible via
www.human.de/data/gb/vr/su-urac.pdf or
www.human-de.com/data/gb/vr/su-urac.pdf
"-F-8-7*-%I):H-1%3
Men
3.4 - 7.0 mg/dl
Women
2.4 - 5.7 mg/dl
Urine
250 - 750 mg/24h
or
or
or
200 - 420
140 - 340
1.5 - 4.5
µmol/l
µmol/l
mmol/24h
JH):/3D%$57385:
All control sera with uric acid values determined by this method can be
employed.
We recommend to use our K!2&="LM control sera based on animal
serum or .C"L'L. based on human serum.
&H35<)3/57
Proposals to apply the reagents on analysers are available on request.
Each laboratory has to validate the application in its own responsibility.
G531. The test is not influenced by hemoglobin values up to 100 mg/dl or
by bilirubin values up to 20 mg/dl.
2. The standard contains sodium azide (0.095%) as preservative. Do
not swallow. Avoid contact with skin and mucous membranes!
"-F-8-7*-1
1. Barham, D., Trinder P., Analyst NO, 142 (1972)
2. Fossati P. !"#$%&, Clin. Chem. PQRP, 227 (1980)
3. Thefeld, L. !"#$%, Dtsch. med. Wschr. NS, 380-384 (1973)
520 nm, Hg 546 nm
1 cm
20...25°C or 37°C
against reagent blank (Rb). Only one reagent blank
per series is required.
(/9-33/7,%.*4-<Please use only the standard recommended by HUMAN (enclosed in
the kit).
Pipette into cuvettes
Sample / !+*(%
!")*%
Rb
Sample or !+*(%
--1000 µl
20 µl
1000 µl
Mix, incubate 10 min. at 20...25°C or 5 min. at 37°C. Measure the
absorbance of the sample / !+*(%%against the reagent blank within 15
min. (#A).
SU-URAC
INF 106901 GB
04-2004-14
!
Human Gesellschaft für Biochemica und Diagnostica mbH
Max-Planck-Ring 21 - D-65205 Wiesbaden - Germany
Telefon: +49 6122 9988 0 - Telefax: +49 6122 9988 100 - eMail: human@human.de