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Determination of the actual cell size using an ocular micrometer eyepeace graticule and object stage micrometer

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https://www.slideshare.net/MrOakes/as-biology-lesson-2-measuring-cells
19.
• Figure 4.6. Cells of the onion epidermis as viewed
at x400 magnification with the same graticule in
the eyepiece of the microscope
Remember, we know that at this magnification,
each division of the eyepiece graticule represents
2.67μm 62 divisions.
• Here, two cells span 62 divisionson the eyepiece
graticule. Thisrepresents 2.67 x 62 = 165.5 μm
Therefore the averagewidth of one cell is165.5 ÷ 2 =
82.8μm
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