Semester -5th
Paper – Recombinant DNA Technology
Topic - Electrophoresis
BY: KHAN SHAH RUKH
Roll no : 4181457022
Submitted to: Dr. Sweta Yadav
College / Department: MICROBIOLOGY, SSN College ;University of Delhi
First we are very thankful to ‘Delhi University’ who
gave us the path to express our creativity by these
presentations. I also thankful to professor and to the
classmates to see this. This ppt will introduce you
about ELECTROPHORESIS
1. INTRODUCTION
 2. PRINCIPLE
 3. TYPE
 4. EQUIPMENT
 5. AGE
 6. PAGE
 7. APPLICATIONS
 8. LITERATURE/REFERENCE
 9. QUESTIONS

2. PRINCIPLE of ELECTROPORESIS
Cont.
Native
PAGE
SDS
PAGE
IEF
PAGE
2-D
 Electrophoresis
is a seperation technique
 There are a different type of electrophoresis
 Gel electrophoresis is one of the type of
electrophoresis
 There are different componant of electrophoresi
(power pack , electrophoretic chamber , buffers,
gel , etc



o
o
o
o
Molecular Biotechnology by Glick BR, Pasternak JJ & Patten CL, Ed. IV,
ASM Press,2010
www.MolecularCloning.com
Sambrook and Russell Chapter 5,Gel Electrophoresis of DNAand
Pulsed-field Agarose GelElectrophoresis
Biophysics - Vasantha Pattabhi, N. Gautham, 2.3 Electrophoresis page32
Principles of Gene Manipulation and Genomics - Sandy B. Primrose &
Richard Twyman
Gel electrophoresis of nucleic acids - Wikipedia, the free encyclopedia
electroporesis - Google Search