Chapter 3
HISTOLOGY
Introduction
A collection of cells all working together to perform a function is called tissue.
The study of tissues is called histology. The purpose of this lab is to reinforce cell structure and
learn about microscopic anatomy of various tissues using the compound light microscope.
Objectives:
1. Identify epithelial tissues by number of layers, cell shape, and specializations using the
microscope
2. Identify and describe a variety of connective tissues using the microscope
3. Relate tissue structure to tissue function and describe how organs are formed from two
or more tissue types
4. Give examples of organs where each tissue type is found
Prelab Assignment
Prior to beginning of laboratory session, study sections in your textbook titled “The Study of
Tissues”, and “Connective Tissue” (fibrous, adipose, and blood.). Pay attention to tables and
photos.
Become familiar with the appearance and functions of the various tissues.
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HISTOLOGY
Squamous
Cuboidal
Simple
Columnar
Pseudostratified
Epithelial
Tissue
Squamous
Cuboidal
Stratified
Columnar
Transitional
Hyaline
Cartilage
Elastic
Fibrous
Connective
Tissue
Bone
Areolar
Blood
Connective
Tissue Proper
Adipose
Reticular
Elastic
Dense regular
Dense irregular
Smooth
Muscle
Tissue
Skeletal
Cardiac
Nervous
Tissue
Neurons
Neuroglia
Figure 3.1 Flowchart of Tissue Types
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Exercise 1: Epithelial Tissues
Identify the following epithelial tissues under the microscope. Remember that these slides show slices
from an organ, and that organs are made up of several different types of tissues.
For each tissue: 1. Draw the significant features
2. Label the structures listed
3. Name the locations where the tissue is found
A. Simple Epithelium
1. Simple Squamous Epithelium
Label: nucleus of a simple squamous cell
Locations:
2. Simple Cuboidal Epithelium
Label: nucleus of a simple cuboidal cell
Locations:
3. Simple Columnar Epithelium
Label: nucleus of a simple columnar cell. Identify a goblet cell
Locations:
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B. Stratified Epithelium
1. Stratified Squamous Non-keratinized
Label: nucleus of a squamous cell
Outline the epithelium
Locations:
2. Stratified Squamous Keratinized
Label: nucleus of a squamous cell, keratin layer
Locations:
Check Your Understanding
1. Compare and contrast the locations of keratinized versus non-keratinized epithelial tissue.
What is the significant function of keratin?
__________________________________________________
__________________________________________________
2. Site two locations of simple epithelium and two locations of stratified. How does the change in
form modify the function?
__________________________________________________
__________________________________________________
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Exercise 2: Connective Tissue
A. Loose Connective Tissue
1. Areolar
Label: fibroblasts, elastic fibers, collagen fibers.
Locations:
2. Adipose
Label: adipocyte, nucleus
Locations:
B. Dense Connective Tissue
1. Dense Regular
Label: fibroblasts, nucleus, collagen fibers
Locations:
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2. Dense Irregular
Label: collagen fibers
Location:
C. Fluid Connective Tissue
Blood
Label: red blood cells, white, blood cells
Locations:
Check Your Understanding
1. A connective tissue is defined as having cells and extracellular matrix. What is the matrix in
blood? Name two different blood cells and one function for each.
_______________________________________________
_______________________________________________
2. Complete the following table:
Tissue
Location
Simple squamous epithelium
Simple cuboidal epithelium
Simple columnar epithelium
Stratified squamous epithelium
Non-keratinized
Keratinized
Areolar CT
Dense regular CT
Dense irregular CT
Adipose CT
Blood
Function
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